Báo cáo khoa học: The potyviral virus genome-linked protein VPg forms a ternary complex with the eukaryotic initiation factors eIF4E and eIF4G and reduces eIF4E afﬁnity for a mRNA cap analogue ppt

... The Authors Journal compilation ª 2006 FEBS The potyviral virus genome-linked protein VPg forms a ternary complex with the eukaryotic initiation factors eIF4E and eIF4G and reduces eIF4E afﬁnity ... increasing amounts of pep4G.⇔ VPg eIF4E ⇔ eIF4E- cap eIF4E -VPg cap -eIF4E -VPg VPg⇔⇔ eIF4G -eIF4E- cap Scheme 2. Hypothetical pathways of eIF4E and eIF4G recruitment.Large arrows highlight the connections ... binding of the 40S ribosomal subunit to the mRNA [8]. The general structural similarity between the poty- virus RNA and eukaryotic mRNAs suggests that VPg may act functionally as a cap- like structure....

Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt

... identify the extracellular ligands of the RPTPs in the neuromuscular system. For example, it is knownthat PTPd and an isoform of LAR can interacthomophilically [39] and that LAR can also bindheterophilically ... membranes [60] and in developing skeletalmuscle [37]. The basement membrane-associated lig-ands have been identified as HSPGs. Here, using affin-ity chromatography, tandem MS and RAP affinity assays, ... washed, mounted and analyzed as described above.To semipermeabilize cells for intracellular staining, cellswere incubated in 4% paraformaldehyde for 30 min at 4 °Cin the first instance and then...

Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot

... cotransduction of SUMF1.AbbreviationsAAT, a 1-antitrypsin promoter; AAV, adeno-associated virus; CMV, cytomegalovirus; EF1, elongation factor 1a; GALNS, N-acetylgalatosamine-6-sulfate sulfatase; ... were carriedout in duplicate. Total DNA and RNA were isolated with the AllPrep DNA ⁄ RNA miniprep kit (Qiagen, Valencia,CA, USA), according to the manufacturer’s instructions.Viral DNA was ampliﬁed ... SUMF1 cDNA.Production and puriﬁcation of AAV vectorsAAV vectors were produced by calcium phosphate-medi-ated cotransfection of pAAV–CMV–GALNS, pAAV–AAT–GALNS or pAAV–CMV–SUMF1 with pXX2 and pXX6-80...

Báo cáo khoa học: "Impact of computerized physician order entry on medication prescription errors in the intensive care unit: a controlled cross-sectional trial"

... defined as calculated creatinineclearance less than 50 ml/minute. The parameters needed tocalculate the creatinine clearance were always available inboth the PB-U and the C-U. In addition to the ... the study. KC wasresponsible for conceiving the study, data acquisition, analysisof the data, statistical analysis and drafting of the manuscript.BC was responsible for data acquisition, analysis ... MPEs.Nonparametric data were analyzed with the Kruskal-Wallis and Mann-Whitney U tests. These data are presented as medianvalues (with 25th and 75th percentiles). Nominal data werecompared by using chi-square...

Báo cáo khoa học: "Veterinary decision making in relation to metritis - a qualitative approach to understand the background for variation and bias in veterinary medical records"

... validdata at the herd level. They perceive the collection of the data in and of itself as the basis for taking relevant actionat the farm. They may skip the process of systematic anal-ysis of data ... treat-ment and potential links to data quality. This understand-ing provides insight into potential errors (bias and random error) related to data based on clinical examina-Acta Veterinaria Scandinavica ... shouldclearly be improved before analysing the data on nationallevel.Causal analysis of cow-level and herd-level risk factors for metritis at the national level based on Danish central database...

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... 5¢-CCTTCA ATG ACA ACG ACA CCG-3¢ (forward) and 5¢-CCATGC GAC TTG TCA GGC T-3 ¢ (reverse); glyceraldehyde-3-phosphate dehydrogenase, 5¢-GAC ATC AAG AAGGTG GTG AAG C-3¢ (forward) and 5¢-GTC CAC CACCCT ... Technology).Nuclear extract preparation and EMSA For nuclear extract preparation, cells were harvested and washed twice with cold PBS. The nuclear extract was pre-pared as described previously [11]. EMSA was ... antibody.RNA extraction and real-time PCRTotal RNA was isolated using TrizolÒreagent (Invitrogen)in accordance with the manufacturer’s protocol. Afterextraction, 5 lg total RNA was then used as a...

Tài liệu Báo cáo khoa học: It is all about resolution Meeting report based upon presentations at the 10th International Global BioMillennium 2006 symposium on molecular cell biology (Tbilisi, Georgia) docx

... properties and intracellular localization.DAP-kinases (DAPk), Ca2+⁄ calmodulin-regulated and Ser ⁄Thr kinases, activate signaling pathways thatlead to cell death through membrane blebbing and autophagic ... mediated by nuclear porecomplexes, macromolecular assemblies that fuse the inner and outer nuclear membrane and form aqueouschannels for translocation. Ohad Medalia (Beer-Sheva,Israel) reported ... by A. Francis Stewart(Dresden). Silencing methylations are epigeneticallymaintained via methyltransferases that associate with proteins that recognize the methylated epitope and propagate the...

Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt

... mimick the heme cofactor and the axial histidine ligand of the iron in peroxidases,whereas a COOH side chain of the antibody acts as a general acid±base catalyst in the same manner as the distalhistidine ... 1a Ln2where DA  A ) A 0, DAI A ) A I and A 0, A I,andAare the absorbances of the initial, ®nal and mixed species,respectively. The linearity of the graph representing 1/DA as a ... of the antibody and particularlyto appreciate the size of the cavity left around the iron atom and which kind of ligands it can accommodate; second, tomeasure the in¯uence of an axial ligand...

Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx

... of the University of Balamand and complied with the principles of laboratory animal care.Chemicals and reagentsMuramyl peptides (MDP and MB) used in this work werekindly provided by ISTAC-SA ... produc-tion after stimulation plays a pivotal role in numerous and diverse biological functions, in particular as a principal mediator of the microbicidal and tumoricidalactions of macrophages [31]. ... upregulation cor-related with free radical generation, cells stimulated with MDP were pretreated with an antioxidant (vita-min E). Figure 5A shows that both OÀ2 and total NOsigniﬁcantly decreased...

Báo cáo khoa học: Cysteine residues exposed on protein surfaces are the dominant intramitochondrial thiol and may protect against oxidative damage docx

... this, the exposed thiol willpreferentially sustain the oxidative damage, ratherthan another amino acid, as a result of its greaterreactivity with most damaging species, thereby actingas a local ... and may play a critical role inintracellular antioxidant defences against oxidative damage.AbbreviationsACA, e-amino-n-caproic acid; AMS, 4-acetamido-4¢-maleimidylstilbene-2,2¢-disulfonic acid; ... centrifugations, and from a mitochondrial fraction, iso-lated from liver (C) and heart (D) tissue homogenates. The percentages above the data bars indicate the total GSH content of the fraction as a percentage...

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