Báo cáo khoa học: RNA reprogramming of a-mannosidase mRNA sequences in vitro by myxomycete group IC1 and IE ribozymes pptx

... al.2800 FEBS Journal 273 (2006) 2789–2800 ª 2006 The Authors Journal compilation ª 2006 FEBS RNA reprogramming of a-mannosidase mRNA sequences in vitro by myxomycete group IC1 and IE ribozymes Tonje ... April2006)doi:10.1111/j.1742-4658.2006.05295.xTrans-splicing group I ribozymes have been introduced in order to mediate RNA reprogramming (including RNA repair) of therapeutically relevant RNA transcripts. Efﬁcient RNA reprogramming depends ... presentation of the group I ribozyme-mediated trans-splicing reaction resulting in RNA reprogramming of a-mannosidase mRNA. The trans-splicing ribozyme construct base pairs to mRNA sequences upstream of...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... DNA-binding motif in nucleolin [21–27]. The RGG domain of FMRP, which is an RNA- binding protein involved in nerve cell differentiation,interacts with the G-quartet forming RNA [22–25]. In addition, ... guanidini-um group of the Arg in the RGG domain in theC-terminal of EWS.Results and DiscussionSeveral DNA-binding proteins that bind to G-quadru-plex DNA have been investigated in vitro ... subgroup within the RNP family of RNA- bindingproteins containing RRM and RGG domains, binds totelomeres. Further studies are required to identify therole of EWS and the possible function of...

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... examined cal-pain 7–IST1 interactions by in vitro and in vivo bindingexperiments, using puriﬁed recombinant proteins and cultured mammalian cells expressing epitope-taggedproteins. We also investigated ... autolysis of mGFP–cal-pain 7–Strep by GST–IST1 and GST–MIM in vitro. Fig. S2. Intermolecular proteolysis of mGFP–cal-pain 7C290S–Strep and mGFP–calpain 7 DMITC290S–Strep by Strep–calpain 7 in vitro. Table ... contains both mGFP and MITdomains of calpain 7. On the other hand, the anti-GFP-reacting 30 kDa band was not detected in theimmunoprecipitates of antibody against calpain 7,indicating a lack of...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... protein concentra-tion was determined using the Coomassie Plus Protein AssayReagent (Pierce) with bovine serum albumin as the standard.Expression and purification of recombinant protein wasconfirmed ... that of the enzyme-containing solution and was expressed as nmols of 2,6-dichloroindophenol reduced per mg of protein permin. The extinction coefficient of 2,6-dichloroindophenol(21 000 MÆcm)1) ... leucine degradation and thesku mutant accumulates branched-chain amino acids in haemolymph, thismutant may be useful in the investigation of unique branched-chain aminoacid catabolism in insects.AbbreviationsACAD,...

Tài liệu Báo cáo khoa học: Molecular determinants of ligand speciﬁcity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc

... screening and identifying ligand binding toprotein receptors. Angewandte Chemie-International Edi-tion 42, 864–890.16 Mayer M & Meyer B (1999) Characterization of ligandbinding by saturation ... was minimized in twostages. In the first stage, we kept the protein fixed and onlyminimized the position of the water molecules and ions. In A. Viegas et al. Determinants of ligand specificity in ... could indicate that the corre-sponding hydroxyl groups are involved in proteinbinding.The results for the linebroadening measurements of protons H1a in the alpha and beta configurations,aHa1 and...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... in ammatory cytokines, including IL-1b, TNF-a and IFN-c. (B) Chemokines expressed in the joint recruit leukocytes intothe joints. In addition to functioning in cell trafﬁcking, severalchemokines have ... IFN-c, interferon-c; IL, interleukin; IP-10, interferon-c-inducible protein-10; MAPK,mitogen-activated protein kinase; MCP, monocyte chemoattractant protein; Mig, monokine induced by interferon-c; ... levels of IL-1b, IL-6 and IL-8 ⁄ CXCL8 in culture supernatants of synoviumfrom RA patients, and the expression of MCP-1 ⁄CCL2 mRNA by cultured synovial cells is stimulated by IL-1b and TNF-a...

Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx

... that the N-terminal domain is involved in protein–protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspeciesinteractions are incompatible.From ... [15–18]. In thebovine complex a large subdomain is made up of  20integral membrane proteins contributing > 60 transmem-brane segments. Some of these must be intimately involved in proton pumping. ... proceduresCell lines and cell cultureThe isolation and preliminary biochemical and geneticcharacterization of a series of respiration-deﬁcient Chinesehamster mutant cell lines has been described...

Tài liệu Báo cáo khoa học: "The Impact of Query Reﬁnement in the Web People Search Task" docx

... clustering task, with over 15 research groupssubmitting results in each campaign. Since the re-lease of the ﬁrst datasets, this task is becoming anincreasingly popular research topic among Infor-mation ... types of features from eachdocument. First, we applied a simple preprocess-ing to the HTML documents in the corpus, con-verting them to plain text and tokenizing. Then,we extracted tokens and ... the goal is to mine theweb for information about a person. In a typicalhiring process, for instance, candidates are eval-uated not only according to their cv, but also ac-cording to their web...

Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc

... (Table 1). The binding affinities of the inhibitorto both binding sites, yielding the IE or EI complex, wereonly slightly different. On the other hand, the bindingaffinity of the second inhibitor ... Ala743 of the enzyme, betweenthe side chain carbonyl group of Asn7 of Tat(1–9) and theside chain of Lys554 of DP IV and finally, between thecarbonyl group of Ile8 of the peptide interacting with ... N-terminus (Met1) of Tat(1–9) and Asp709 as well as Asp739 of DP IV, between the C-terminalGlu9 of Tat(1–9) and the side chains of Arg560 and Lys554 of DP IV. Furthermore, the side chain of Asp5 of...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... thisprotease at pH9 [10]. Further interest in the aromaticstacking is in the role of the electrostatic properties in enzymatic catalysis of API, and in distinguishing thefunctionally catalytic ... conﬁrmed by energy minimization (Fig. 3). The chargeinteraction between Asp113 and His210 is weakened withincreasing solvent-accessibility of the His210 side-chain. In the protein interior, the dielectrostatic ... in bovine trypsin and API. The catalytic triadresidues of trypsin and API are Ser195–His57–Asp102 and Ser194–His57–Asp113, respect-ively. The substrate-binding subsite residues of trypsin and API...

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