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Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot

Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot

Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot

... that of the wild-type and withan optimum pH of 6.5. A shift of the pH profile of about + 0.5–1.0 pH units and an optimum pH similarto that of the wild-type was observed for the H334G mutant in the ... Characterization of PLP phosphate in wild-type CcStP and the H334G mutant using31P-NMR. (A) Spectra of wild-type CcStP and the H334G mutant acquired atpH 7.25, and with the number of recordedscans and ... mightoccupy the position vacated in the H334A mutant through removal of the imidazole group of the His,thereby effectively replacing the function of the origi-nal side chain in catalysis by the mutant...
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Báo cáo khoa học: Rotenone inhibits mammalian cell proliferation by inhibiting microtubule assembly through tubulin binding Pallavi Srivastava and Dulal Panda ppt

Báo cáo khoa học: Rotenone inhibits mammalian cell proliferation by inhibiting microtubule assembly through tubulin binding Pallavi Srivastava and Dulal Panda ppt

... F0, Fc and DFmaxrepresent the fluorescence intensity of tubulin in the absence of rotenone, the fluorescence intensity of tubulin in the presence of different concentrations of ligand and the maximum ... avariety of cell types and several mechanisms, such asactivation of the Jun N-terminal kinase pathway,involvement of the caspase activated DNAase, the redistribution of p53 and the activation of ... several morphological and biochemical changes in the cell. One of thesechanges is the exposure of phosphtidylserine on the surface of the cell membrane during the early stage of apoptosis. Annexin...
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Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot

Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot

... special function of E393 can beproposed on the basis of the results presented here.Two of the mutants (D217E and E221Q) showed noactivity in any of the assays. These mutants (and wild-type) ... reactions at a single active site [1]. One of the main purposes of this study was to try todissect the two reactions by mutagenesis of active site residues, expecting to find mutants of the E. coli enzymein ... favoured by the near irreversi-bility of the conversion of the 2-ketoacid to TS2. However the reaction is inefficient due to slow conversion of TS2 to TS1.Mutagenesis of the active site of E. coli...
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Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

... determined at pH 5 and 0.05% SDS, and DO activity at pH 7 and 0.02% SDS.Fig. 3. Dependence of the TH activity of extracts of R3 and the two mutant strains on the presence of cofactor,L-dopa. ... but the differentiation between these two types of enzymeis not clear [4]. Looking at the sequences of the two enzymes, both show absolute conservation of the histi-dine residues of the CuA and ... be part of the defense sys-tem against wounding and infection by the pathogen[16]. Thus, the timing and location of melanizationseems to be essential for the prevalence of one of thesetwo...
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Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot

Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot

... trunca-tions, the availability of the Tyr190 mutants makes itfeasible to conduct cell-based studies of the effects of the stability of the acetylated enzyme intermediate on the N-acetylation of aromatic ... hypothesized that modulation of the cata-lytic potential of the catalytic triad might be accessible through point site mutations of the proximal residue,Tyr190, which is highly conserved and ... incubation of rabbit liver NAT with [2-3H] acetyl-coenzyme A(AcCoA) in the absence of amine [21] and the active site Cys68 or Cys69 has been further identified through thiol-specific modification and site- directed...
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Báo cáo khoa học: Probing the unfolding region of ribonuclease A by site-directed mutagenesis potx

Báo cáo khoa học: Probing the unfolding region of ribonuclease A by site-directed mutagenesis potx

... L35S/F46Y, and K31A/R33S/F46Y) and studied the effect of the mutations on the thermodynamic and kinetic stability. The similarity of the impact on both the thermodynamic and kinetic stabilitiessuggests ... stabilized by a network of hydrogen bonds between the side chain of Arg33 and the backbone of Met13 and Arg10(three hydrogen bonds) and between the side chain of Arg10 and the backbone of Arg33 (one hydrogen ... Hydroge n b ondsbetween the side ch ains o f Arg33 and the backbone of Met13 and Arg10 and the hyd rogen bo nd be tween t he side ch ain of Arg10 and the backbone of Arg33. The hydrophobic residues...
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Báo cáo khoa học: Probing plasma clearance of the thrombin–antithrombin complex with a monoclonal antibody against the putative serpin–enzyme complex receptor-binding site docx

Báo cáo khoa học: Probing plasma clearance of the thrombin–antithrombin complex with a monoclonal antibody against the putative serpin–enzyme complex receptor-binding site docx

... reduction of the Cys residue at position 430 of AT, it is unlikely that the epitope is at the very C-terminus of the peptide. The results therefore suggested that residues 411 and 416 are part of the ... toform the critical hydrogen bond of the catalytic triad – abond that is a prerequisite for cleavage of the acylintermediate that links the protease to the serpin. Moreover, the distortion of the ... demonstrated that the rate of SEC clearance is 10–50 times faster than that of the corresponding free inhibitor [47]. In the case of T–AT, the t½for the elimination of the complex from the circulation...
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Báo cáo khoa học: Probing the determinants of coenzyme specificity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis potx

Báo cáo khoa học: Probing the determinants of coenzyme specificity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis potx

... requires further structuralinsight is, on the one hand, the widespread use of Aspat the P7 position in many naturally occurringNADPH-dependent GDHs and, on the other hand, the failure of the E243D ... attainedfor the wild-type enzyme, nor any of the mutants.Accordingly, the measurements in the presence of oxo-glutarate were the most useful for overall comparison. The wild-type Kdfor NADH in the ... Kdfor NADH in the presence of oxo-glutarate was 0.72 lm and each of the mutants showed the anticipated weakening of NADH binding, to the extent, in the cases of E243K and E243R, that measur-able...
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Tài liệu Báo cáo khoa học: Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis docx

Tài liệu Báo cáo khoa học: Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis docx

... position of the substrate was determinedon the basis of the possible binding sites identified by the Site Finder function in moe, as well as the relativeposition of the indole substrate in the crystal ... nmolÆmin)1Æmg)1protein. On the other hand, the 24DMA activity of the V205A mutant wasreduced to 0.1 nmolÆmin)1Æmg)1protein (Table 2). The 24DMA conversion products from the I248L,V205A and WT enzymes had the ... reported which of the five genes control the substrate specificity of the AtdA enzyme. The objective of this study was to identify and probe the residues determining the activity as well as the sub-strate...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Probing the lexicon in evaluating commercial MT systems Martin" pot

... to judge the information content of the translations, the translation quality, and the user-friendliness. The list of criteria for self evaluation consisted of technical, linguistic and ergonomic ... used for comparing the sizes of lexicons since the number of error tokens is given rather than the number of error types. Furthermore it is questionable if a running text of 5000 words says ... derived from the Mannheim cor- pus of the "Institut fiir deutsche Sprache" and for English were computed from the Cobuild corpus of the University of Birmingham. Looking at the fre-...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinBT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘI