Báo cáo khoa học: High-resolution NMR studies of the zinc-binding site of the Alzheimer’s amyloid b-peptide pdf

Báo cáo khoa học: P NMR studies of energy metabolism in xanthosine5¢-monophosphate overproducing Corynebacterium ammoniagenes pot

... ratios(glu/glc) in the medium were adjusted to a molar ratio of 0.31. The 31P NMR illustrated the two distinct phases of the cellular energetic status due to the availability of the sub-strates from the medium. ... supplementation of the carbon skeleton and the tricarboxylic acid (TCA)cycle for maximization of ATP production was discussed.However, direct estimation of contribution of these twometabolic pathways in the ... important because the nucleotidebiosynthesis requires high levels of ATP [1,2]. Difﬁculties inobtaining the energy metabolism proﬁle from living cellsare details of the regulatory process of nucleotide...

Tài liệu Báo cáo khoa học: Solution NMR structure of an immunodominant epitope of myelin basic protein Conformational dependence on environment of an intrinsically unstructured protein doc

... conditions that would allow high-resolution NMR studies of MBP in an environ-ment most closely mimicking the native myelin sheath.Although there have been previous NMR studies of other MBP-derived polypeptides ... shows the electrostaticsurface charge of residues P5¢ to P16¢ of the proposedstructures of FF2from two orthogonal view angles. The partitioning of charges onto opposing faces of the helix further ... outside the hydrophobic interior of the micelles. These molecules act locally as strong signal-relaxing agents, causing a broadening proportional to the inverse of the average of the distance to the...

Tài liệu Báo cáo khoa học: Solution NMR structure of ﬁve representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity doc

... bondbetween the hydrogen of OH9 (HO9) and the oxygen of OH7 (OH7) which can contribute to the stabilization of the C7–C9 region of the macrolide.Structural analysis of nystatin A1 The spectral ... However, for the C1–C7 segment, the two hydroxyl groups on C3 and C5 are, respectively,hydrogen bonded with the CO groups of C1 and C7 in the majority of the models. For the C10–C13 segment, the hydroxyl ... the ﬁve macrolides,is the D-mycosamine glycosyl moities always located on the opposite side of the polyene plane.Noteworthy, these common structural features areconserved, regardless of the...

Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deﬁciency and hypervitaminosis pot

... vitamin A-deﬁcient rats. In the liver of rats with hypervitaminosis the results showed a decrease intheamountofp53andanincreaseofthec-Jun .The gureshowsthat the expression of p53 is time course dependent. ... wasanalyzed by the Levene test; in those cases in which the variances were unequal, the data were adequatelytransformed before the ANOVA. The null hypothesis wasaccepted for all the values of these ... 7)consists of the direct regulation that exerts the retinoic acidon c-Jun expression23. The increased levels of c-Jun induce apositive effect in the expression of the cyclin D1 gene and the down-regulation...

Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

... iden-tiﬁed as the major and the minor forms of the holo-ACP structure, on the basis of their percentage contri-butions (65% and 35%, respectively) to the overallstructure of the protein. The structures ... estimated on the basis of the linear regression of the fraction number of the molecular mass markers cytochrome c (CyC), carbonic anhydrase (CA), andBSA. From the calibration curve of the sucrose ... in the survival of the malarialparasite. The essential role of fatty acids and lipids incell growth and differentiation, and the occurrence of a different type (type I) of fatty acid biosyntheticpathway...

Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf

... the projection of the tilt of the z axis on the x¢,y¢ plane (defined direction of tilt of z and the x¢ axis), and j % a + c defines the location of the rhombic (x, y) axes. The orientation of the ... in the 1H NMR data of Pe HbO2. The static structure of neither WT nor rWT metMb would allow the TyrE7 ringreorientation without significant distortion of the hemepocket.Comparison to other ... consistent with the % 10° tilt of the Fe-CN vector from the heme normal to avoid van der waalsoverlap with the Tyr32(B10) Og. The location of the rhombic axes, j % 50 ± 10°,isinagreementwiththeexpectations...

Báo cáo khoa học: An NMR study of the interaction between the human copper(I) chaperone and the second and ﬁfth metal-binding domains of the Menkes protein pot

... the ATP7B protein that the second metal-binding domain constitutes the pre-ferred one for the uptake of the ﬁrst metal ion by the ATPase from the chaperone, as a result of the speciﬁc-ity of ... preferentialinteraction of the chaperone with the second domainseems unlikely also in the case of ATP7B. The selectiv-ity, if any, for the interaction of one of the six metal-binding domains with the chaperone ... by the behaviour of the NMR signals along titrations. Con-sequently, we can observe experimentally only the copper(I) exchange process. The thermodynamic con-tribution to the formation of the...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... mice [28]. The instability of DA at the neutral pH of the cytosol causes the forma-tion of toxic aminochromes, and these species are notformed when DA is protonated at the acidic pH of the storage ... counter. Values are the mean of six replicates. The speciﬁc uptake was determined bysubtracting the uptake counts in the absence of inhibitorfrom the uptake counts in the presence of cocaine or S-cita-lopram, ... Co-expression of p25aand the dopamine transporter in HEK-293-MSR cells sensitized them to the toxicity of extracellular dopamine. Neuronal expression of p25a thusholds the potential to sensitize the...

Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx

... we focus on the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obes-ity and related metabolic diseases, and discuss whetherthese ANGPTLs could be targets for the preventionand treatment of these conditions.Suppression ... adiposetissue of ANGPTL2 null mice compared with that of wild-type mice [15]. These results raise the possibilitythat blocking ANGPTL2 signaling simultaneously sup-presses the expression of other inﬂammatory ... it is predicted that the suppression of ANGPTL2 signaling has few sideeffects. Therefore, for these reasons, we consider that the suppression of ANGPTL2 signaling as a therapeu-tic strategy...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... observation). In the ternarycomplex, the histone H3 peptide binds in a deep chan-nel that divides a pair of acidic lobes, one of which iscomposed of residues from the SET-I region and the other of residues ... present. This is because the con-struct used to determine the structure of the MLL1SET domain lacks the evolutionarily conserved Winmotif in the region ﬂanking the N-terminus of the SETdomain [26], ... (green) bound to the central opening of WDR5 (PDB code3EG6). The position of the conserved Arg 3765 is indicated. On the left, a stick representation is used to show the position of the MLL1Win...

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