Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf

Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf

Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf

... 2004 Unfolding and stability of Thermotoga maritima HU (Eur. J. Biochem. 271) 1507 Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic ... for increasing the thermal stability of proteins. Materials and methods Overproduction and purification of HU proteins Wild-type...
Ngày tải lên : 23/03/2014, 12:20
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... analyzed by MALDI-TOF MS. The addition of the protease is considered the zero time point. The relative intensities of the peaks from the mass spectra were used to calculate the fraction of Pyr-ONC (M23L), ... (Met1)-ONC (M23L) by the addition of AAP, as described in the Materials and methods. Completion of the hydrolysis was confirmed by the disappearance o...
Ngày tải lên : 19/02/2014, 12:20
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Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

... with MCL. The thermodynamic forces governing the interaction of some of the porphyrins have been delineated from an analysis of the temperature dependence of the association constants. The results ... of MCL and the effect of ligand binding on it, the far-UV CD spectra of MCL in the native state as well as in the presence of CuTCPP and CuTMPyP wer...
Ngày tải lên : 19/02/2014, 16:20
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Báo cáo khoa học: Thermodynamic analysis of Jun–Fos coiled coil peptide antagonists pdf

Báo cáo khoa học: Thermodynamic analysis of Jun–Fos coiled coil peptide antagonists pdf

... dissociating from the DNA. Table 2. Thermodynamic data from ITC, thermal melting analysis and bZIP K D values are shown as derived using ITC data, and in parentheses from data taken using the midpoint of ... cJun and cFos, it is anticipated that we can split the free energy of binding into its thermodynamic components to investigate whether there is a thermodyna...
Ngày tải lên : 06/03/2014, 01:20
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Tài liệu Báo cáo khoa học: Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores pdf

Tài liệu Báo cáo khoa học: Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores pdf

... those formed under a number of stress conditions [22], but with the wall of the pore made of both lipid and pro- tein molecules. At the rim of the membrane hole, the two leaflets of the bilayer can make ... the segments that in the full-length, water-soluble Bax make the fifth and sixth a-helices of the protein structure [25]. However, when these fragments...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khoa học: Arginine ethylester prevents thermal inactivation and aggregation of lysozyme pptx

Tài liệu Báo cáo khoa học: Arginine ethylester prevents thermal inactivation and aggregation of lysozyme pptx

... destabilizes protein structure, we analysed the thermal unfolding profile of lysozyme in the presence of additives. Figure 3A shows the thermal unfolding curve of lysozyme in the presence of ArgEE ... in the presence of ArgEE. The concentrations of ArgEE were shown in the figure. (B) T m in the presence of Arg (d)orArgEE( s). Fig. 4. Thermal unfolding curve...
Ngày tải lên : 19/02/2014, 16:20
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Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc

Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc

... portions of strands B and C that protrude towards the solvent and in the conformation of the long loop connecting strands D and E [17]. Greater differences are observed between the structures of B. ... decarboxylase] involved in the oxidation of uric acid to allantoin. Certainly, the co-localization of these proteins in the peroxisomes of metazoan and plant s...
Ngày tải lên : 07/03/2014, 00:20
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Báo cáo khoa học: Post-ischemic brain damage: pathophysiology and role of inflammatory mediators ppt

Báo cáo khoa học: Post-ischemic brain damage: pathophysiology and role of inflammatory mediators ppt

... represent endogenous mechanisms of recovery and repair. The switch from detrimental to beneficial effects seems to depend on the strength and the duration of the insult and is crucial for determining the time-window ... highlighted the involvement of matrix metalloproteinases in the propagation and regula- tion of neuroinflammatory responses to ischemic brain inj...
Ngày tải lên : 07/03/2014, 03:20
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Báo cáo khoa học: pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I ppt

Báo cáo khoa học: pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I ppt

... picture of the enzyme and should be helpful in a comprehensive under- standing of the role of hKAT-I, especially in KYNA biosynthesis in the human brain. Experimental procedures Enzyme expression and ... shown). Incorporation of a-ketobutyrate or other a-keto acid concomitantly decreased and increased the 335 nm and 422 nm peak, respectively, and the relative dimen...
Ngày tải lên : 07/03/2014, 16:20
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Báo cáo khoa học: Cloning, over-expression, purification and characterization of Plasmodium falciparum enolase doc

Báo cáo khoa học: Cloning, over-expression, purification and characterization of Plasmodium falciparum enolase doc

... fitting the data to t he Michae lis–Menten equation using the SIGMAPLOT software. MALDI-TOF analysis For determination of the exact molecular mass of the expressed recombinant protein, MALDI-TOF mass ... polymerase and 2 lL of the template library in a final volume of 20 lL. The amplified enolase PCR product and the pQE30 plasmid vector were digested with KpnIandPstI...
Ngày tải lên : 16/03/2014, 18:20
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