Báo cáo khoa học: A unique tetrameric structure of deer plasma haptoglobin – an evolutionary advantage in the Hp 2-2 phenotype with homogeneous structure pot

... Schematic drawing of tandem repeat region (B and B1) of deer and human a- chain. The most signiﬁcant feature of human a2 is that itmatches the ABC domains of a1 but with an additional insertion of ... properties.Molecular mass estimation of deer and human Hp 2-2 using SDS–PAGE and western blotWestern blot analysis using the a chain-speciﬁc mAbW1 indicated that the mAb recognizes both humanand deer a chains ... presence of a- chains was12345Fig. 2. Hemoglobin-binding patterns of deer and human plasma Hp on 7% native PAGE. Lane 1, hemoglobin only. Lanes 2, 3 and 4,human plasma of Hp 1-1, 2-1 and 2-2 phenotypes...

Tài liệu Báo cáo khoa học: A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 pptx

... ribonuclease A (13.7 kDa) serving as molecular standards (Amersham Bio-science).Analysis of the N-terminal amino-acid sequences The N-terminal amino-acid sequences of the enzymes wereanalyzed using ... product. The a1 and b1 geneswere arranged in tandem (a1 –b1) and were estimated toencode proteins with molecular masses of 55 316 and42 685 Da, respectively.On the other hand, SDS ⁄ PAGE analysis ... Tokushima, JapanDye-linked dehydrogenases (dye-DHs) catalyze the oxi-dation of various amino acids, organic acids, aminesand alcohols in the presence of artiﬁcial electron accep-tors such as 2,...

Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx

... CPT1B cDNA, we used the construct pGEMT–5¢HumanCPT1B as a template in a PCR reaction with primers DH673 (5¢-AGCTGAATTCATGGCGGAAGCTCACCAG-3¢) and DH803 (5¢-TCCACCCATGGTAGCAGAGAAGCAGCTTAAGGGTTTGGCGGA-3¢). ... andHumanCPT1Bmut–BspTI–pBSSK+ with ApaI and BspTI,taking advantage of an ApaI restriction site located in the BSSK+ polylinker and a BspTI restriction site in pigCPT1B and human CPT1B cDNAs (position +382). The fragments ... 18PigCPT1B and D28PigCPT1B). The assaywas performed at 1 mm carnitine as standard. To analyzePigE17DCPT1B and HumanD17ECPT1B mutants, the assay was performed at carnitine concentrations equal tothe...

Báo cáo khoa học: Stage speciﬁc expression of poly(malic acid)-afﬁliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx

... Donze and Picard[37]. In the case of NKA8, the forward primer was 5¢-GATGCATAATACGACTCACTATAGGGAGTGCCTTGCAAGGAGTATTG-3¢ and the reverse primer was 5¢-GCCTTCTAATACGACTCACTATAGGGAGCTCGTAATAGCTTTTGGAC-3¢, ... 5¢-GATGCATAATACGACTCACTATAGGGAAATGTCCGTCCAACAAGGAG-3¢ (forward) and 5¢-GCCTTCTAATACGACTCACTATAGGGACCACGATGATGGATGAAATG-3¢ (reverse). Both primers containedT7-polymerase promoter at the 5¢ terminus and were ... databases that would beindicative of a PMLA synthetase. Quantitative PCRrevealed that polymalatase mRNA was expressed atconsiderably lower levels in amoebae and spherulesthan in plasmodia....

Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx

... levels of both Glc1Man9GlcNAc2 and peak X were increased(Fig. 2A, B). Under these conditions, the decreasingamounts of Man9GlcNAc2 and Man5GlcNAc2 correlated with the increasing amounts of Glc1Man9GlcNAc2 ... higher than the ratio Glc1Man5Glc-NAc2/Glc1Man9GlcNAc2 obtained with the glycoproteinpattern. This indicates that, when glycoproteins bearingMan9 and Man5 are synthesized in the same cell line, ... look at the end products of processing,most of the Man5GlcNAc2 was converted into Man4Glc-NAc2, in contrast with the small amount of Man6GlcNAc2originating from the Man9GlcNAc2 species.ERAD of...

Tài liệu Báo cáo khoa học: A unique variant of streptococcal group O-antigen (C-polysaccharide) that lacks phosphocholine ppt

... of F)andtodH4.02 (H- 6a andH-6b, of A) andasthesignalatdP – 0.04 correlated todH4.13 (H- 1a and H-1b, of G)andtodH4.07 (H- 6a andH-6b, of D). The two phosphoethanolamine groups aretherefore ... tetrasaccharide-ribitol. The data shows that the AAT residue is indeed an acetamido-amino derivative and supports the postulatedrepeat as CBADE.From the combined data obtained from NMR and massspectrometry the ... represents a sub-group of biovar 1, possesses a unique variant of the C-polysaccharide found in the cell wall of all strains of Streptococcus pneumoniae and in some strains of S. mitis.This new variant...

Báo cáo khoa học: A unique vertebrate histone H1-related protamine-like protein results in an unusual sperm chromatin organization pot

... speciﬁcityresides in the intrinsically disordered domains of theirN- and C-terminal tails. In the case of the PL-I pro-teins, there is a substantial increase of arginine withinthese regions (see ... of the sperm chromatin organization based on the information obtained from (A) and (B). As seen in (A) , DNA bundles of approximately 650 ± 100 A ˚appear to be intertwined (giving the appearance ... organize chromatin in parallel DNA bundles of different thickness in a quite distinct arrangement that is reminiscent of the chromatin organizat ion of those organisms that contain protamines(but...

Báo cáo khoa học: A unique binding epitope for salvinorin A, a non-nitrogenous kappa opioid receptor agonist docx

... comparative analysis [1]. Regard-less, models were developed based on overlap of the furan ring of salvinorin A and the phenyl ring of the arylacetamides. The centroids of the aromatic ringsand ... they may share a commonpocket in stabilizing the binding of salvinorin A to the KOP. The remaining residues proposed in Fig. 2 do notappear to have a signiﬁcant impact on salvinorin A binding. ... currentbinding-site models and to gain insight into the design of additional salvinorin A analogues. A tentative modelis also proposed to explain previous and new data onsalvinorin A binding to the opioid...

Tài liệu Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics ppt

... proﬁling between cancer and normal cells enables identiﬁcation of aberrant glycosylation in cancer [indicated as a red triangle in (B) and (C)] as an alteration in lectin signal pattern. According ... antibodies, and their glycan alterationwas examined by a lectin microarray. Finally, they were analyzed by multi-stage tandem MS.AbbreviationsAAL, Aleuria aurantia lectin; AFP, a- fetoprotein; GGDB, ... knowledge of human glycosylation,glycan structural analysis systems, the bioinformaticscapability and the databases that we have developedover the years, and animal models of aberrant glyco-sylation...

Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx

... 5¢-AAGTAGGCGGAGTATCGAAC-3¢ (sense) and5¢-GAAAAAACGCGATCCTACTT-3¢ (antisense). Primersfor unmethylated DNA were: 5¢-GAAGTAGGTGGAGTATTGAAT-3¢ (sense) and 5¢-CAAAAAAACACAATCCTACTT-3¢ (antisense).Caspase 3 activityCells ... to invasiveness in cancer. Cancer Res 61, 349 3–3 500.30 Giannelli G, Bergamini C, Fransvea E, Sgarra C &Antonaci S (2005) Laminin-5 with transforming growthfactor-beta1 induces epithelial ... occludin (OccWT) and variant occludin in apoptosis and invasion, as determined by assay, werevealed that exon 9 played a major role in the induc-tion of mitochondria-mediated apoptosis and the reduction...

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