Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

... Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique Juana ... inﬂuence the efﬁcacy of interference RNA in vivo, for example, the length of target mRNA, the length and concentration of dsRNA, the region of homology between the dsRNA and the target, as well as other ... expression of L. schmitti CHH gene The size and expression of the CHH mRNA in differ-ent tissues were determined by northern blot analysis of total RNA isolated from eyestalk, muscle and stom-ach....

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... of the 53 kDa spot (5.3) was identical to a pI value calcu-lated from the amino acid sequence of GmPDIL-3a, and the isoelectric point of the 57 kDa spot (5.1) wasconsistent with that from the ... organelle and membrane proteins are synthe-sized and folded with the assistance of molecular chap-erones and other folding factors in the endoplasmicreticulum (ER). In many cases, the process of ... GmPDIL-3a and GmPDIL-3b were detected at a density of 1.21 gÆmL)1in the presence of MgCl2. In the presence of EDTA,which releases ribosomes from the rough ER [24], the peaks of GmPDIL-3a and GmPDIL-3b...

Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulﬁde isomerases as molecular chaperones for seed storage proteins doc

... cloned and characterized the cDNAs of GmPDIL-1 and GmPDIL-2 as members of the PDIfamily. The amino acid sequences and putative domainstructures of GmPDIL-1 and GmPDIL-2 were similarto each other, ... b-conglycinin during the course of the folding process of these proteins.Results Cloning and expression of GmPDIL-1 and GmPDIL-2To clone the soybean ortholog of ArabidopsisPDI-like 1-1 and PDI-like ... formation in the ER and may cause the accumulation of unfolded proglycinin. As the active sites of the PDI family proteins are reduced in the ER in the presence of dithiothreitol, neither GmP-DIL-1...

Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc

... parameters of CYP719A3, the amount of nandinine produced was estima-ted by using the calibration curve of (S)-scoulerine (pmolversus peak area) and the absorption ratio of (S)-scoulerine and (S)-nandinine ... prepared from coptisine by the samemanner as (R,S)-tetrahydroberberine.Ampliﬁcation of cytochrome P450 cDNA fragments from single-stranded cDNAs of cultured E. californica cellsSingle-stranded cDNAs ... samples and open circles are untreated controls. The relative expression level shows the values standardized by that of control (0 h sample) as 1. The error bar indicates the standard error of the...

Báo cáo khoa học: Identiﬁcation and characterization of the metal ion-dependent L-alanoyl-D-glutamate peptidase encoded by bacteriophage T5 pdf

... strains B, Z85 and BL21(DE3) and bacteriophageT5+were taken from the collection of the Laboratory of Molecular Microbiology of the Institute of Biochemistry and Physiology of Microorganisms ... TritonX-100), and the chelators were added. The reaction was ini-tiated by the addition of a standard amount of the enzyme(15 ng mL)1).Extraction of peptidoglycanPeptidoglycan was extracted by Streshinskaya’s ... diverse pH and ionicstrength optima. The spectrum of antibacterial action of endolysins isdetermined by the enzyme type, composition of the cellwall components of the target bacterium, and conﬁgu-ration...

Báo cáo Y học: Molecular cloning and characterization of isomultiﬂorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt

... [15],suggesting the existence of additional OSCs. As part of aneffort to understand the molecular mechanism of the triterpene cyclization reactions, as well as the molecular evolution of these proteins ... mL·min21. The contents of bryonolicacid and total sterols were calculated from the peak area of their trimethylsilylated products relative to that of the internal standard. Sterol content was the ... pYES2-LcIMS1 in which the ORF of the cDNA was ligated to the GAL1 promoter in the senseorientation. The nucleotide sequence of the inserted DNAwas conﬁrmed by sequencing. The ERG7-deﬁcient yeaststrain...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... expression of T-bet has beenseen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse ... CD4+CD25+Foxp3+Tregor whetherthey induce the production of these cytokines from another population of cells [20].To date, our knowledge about the different types of Th and Tregresponses relates ... and, in the case of t-bet and foxp3, by exploiting the conservation of synteny between the human and zebraﬁsh genomes. Chro-mosomes within the zebraﬁsh genome database weresearched by basic...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... 40G14,9E14, 51H3, and 6H19. The BAC DNA was isolated, and the presence of the SmSmad1B sequence was conﬁrmed by PCR and by sequencing the BAC DNA. Exon–intron siteswere located by aligning the cDNA sequence ... R-Smads (Smads 1, 5 and 8),as the functions of these Smads in other organisms arehighly redundant. Future analysis of the target genesactivated by the BMP-related Smads from schisto-somes ... 4075–4093 ª 2007 The Authors Journal compilation ª 2007 FEBS 4087enhanced the interaction of SmSmad2 and SmSmad4 and resulted in the formation of a stable and functional Smad complex [6,7]. The above...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... physiologicalelectron acceptor of the enzyme. The enzyme was notoxidized by the heterodisulﬁde of coenzyme M and coenzyme B, or by the homodisulﬁdes of these twocoenzymes. Also potassium trithionate and sodium ... amounts of th e 16-kDa c-type cytochrome. The Fig. 2. Genomic o rganization of the genes e ncoding the subunits of the Hme complex from A. fulgidus. ThegenenamesannotatedbyTIGRaregivenabove the ... Further studies are necessary toelucidate the nature of the extractable heme present in thisenzyme. The oxidation of the heme groups of the enzyme by various compounds was tested to identify the...

Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... content in their latex; and (2) the pres-ence of other cysteine proteinases or isoforms in the latex. To investigate these two hypotheses, the proteinconcentration per milligram of dried latex, ... research was provided by agrant to T. Kyndt from the ‘Institute for the Promo-tion of Innovation through Science and Technology inFlanders (IWT-Vlaanderen)’ and by FWO-Vlaanderen(project number ... the other sequences. The MP tree reveals threemajor clusters. Cluster I combines the ﬁve chymo-papain isoforms of C. papaya with VXH-C, VS-B,CC-III and CC23. These results conﬁrm the hypothesisof...

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