Báo cáo khoa học: The crystal structure of a hyperthermostable subfamily II isocitrate dehydrogenase from Thermotoga maritima pdf

... 5’-CACTCTCGAAGAGTTCATAAACGAAGTGAAGAAGAATCTC-3’5’-GAGATTCTTCTTCACTTCGTTTATGAACTCTTCGAGAGTG-3’M. Karlstro¨m et al. Structure and thermal stability of T. maritima FEBS Journal 273 (2006) 2851–2868 ª 2006 Karolinska ... [17].Table 3. Primer sequences.TmIDH mutant Primer sequenceR186M 5’-CCTGGAGAAATCGATCATGAGCTTCGCTCAGTCGTG-3’5’-CACGACTGAGCGAAGCTCATGATCGATTTCTCCAGG-3’F205M 5’-AAAAGGTCGACATCTGGATGGCGACGAAAGACACGATC-3’5’-GATCGTGTCTTTCGTCGCCATCCAGATGTCGACCTTTT-3’D36N ... 5’-AAAAGGTCGACATCTGGATGGCGACGAAAGACACGATC-3’5’-GATCGTGTCTTTCGTCGCCATCCAGATGTCGACCTTTT-3’D36N + D341N D36N-15’-CATCCTTCCCTATCTCAACATCCAGCTGGTTTACT-3’D341N-15’-AAGGGGAGAACTCAACGGAACACCGGAGG-3’D389N 5’-CACTCTCGAAGAGTTCATAAACGAAGTGAAGAAGAATCTC-3’5’-GAGATTCTTCTTCACTTCGTTTATGAACTCTTCGAGAGTG-3’M....

Báo cáo khoa học: The crystal structure of a xyloglucan-speciﬁc endo-b-1,4glucanase from Geotrichum sp. M128 xyloglucanase reveals a key amino acid residue for substrate speciﬁcity potx

... at both ends. In the case of Xgh7 4A, the active cleft is open. Although a precise anal-ysis of the mode of action of Xgh7 4A has not beenperformed, Xgh7 4A appears to be an endoglucanasebecause ... were calcu-lated from a plot of initial reaction rates versus substrateconcentration using Prism (GraphPad Software, San Diego,CA, USA). One unit was deﬁned as the amount of enzymethat released ... XEG. The wild-type XEG cleaved the oligoxyloglucan at a speciﬁcsite; the Y457G variant cleaved the same substrate, but at various sites.Together, the absence of a loop in the cleft and the presence...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... picolinicacid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzymestands at a branch point of the tryptophan to NAD pathway, and deter-mines the ﬁnal fate of the amino acid, i.e. transformation ... shown assticks; the Zn2+ion and the solvent molecule bridging the metalcentre to the ligand are shown as magenta and cyan spheres,respectively.S. Garavaglia et al. Crystal structure of human ... (Grenoble,France). An X-ray ﬂuorescence scan performed on the hA-CMSD crystals using the same beamline, clearly indicated the presence of a Zn metal ion bound to the enzyme. Anal-ysis of the diffraction data...

Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

... Poznanskaya, A. A., Yakusheva, M.I. & Yakovlev, V .A. (1977)Study of the mechanism of action of adenosylcobalamin-depen-dent glycerol dehydratase from Aerobacter aerogenes. II. The inactivation ... primers5¢-CATATGCAACAGACAACCCAAATTCAGCCC-3¢and 5¢-AGATCTTATCACTCCCTTACTAAGTCGATG-3¢for the b subunit and 5¢-CATATGAGCGAGAAAACCATGCGCGTGCAG-3¢ and 5¢-AGATCTTAGCTTCCTTTACGCAGCTTATGC-3¢ for the c subunit. The segmentswere ... HindIII and BglII to yield pUSI2E (a G). DNAsegments encoding the b and c subunits of glyceroldehydratase were ampliﬁed by PCR using pairs of primers5¢-CATATGCAACAGACAACCCAAATTCAGCCC-3¢and...

Báo cáo khoa học: The crystal structure of NlpI A prokaryotic tetratricopeptide repeat protein with a globular fold potx

... 5¢-aataatccatggggagtaatacttcctggcgtaaaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢.3-TPR NlpI primers (residues 62–197) were 5¢-aataatccatgggggcacagcttttatatgagcgcggag-3¢ and 5¢-aataatggatcctcactgttccttatccgatttttcgaagtgc-3¢. ... for the sharp curvature of the array andreduced superhelical pitch, leading to the formation of a globular structure. Helix a Residues Deviationb,c A ˚AB pair W°cAB W°cAA¢Rotationd(AB) (A B¢)°Helix ... HelsinginSanomat Centennial Foundation (Finland). Data forthis study were measured at beamline X12C of the National Synchrotron Light Source. We are gratefulfor the assistance of Dr Anand Saxena in the...

Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx

... [1–4].Mammalian annexins A1 [5], A5 , and A 6 [6], as well asplant annexins from Medicago sativa [7] and Arabidop-sis thaliana [8,9], have been implicated in oxidative stressresponse. In particular, ... annexin Anx24(Ca32), but signiﬁcant diﬀerencesare seen when compared to the structure of nonplantannexins. A comparison with the structure of the mamma-lian annexin AnxA5 indicates that canonical ... the globular structure of Anx(Gh1), unlike that of mammalian annexins,clearly shows separation of t he two modules ( I/IV and II/ III) leading to greater accessibility of the intermodularspace...

Báo cáo khoa học: The crystal structure of human WD40 repeat-containing peptidylprolyl isomerase (PPWD1) pdf

... andcatalysis of isomerization for the standard substrate suc-AAPF-pNA. Black and red spectra of the Ala residues in the peptide are obtained from the uncatalyzed and catalyzed reaction, respectively. Acceleration ... biophysical assays. Using a colorimetriccoupled assay against succinyl-Ala-Ala-Pro-Phe-p-nit-roaniline (suc-AAPF-pNA), a well-characterized CypAsubstrate, we found that the isomerase domain of PPWD1 ... N, Kawarabayasi Y,Ishikawa Ki & Tabata S (1994) Prediction of the coding sequences of unidentiﬁed human genes. II. The coding sequences of 40 new genes (KIAA0041–KIAA0080) deduced by analysis...

Báo cáo khoa học: The crystal structure of the ring-hydroxylating dioxygenase from Sphingomonas CHY-1 pot

... and catalytic domains of the a subunit.In the heterohexamer, the Rieske domain interactswith the base of the adjacent b subunit and the cata-lytic domain of the adjacent a subunit. Most of the ... andcarries the totally conserved amino acids Gly a 354,Glu a 357, Asp a 359 and Asn a 363, which are part of a far-reaching hydrogen network surrounding the catalytic center, as well as Asp a 360, ... loops LI and LII are shown at the entrance of the catalytic pocket, as well as, the highly conserved helices, aca 10 and aca11. The ﬁgure was drawn using the programsMOLSCRIPT [37]andRASTER 3D...

Báo cáo khoa học: The crystal structure of Thermoactinomyces vulgaris R-47 a-amylase II (TVA II) complexed with transglycosylated product potx

... ÔdamÕ of the cleft.These four loops of TVA II are superimposed on those of a- amylase (Taka-amylase A) from Aspergillus oryzae (PDBcode, 7TAA) [31] and cyclodextrin glucanotransferase(CGTase) from ... into the active cleft compared to Loop II in TVA II (Fig. 5B). Inmost a- amylases, loop II makes a large bank in the activecleft, as in CGTase and TAA. In contrast, loop II of TVA II is short and ... produces a- amylase II (TVA II) as an intracellular enzyme [4]. TVA II hydrolyzes a- 1,4-glucosidic linkages of starch like other a- amylasefamily enzymes to produce mainly maltose. In additionto a- amylase...

Báo cáo khoa học: The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis Implications for the substrate activation mechanism of this enzyme ppt

... observations. Acta Crystallogr A3 4, 517–525.34 Vaguine AA, Richelle J & Wodak SJ (1999) sfcheck :a uniﬁed set of procedures for evaluating the quality of macromolecular structure- factor data and ... decarboxylated to yield the central interme-diate of ThDP catalysis, the a- carbanion ⁄ enamine(III). Protonation of III yields hydroxyethyl-ThDP(IV), and the release of the second product acetalde-hyde ... described the structural consequences of substrate activation on the basis of the crystal struc-ture of pyruvamide-activated ScPDC compared to that of ScPDC crystallized in the absence of any effectors[9],...

Xem thêm