Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf

... key role in minimizing cell injury and maintaining tissue integrity in response to damaging levels of an environmentalagent. As such, the integrity of this system plays a role in modifying progression ... clinical model that is giving novel molecularmechanistic insight is adenocarcinoma of the oesopha-gus [2]. This cancer is one of the fastest rising cancers in the west, is taking the place of ... made using the protein kinase C (PKC) inhibitor bisindolylmalei-mide I (Bis-I), which inhibited DCA-dependent apop-tosis (Fig. 3I). The control inactive version of the inhibitor bisindolylmaleimide...

Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx

... for 2 h) cells, indicating that interaction with Smac is not required with respect to the role of HSP70 in the inhi-bition of the release of Smac and apoptosis. The role of the ATP-binding domain ... interaction of HSP70 with apoptosis signal-regulating kinase 1 (ASK1) and the inhibition of ASK1-induced apoptosis in vitro [28]. Furthermore, the ATP-binding domain of HSP70 is critical for sequester-ing ... apoptosis-inducing factor (which activate caspasecascades) through the mitochondrial transition pore[30]. Bcl-2 is the prototype of the bcl-2 family of proteins and is distributed in the mitochondria,endoplasmic...

Báo cáo khoa học: Caspase-8- and JNK-dependent AP-1 activation is required for Fas ligand-induced IL-8 production ppt

... activities of FasL is to induce in ammation in vivo [15–18], and this in ammatoryactivity of FasL seems to play deleterious roles in inﬂammatory diseases [19–21]. Consistent withits in ammatory activity, ... death effector domain (DED) of FADD], forming death-inducingsignaling complex [1–4]. Depending on the cell type, the upstream caspases activated in this complex in turninitiate the activation ... FP1mutant inhibited the FasL-induced AP-1 activation.These results indicate that the C-terminal 15 aminoacids of Fas are dispensable, but the DD of Fas is indispensable for its ability to activate...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... 132 amino acid domain C-terminal to the HD in Meis2d. We therefore suggest that the autoin-hibitory function of the Hth domain in Meis2d is likely to be a common feature of Meis family proteins.Switching ... similar isoform. It has been suggestedthat Pbx proteins are the major DNA-binding partners for Meis proteins, consistent with the presence of anintact Hth domain in the majority of Meis isoforms[34]. ... deletion of the Hth domain, suggestingthat the inhibitory effect of this domain is notspeciﬁc to the Meis2d AD. Comparison of the relativeexpression levels of these GBD fusion proteins (seeFig....

Báo cáo khoa học: Structure–function analysis of the ﬁlamentous actin binding domain of the neuronal scaffolding protein spinophilin pot

... studies of the actin binding domain of spinophilin. We demonstrate that the spinophilinactin binding domain is intrinsically unstructured, and that, with increasingC-terminal length, the domain ... spinophilin sequence with the positions of the construct limits used in this study and domain borders indicatedby numbers. The core actin binding domain, PP1 binding domain, PDZ domain and C-terminal ... regions of dis-order in the ABDs of spinophilin and neurabin. Asshown in Fig. 1, these programs also predicted a highdegree of disorder in the ABDs of spinophilin andneurabin. On the basis of these...

Báo cáo khoa học: Monomeric solution structure of the helicase-binding domain of Escherichia coli DnaG primase pdf

... besides occurring in DnaG C-terminal domains, has beendescribed only for the N-terminal domain of DnaB. The C-terminal helixhairpin present in the DnaG C-terminal domain, however, is either less ... helicase forms a hexameric ring structurewith up to three molecules of the DnaG primaseattached [2–4]. DnaG is composed of three maindomains comprising an N-terminal zinc-binding domain for interaction ... sub-domains. Whereas the interaction seems to be entirelyconﬁned to the C-terminal domain of DnaG [4,10], the situation is much less clear for DnaB. For example,mutations in the N-terminal domain...

Báo cáo khoa học: Structure and topology of the transmembrane domain 4 of the divalent metal transporter in membrane-mimetic environments docx

... program of CYANA(1.0) [31]. On the basis of thesedistance constraints obtained using the macroCALIBA,asystematic analysis of the local conformation around the Caatom of each residue, including ... detected in any of the 16 conformers, while the H-bonds between Asp14 and Phe18 were missing in the majority of the 16 conformers, indicating that the structuresmay be distorted in this region. ... to explore the molecular biology aspects of DMT1 since the discovery of this gene, there has so far been no structuralcharacterization of either this integral protein or a segment of it. Analysis of the...

Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx

... This is indicated by the averageactivity of mutants in the central domain of 26% for WAF1 and 34% for MDM2, 71% for NOXA and 61% for p53R2, and around 45% for the other four promot-ers. For ... binding and 244 and 246are in very close proximity to DNA binding. This indi-cates that p53R2 either does not use these residues for binding or that they are not necessary for binding as the DNA ... residue 283 is involved in DNA binding. However, residues 272 and 276 are close to DNA binding. The other four residues are furtheraway, but at the same side of the protein, indicating apossible...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity of the pseudouridine synthase RluD in Escherichia coli doc

... appears to recognize its substrate by indirectreadout of a protein induced RNA structure [21]. It is therefore interesting to note that hairpin 35 of the 23SrRNA (another substrate for RluA) ... the pseudouridine formation may liewithin the ﬂipped out conformation of H69 becausebase ﬂipping is obligatory for Y synthesis [4]. The cocrystal structure of E. coli TruB bound to the T-stem ... found in the 23S rRNA variant containing the double mutationU1911C ⁄ U1915C (Fig. 3). Thus, isomerization of uri-dines 1911 and 1917 in H69 occurs autonomously of each other and is independent of...

Tài liệu Báo cáo khoa học: Receptor binding characteristics of the endocrine disruptor bisphenol A for the human nuclear estrogen-related receptor c pptx

... environments of BPA in the ligand-binding pocket of the ERRc. The proximity of each amino acid residue (within adistance of 5 A˚) to BPA is shown in the boxes depicting the a -heli-ces. The portrait ... group of bisphenol A and estrogen-related receptor c residues Glu275and Arg316 in the ligand-binding domain. By inducing mutations in otheramino acids, we evaluated the change in receptor binding ... inverse agonist activity of 4-hydroxytamoxifen. However, the intrinsic binding mode of bisphenol A remains to be clariﬁed. In the present study, we report the binding potentials between the phenol-hydro-xyl...

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