Báo cáo khoa học: Synthesis and structural characterization

Báo cáo khoa học: Synthesis and structural characterization of a mimetic membrane-anchored prion protein doc

... compilation ª 2006 FEBS 1299 M. R. Hicks et al. Lipid-anchored PrP Synthesis and structural characterization of a mimetic membrane-anchored prion protein Matthew R. Hicks 1 , Andrew C. Gill 2 , Imanpreet ... surface and an endocytic compartment in cultured neuroblastoma cells. J Biol Chem 268, 15922–15928. 19 Naslavsky N, Stein R, Yanai A, Friedlander G & Tara- boul...

Ngày tải lên : 23/03/2014, 10:21

15
425
0

Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deﬁcient families from Southern Italy docx

... S, Dianzani I, Lattanzi P, Spada M, Romano V, Calı ` F, Andria G, Ponzone A, Marra E & Piazza A (2001) Genetic heterogeneity in ﬁve Italian regions: analysis of PAH mutations and minihaplo- types. ... Italy. The study was partly supported by Agenzia Italiana del Farmaco (AIFA grant FARM5MATC7), Rome, Italy. We thank Jean Ann Gilder for revising and editing the text and Anna Nas...

Ngày tải lên : 16/03/2014, 01:20

12
491
0

Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx

... 2495 Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus Giovanna Cacciapuoti 1 , Sabrina Gorassini 1 , Maria Fiorella Mazzeo 2 , ... (time-zero control) and at different time inter- vals, aliquots were removed from each sample and analyzed for activity in the standard assay. Activity values are expres...

Ngày tải lên : 23/03/2014, 09:21

14
384
0

Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

... homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS chloroplasts were used as starting material for the separation ... chloroplasts were separated in CN ⁄ PAGE (1D, the hori- zontal bands) and then in nondenaturing conditions by BN ⁄ PAGE (2D). The molecular mass standards and direction o...

Ngày tải lên : 23/03/2014, 13:20

12
431
0

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... putative allantoate amidohydrolase, which is part of the urate catabolic pathway in many organisms [8]. In fact, by genome data mining, another hydantoinase (HYD) was also found in the Jannaschia ... (1988) Stereo- and substrate-speciﬁcity of a d-hydantoinase and a d-N-carbamyl amino acid amidohydrolase of Arthrobacter crystallopoietes AM 2. Enzyme Microb Technol 10, 618–625....

Ngày tải lên : 18/02/2014, 08:20

14
621
0

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... developmental cycle: early, middle and late substrate colonization stages (4, 8 and 12 days); pinhead stage (day 14), button stage (day 18), egg stage (day 21), elongation stage (day 22) and mature stage ... cycles of 94 °Cfor20s,52°Cfor20s and 70 °C for 2 min; then a ﬁnal extension at 72 °C for 10 min. The primers for lac1 P LAC1F (5¢-AGCTTT CATTCCCAGTGATTG-3¢)andP LAC1R (5¢-AACG...

Ngày tải lên : 07/03/2014, 15:20

11
703
0

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... 5¢-CTAGACTCGAGCCTAAT TTATATTTGCTCCTTGTGC-3¢. b-Actin primers were designed as follows: forward 5¢-CTACAATGAGCTGCG TGT-3¢ and reverse 5¢-AAGGAAGGCTGGAAGAGT-3 ¢. Cell survival and apoptosis analysis For viability ... regulation of caspases. Taken together, these results indicate that VBARP is a novel splice variant of ANKHD1 and may play a role in cellular apoptosis (antiapoptotic...

Ngày tải lên : 07/03/2014, 21:20

12
561
0

Báo cáo khóa học: Overexpression and enzymatic characterization of Neisseria gonorrhoeae penicillin-binding protein 4 ppt

... of 90 min. D -Ala product was detected with QuantaBlu TM assays. j, Boc-Cbz-KAA; r, Boc-Ac-KAA; s, Ac-C-KAA; n, Ac 2 -KAA; h, Boc-H-KAA; d, Ac 2 -KA- D -Lac. (A) High activity range showing activity ... TEM b-lactamase, EC PBP 5, NG PBP 3, and NG PBP 4. NA, data not available; AA, amino acid. pK a Enzyme TEM b-Lactamase EC PBP 5 a NG PBP 3 b NG PBP 4 c pK a value AA residue pK a v...

Ngày tải lên : 23/03/2014, 12:20

10
328
0

Báo cáo khoa học: Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley pot

... violaxan- thin, zeaxanthin and b-carotene, 3 for lutein and Chl b, and 7 for total carotenoid content (Tot Car). LL, low light; HL, high light; VAZ, violaxan- thin, antheraxanthin and zeaxanthin. Chl ... monomeric antenna proteins (CP26, CP29 and LHCII monomers) (band 2), and tri- meric LHCII (band 3), monomeric (band 4) and dimeric (band 5) PSII cores. Bands 6 and 7 contained...

Ngày tải lên : 30/03/2014, 10:20

15
476
0

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... development and use of N-acylsulfonamides and sulfonimides as antagonists of nucleic acid-binding proteins. Database Structural data for the two RNase A complexes are available in the Protein Data Bank ... Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A Nethaji Thiyagarajan 1 , Bryan D. Smith 2, *, Ronald T. Raines 2,3...

Ngày tải lên : 14/02/2014, 22:20

9
626
0