Báo cáo khoa học: Protective effects of endomorphins, endogenous opioid peptides in the brain, on human low density lipoprotein oxidation pdf

... Authors Journal compilation ª 2006 FEBS Protective effects of endomorphins, endogenous opioid peptides in the brain, on human low density lipoprotein oxidation Xin Lin1, Li-Ying Xue1, Rui Wang1,2, ... peroxidation of LDL and its inhibition by endomorphins and GSH. The reaction con-ditions and the initial concentration of the substrates are the sameas described in the legends of Fig. 1 for reactions ... diseases. Therefore, protecting LDLfrom oxidation may be essential in the brain. The antioxidative effects of endomorphin 1 (EM1) and endomorphin 2 (EM2), endogenous opioid pep-tides in the brain, on...

Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc

... http://www.cgl.ucsf.edu/chimera/) [69] of the H9-H9¢ loop (dashed box in A),which contains a proline at position 303 in c-T1 (A¢) in contrast to the serine of c-T2 (A¢¢). The proline substitution of c-T1 eliminates the hydrogen ... sub-stitution at position 72: glycine in place of the c-T1 ⁄ consensus arginine (Figs 2–4). The presence of glycine at this position in c-T2 has only beenobserved in E. focardii and in the psychrotolerantEuplotes ... ﬁrstintron included nucleotides 50–96 and the second intronincluded nucleotides 210–253 in each gene. Excludingintrons and stop codons, the c-T1 and c-T2 codingregions were each 1383 bp in...

Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus inﬂuenzae pot

... shown); the exact location is a matter for further investigation. The low abundance of the sialylated glycoforms preven-ted the determination, by NMR, of the location of Neu5Ac.Precursor ion monitoring ... observed corresponding toglycoforms containing only one PEtn group or containingone PEtn group and a phosphate group. In order to obtain sequence and branching information, the OS fractions were dephosphorylated ... observed corresponding to the proposed compositions butadditionally containing glycine or a phosphate group. Minor peaks could also be observed corresponding to glycoforms containing only one PEtngroup...

Báo cáo khoa học: Identiﬁcation of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt

... blotting in combination with densi-tometric scanning of the bands on the blotting membrane. The enzymatic activities were correlated with the concen-tration of recombinant proteins in the media. ... catalytic site of GalNAc-T1, might beinvolved in the catalytic function of the enzyme.To investigate whether the cysteine residues modiﬁed byPCMPS are involved in the binding of UDP-GalNAc,we ... from inactivation, to the same extent. This suggests that GalNAc-T1 contains freecysteine residues interacting with the UDP moiety of the sugar donor. For the functional analysis of the cysteineresidues,...

Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf

... overexpression of enolase 1and malic enzyme 1 supports the induction of fattyacid metabolism. The reduction in d-amino acid oxi-dase 1 expression in the kidney proteome is interesting, in view of the ... detected in knock-out mice. In 14 of the 18 spots, MALDI peptide massfingerprints allowed the identification of the correspond-ing proteins in the database (Table 2). Database searchand functional ... to our study and the inferences that can be based on our findings. Furtherstudies of the sources of endogenous oxalate synthesis in humans are needed. The phenotypic features of Agxt) ⁄ )mice...

Báo cáo khoa học: Bioenergetic requirements of a Tat-dependent substrate in the halophilic archaeon Haloarcula hispanica pdf

... wasthen measured using the starch–iodine method.Minimal inhibitory concentrations of ionophoresTubes containing 5 mL of rich medium containing variousconcentrations of ionophores were inoculated ... processes in prokaryotes. To investigate the effect of ionophores on the secretion of AmyH, we ﬁrst measured the minimalinhibitory concentration (MIC) of several ionophores,and then monitored the ... translocationIt is conceivable that the effect of monensin on AmyHsecretion is not directly due to dissipation of the SMF.We have, for instance, observed that addition of highconcentrations of monensin...

Báo cáo khoa học: Light regulation of CaS, a novel phosphoprotein in the thylakoid membrane of Arabidopsis thaliana doc

... dominatedunder high-light conditions in the wild-type (Figs 1Aand 4B) and the stn7 mutant (Fig. 4B). The involvement of STN8 in the phosphorylation of CaS was further investigated by isolation ... suggest eitherthat CaS is a direct target of the STN8 protein kinase orSTN8 is a crucial component of the protein phosphory-lation cascade involved in CaS phosphorylation.Characterization of the ... protein, we then tested whether the absence of CaS exerts any effects on the composition of the thylakoid protein complexes. To this end, an immu-noblot analysis was performed on the contents of...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... secondsubunit. The backbone NH group of R98 forms ahydrogen bond with the backbone CO of F102. Fur-thermore, the orientation of the side chain of the tworesidues brings the guanidinium plane and the ... in the vicinity of the active site that serve to position the functional K12 residue. The network of key interactions spans the interacting subunits. The Y74W* mutation can perturb orientations ... interaction (Fig. 9B) [49]. The above discussion rationalizes the observed effects of the Y74W mutation in PfTIM on the stability of the dimeric structure and catalytic activity. Examination of the...

Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt

... 4). The bactericidal effects of H4-(86–100) and ciprofloxacinwere completed within 30 min, as incubation of the cells for a longer period (2 h) in the presence of increasing concentrations of the ... layerand their invasion into the lower parts of the gastro-intestinal tract [7]. Their finding of buforin-I in porcine, bovine and human stomach lavage fluids sup-ported the concept that such a defense ... compilation ª 2008 FEBS 5289short-lived and do not induce cell killing per se, butthey allow the entry of the cationic peptides into the cells and their interaction with cytoplasmic polyanions,nuclear...

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... forma-tion, with the level of foci formation obtained for the empty vectorcontrol point set to 100%. In the SRblow point, one-ﬁfth the usualamount of SRb expression construct was introduced. The ... shown on the right (kDa). The sample in the ﬁrstlane represents an in vitro transcription ⁄ translation reaction in which there was no input plasmid. Of note, a doublet is oftendetected in the ... disparities in theirexpression levels at the onset of foci formation. Assuch, we generated several expression constructs forMxi1-SRa containing different lengths of 5¢-UTR, andtested these in in vitro...

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