Báo cáo khoa học: Leishmania infantum LeIF protein is an ATP-dependent RNA helicase and an eIF4A-like factor that inhibits translation in yeast docx

... protein is an ATP-dependent RNA helicase and an eIF4A-like factor that inhibits translation in yeast Mourad Barhoumi1, N. K. Tanner2, Josette Banroques2,3, Patrick Linder2 and Ikram Guizani11 ... puriﬁed, recombinant, LeIF protein from Leishma-nia infantum, and we demonstrate that it is an RNA- dependent ATPase and an ATP-dependent RNA helicase. Sequence alignments show that LeIF is closelyrelated ... We show that a His-tagged, recombinant, LeIF protein of Leishmania infantum, which was puri-ﬁed from Escherichia coli, is both an RNA- dependent ATPase and an ATP-dependent RNA helicase in vitro,...

Báo cáo khoa học: Tetracysteine-tagged prion protein allows discrimination between the native and converted forms pptx

... ﬂuorescent protein, to the protein of interest[10]. Fluorescent protein domains may interfere with protein folding or ligand binding and, more particularly,with protein packing into ordered arrays. ... RE, Gross LA, Martin BR,Walkup GK, Yao Y, Llopis J & Tsien RY (2002) Newbiarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biologicalapplications. ... that express TCC (Bb and Cb) and TCN (Bc and Cc), FlAsH and CrAsH selectively stain thecell surface, whereas there is no staining ofthe cells expressing mPrP (Ba and Ca) protein. J. Gasˇpersˇicˇet...

Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx

... potentials that are indistinguish-able from that of the mother cell. This process is cen-tral to development, as well as to the maintenance ofadult tissues in complex and long-lived organisms.Self-renewal ... roles in RNA processing, including the splic-ing of alternative exons, mRNA stability, mRNA localization, and internalribosome entry site-dependent translation. Although it has been reported that ... Poly-pyrimidine tract-binding protein promotes insulinsecretory granule biogenesis. Nat Cell Biol 6, 207–214.10 Kosinski PA, Laughlin J, Singh K & Covey LR (2003)A complex containing polypyrimidine...

Báo cáo khoa học: Cardiac ankyrin repeat protein is a marker of skeletal muscle pathological remodelling pot

... cardiac ankyrin repeat protein, is up-regulatedduring wound healing and induces angiogenesis in experimental granulation tissue. Am J Pathol 166 , 303–312.53 Grounds MD & Torrisi J (2004) Anti-TNFalpha ... arole. In particular, cardiac ankyrin repeat protein (CARP) was suggested to be involved in these pro-cesses. CARP, together with ankyrin repeat domain-containing protein 2 (Ankrd2) and diabetes-relatedankyrin ... par-ticipates in or represents an attempt to resist the unre-lenting muscle degeneration is an important issue.It is of interest that CARP is the only protein show-ing a variation of proﬁle between transient...

Báo cáo khoa học: The optimization of protein secondary structure determination with infrared and circular dichroism spectra docx

... scaling factor determined by Fourier self-deconvolving the spectra, deter-mining the tyrosine band a rea ratios at 1518 cmÀ1,andthenusing this as the scaling factor for subtracting the originalspectra ... data points in order to bring the minimum to zero.Combination of CD and IR spectraTo analyze combined C D and IR data, h ybrid spectra weremade by placing CD and IR data in a single array. In ... determined simultaneously.Results and DiscussionMethods for evaluating analysis performanceCross validation, as explained i n the M aterials and methods,treats each protein a s a n unknown and...

Báo cáo khoa học: Selective modulation of protein C afﬁnity for EPCR and phospholipids by Gla domain mutation pdf

... capture ligand for sEPCR and protein C is illustrated in Fig. 2C. This shows ini-tial binding of sEPCR to RCR-2 (Fig. 2C; panel 1), theaddition of increasing concentrations of protein C –showing ... apoptosis in ischemic human brain endothelium and is neuroprotec-tive. Nat Med 9, 338–342.17 Bangalore N, Drohan WN & Orthner CL (1994) Highafﬁnity binding sites for activated protein C and protein C ... prediction and analysis of endothelial cell protein C ⁄ activated protein C receptor. Protein Eng 12,833–840.30 Fukudome K & Esmon CT (1995) Molecular cloning and expression of murine and bovine...

Báo cáo khoa học: SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc pptx

... needed.Zinc is an essential metal in all eukaryotes. Zinctransporting proteins were ﬁrst reported in yeast and plants. In mammals, the ZIP superfamily is the moststudied zinc transporter. Human zip1 and ... ninetransmembrane domains (according toTaylor et al. [15] and ﬁve transmembraneprediction software packages), histidine-richmotif [(HX)n, n ¼ 3–6], LZT protein conserved motif (HEXPHE) and ... 1599SLC39A14, a LZT protein, is induced in adipogenesis and transports zincKei Tominaga1,2, Takeshi Kagata1, Yoshikazu Johmura1, Tomoaki Hishida1, Makoto Nishizuka1 and Masayoshi Imagawa11...

Báo cáo khoa học: Silencing the expression of mitochondrial acyl-CoA thioesterase I and acyl-CoA synthetase 4 inhibits hormone-induced steroidogenesis potx

... of long-chain acyl-CoA esters in signal-transduction pathways is an important issue [25]. It is known that an acyl-CoA-binding protein known alsoas DBI (diazepam-binding inhibitor) is expressed ... mitochondrial cristae [17]. In accord-ance with the postulated role of MTE-I in steroido-genesis, we detected the protein and its mRNA in theadrenal gland, ovary, testis, placenta and brain [16].Although ... action of these two proteins is the rate-limiting step in steroidogenesis [6]. Fatty acyl-CoAs bind to theacyl-CoA-binding protein, which can bind and maythereby activate PBR. This interaction favors...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... PDZproteins: Mint-3, AMPA binding protein and gluta-mate receptor interacting protein (GRIP). It wouldtherefore be interesting to determine whether this motif is also involved in the interaction ... cellstransfected with pCDNA3.1 Zeo+ and MT1/MYC (lane 1), pCDNA3.1 Zeo+ and GRASP55F (lane 2), pCDNA3.1 Zeo+ and MT1 LLY/MYC (lane 3), GRASP55F and MT1 LLY/MYC (lane 4) and GRASP55F and MT1/MYC (lane ... transiently transfected with pCDNA3.1 Zeo+ and MT1/MYC(lane 2), pCDNA3.1 Zeo+ and GRASP55F (lane 3) and MT1/MYC and GRASP55F (lane 4) were immunoprecipitated with the FLAGM2 monoclonal antibody and the...

Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx

... There is evidence that the heteromeric guanine nucleotide-binding regulatory protein (G protein) is also involved in TLR4 activation. It has been shown that LPSinduced TNFa production which can ... their inactive conforma-tion G proteins consist of a-, b- and c subunits,whereas only the a subunit is bound to GDP. GPCRare transmembrane receptor proteins, containing sevenmembrane-spanning ... dis-covered genetically as negative regulators of G -protein signalling in lower eukaryotic organisms includingAspergillus and Caenorhabditis elegans.Currently, more than 25 mammalian RGS proteinshave...

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