... Journal compilation ª 2006 FEBS
The equinatoxin N-terminus is transferred across planar
lipid membranes and helps to stabilize the transmembrane
pore
Katarina Kristan
1
, Gabriella Viero
2
, Peter ... contacts is between the monomers,
which should stabilize the toroidal pore [19]. Thus, the
purpose of this work was to gain further insight into
th...
... pre-
cursor, they are probably located in the cytosol. There-
fore, aspartyl aminopeptidase activity may be related
to the metabolism of cytosolic peptides, particularly
during the final step in their ... belong to the M18 family of metal-
loprotease, comprise 12 identical subunits, and the
activity of these enzymes is inhibited by EDTA [4,5].
These two enzymes may be related...
... exocytosis [12,16]. This exocy-
tosis reaction is important for the host defense ability
to engulf and kill invading microbes; hemolymph
coagulation prevents the leakage of hemolymph and
the spread ... interacts with G protein and receptor-
independently induces the exocytosis of mast cells [22].
Previously, we found that mastoparan is able to induce
the exocytosis of...
... antibodies.
Immunohistochemistry
Mice were raised and maintained according to governmen-
tal and institutional care instructions. Immunohistochemis-
try was carried out on 14 lm horizontal sections, and
fluorescence ... mammals to detect and discriminate between thou-
sands of different odorant molecules is governed by the diverse array of
olfactory receptors expressed by olfac...
... c and the complete a-helical C-terminus
as held within the top of (ab)
3
. The rotary axis z was aligned
along the main axis of the shaft. The ‘bearing’ included a
total of 138 residues from the ... on the two groups of four
carbon atoms each. The first group included the C
a
atoms of
cK18, cI19, cT20 and cK21, and the second group C
a
atoms of cD233, cN234, cA235...
... that the
regioselectivity and enantioselectivity of epoxidation are
cytochrome P450 dependent [37,38]. For CYP77A4, the
requirement of unsaturation, in the cis configuration,
together with the ... plants, and only a few biological activities
have been attributed to them. The discovery of such
activities in plants might help to understand the physi-
ological role of CYP77A4....
... inserted into
its own b-sheet A to form a latent conformer [16].
However, this is unlikely as the latent conformer of the
serpins is unable to form polymers [17,18]. Other charac-
teristics of the latent ... [1,8]. This
contrasts with other conditions such as Parkinson’s and
Huntington’s disease in which the mutant proteins form
disordered aggregates within the cytoplasm [...
... of cofactors and adrenal androgen
The absence of stimulatory activity of AR45 might be
linked to poor coactivator recruitment, due to the
absence of the NTD. This might be overcome by the
overexpression ... found in the regulatory regions of
their target genes [14]. Due to the involvement of
these receptors in several major diseases, extensive
research has been carried out...
... APP
695
-expressing cells is due
to the fact that the epitope for the antibody against
rodent Ab is not present in human APP (Table 1),
whereas the absence of this band in the APP KO
extract confirms the specificity ... samples
(Fig. 1C). As the band migrating at 94 kDa was rec-
ognized by antibodies directed to both the ectodomain
and the C-terminus, this band app...
... Sp1 and Sp3 are
the major transcription factors that bind to GC-boxes
1 and 2 in the NOX4 promoter.
In vivo binding of Sp1 and Sp3 to GC-boxes 1
and 2 in the NOX4 promoter
To examine whether ... Sp1 and Sp3 to GC-boxes 1 and 2 in
the NOX4 promoter
The GC-box is known to bind Sp ⁄ Klf transcription
factors, especially Sp1, Sp3 and Sp4 [14,15]. To exam-
ine...