... requires further structuralinsight is, on the one hand, the widespread use of Aspat the P7 position in many naturally occurringNADPH-dependent GDHs and, on the other hand, the failure of the E243D ... attainedfor the wild-type enzyme, nor any of the mutants.Accordingly, the measurements in the presence of oxo-glutarate were the most useful for overall comparison. The wild-type Kdfor NADH in the ... Kdfor NADH in the presence of oxo-glutarate was 0.72 lm and each of the mutants showed the anticipated weakening of NADH binding, to the extent, in the cases of E243K and E243R, that measur-able...