Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt

... the corresponding orientation of the side chain of Glu221. The second conformation of the side chain of Glu221, which also adopts twoconformations but not correlated with the anomeric form of ... Authors Journal compilation ª 2006 FEBS Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin Lieven Buts1, Abel Garcia-Pino1, Anne Imberty2, ... GlcNAc in the )1 subsite of PAL is energetically favorable and contri-butes signiﬁcantly to the higher afﬁnity of PAL for the pentasaccharide M592 (Table 3). In the case of con Aon the other hand,...

Báo cáo khoa học: Structural basis for the erythro-stereospeciﬁcity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

... results from conformationalcontrol of the enzyme on the side chain rotamer of the bound substrate. For example, in AsnO, a trans con-former is selected for the v1torsion angle of boundl-asparagine, ... inactive site formation by bordering the substrate boundtherein.Active site of VioCCrystals of the substrate complex were obtained by crystallization of puriﬁed VioC in the presence of potassium ... conformers of the substrate are suitablyoriented to point with the proS-hydrogen atom of the Cb group towards the catalytic iron. With an iron–hydroxyl distance of 3.1 A˚, the structure of the VioC•hArg•Fe(II)•succinate...

Báo cáo khoa học: Structural basis for the interaction between dynein light chain 1 and the glutamate channel homolog GRINL1A docx

... peptide:proteinratios of 0.5 and 1, corresponding to the free andbound forms, respectively. Their intensities varyaccording to the population fractions of the free andbound forms of DYNLL1 at the various ... area.Figure 4A shows the 20 lowest-energy conformersfrom the selected cluster modelled using the Haddockdocking calculations. The rmsd values for the 20lowest-energy conformers of the best cluster ... randomlydeleted for each docking trial. Final cluster analysis wasperformed to evaluate the structure quality of the dockedcomplexes. The patterns of intermolecular interactions for the full-length...

Báo cáo khoa học: Structural basis for the changed substrate speciﬁcity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx

... comparing the phosphorylation of the natural substrates of the double mutant with the singlemutant, it seems that the mutation N45D in the double mutant counteracts the negative effect(s) of the N64D ... ion in the P-loop andGlu172 to the 3¢-OH of the substrate. In the dTTPcomplex, the phosphates are bound by the LID argi-nines and the 3¢-OH is bound to Glu172. In thesecases, Glu171 in the LID ... is there-fore a particularly attractive candidate for the medicalgene therapy applications mentioned above, as well as for industrial synthesis of d(d)NTPs and their analogs[6,15]. To further...

Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt

... half-buried, and the form of the cleft is markedlydifferent from those of TvuCMBP and EcoMBP.These observations suggest that the structure of the N-domain part and the capacity of the sugar-bindingsite ... complex, the specificity of the bacterial sugar-binding proteinsshould be defined in terms not of the affinities for sugars but of whether the protein adopts the openform or the closed form. TvuCMBP–c-CD ... and the conforma-tions of the residues at regions N-I–III are different, asdescribed above. The capacities of the sugar-binding sites of TvuCMBP, EcoMBP and TliTMBP, where all of the conformations...

Báo cáo khoa học: Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase potx

... chaintakes place at the second glycosidic linkage from the MeGlcA side group towards the reducing end of the xylan chain. The elucidation of the three-dimensionalstructure of the E. chrysanthemi GH30 ... char-acter of the substrate, this Xyl becomes the product of hydrolysis or the nonreducing end of the leavinggroup. A stereo view of the mode of binding of MeGlcA2Xyl3is shown in Fig. 5A. The interactions ... however, the rate of their hydro-lysis with XynA was negligible in comparison with the rate of hydrolysis of aldouronic acids.After the 3D structure of the enzyme becameknown [16] and the mode of...

Báo cáo khoa học: Structural basis for recognition of Co2+ by RNA aptamers pot

... hybridize to the 5¢-end of the loops and propagate to nucleotides involved in the formation of the helix. The strong preference for the 5 ¢-side of the loops in comparison with the 3 ¢ -sidemay ... Participation of the N7 atom of purines in the organization of the ter-tiary structure of other RNAs has been extensivelyinvestigated by the NAIM method. Heide et al. [26]have demonstrated the signiﬁcance ... suggest that these guanine bases are crucial for the creation of Co2+binding sites and that they appear to be involved in the coordination of the ion to the exposed N7 atom of the tandem guanines....

Báo cáo Y học: Structural basis for the inhibitory efﬁcacy of efavirenz (DMP-266), MSC194 and PNU142721 towards the HIV-1 RT K103N mutant doc

... reduced by the s tacking of O d1 in between the plane of the thioureamoiety and the Ca of G190.Hence, the undefined orientation of the N103 amide Od1and Nd2 atoms may r eflect the repulsive forces ... electron density for the a mide part of the residue is not very well defined for any of the inhibitorcomplexes, the inhibitors can still be clearly ranked withrespect to the extent of the van der ... induced by the K103N mutation The e lectron density fo r Cc of the K 103N mut ant st ructuresis well defined but the quality of t he map does n ot allowassessment of the orientation of the amide...

Báo cáo khoa học: Structural basis for stereo-speciﬁc catalysis in NAD+-dependent (R )-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans pptx

... binding of coenzyme and substrate to the enzyme in its open con-formation followed by the closure of the interdomaincleft. In FDH, the structural ordering of the C-ter-minal helix and the rotation ... exit by a reopening of the interdomain cleft.In addition to explaining the functionality of the catalytic triad, the structure–function relationshipsobtained by the comparison of HGDH with other ... aresolution of 1.98 A˚. The structural analysis of HGDHtogether with the comparison with other structurallycharacterized members of the D-2-hydroxyacid dehy-drogenase protein family further contributes...

Báo cáo khoa học: Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2) potx

... demonstrated the transport of conjugated and unconjugated lipophilic anions by MRP2. The absence of MRP2 from the canalicularmembrane of human hepatocytes is the molecular basis of the Dubin–Johnson ... N-terminus of MRP2. The N -terminus of MRP2 is located on the extracellular s ide[16], therefore a cDNA was constructed which led totranslation of a GFP inserted into t he lumen of the ER by themurineIgj-chain ... of a MRP1/2 chimeric proteinin polarized MDCKII cells The amino-acid identity of only 48% between the laterallylocalized isoform MRP1 a nd the a pically localized isoformMRP2 [1] hampers the...

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