... of Bacillus anthracis
FEBS Journal 275 (2008) 6237–6247 ª 2008 The Authors Journal compilation ª 2008 FEBS 6247
Identification, characterization and activation mechanism
of a tyrosine kinase of ... as Walker A, A and B,
with some exceptions [4]. The majority of the bacterial
tyrosine kinases possess a transmembrane domain and
an intracellular catalytic dom...
... I-D-AEA-K R-DD-QNTIVK-VGATAASASA 122
A
Active site
R101
B
N
R
I/M
HvTrxh1
HvTrxh2
Ta
a
Os
a
Os
b
Td
Ta
b
Ta
c
Ta
d
Hv
a
Sc
Lp
Hb
Os
c
C
K
N
A
Zm
Os
e
Os
d
Lc
Pc
Ata
Atb
Atc
Atd
Ate
Atf
Atg
Bna
Bo
Bnb
Bra
Brb
Pm
Fe
Cm
Nta
Pt
Ath
Ati
Rc
Ntb
Ntc
Pp
Psa
Psb
Psc
Psd
*
Fig. ... purified
protein was missing methionine and alanine from the
N-terminus. This was confirmed by the N-terminal
se...
... proenzyme
of phenol oxidase A
1
of Drosophila melanogaster. Proc. Natl Acad.
Sci. USA 92, 7769–7773.
13. Kawabata, T., Yasuhara, Y., Ochiai, M., Matsuura, S. & Ashida,
M. (1995) Molecular cloning of ... bp of the 5¢
untranslated regions and the 3¢ untranslated regions
containing polyadenylation signals (AATAAA) at three
positions and the poly (A) -tails. The criteria for a...
... CATCGTCATGTTTGAACC
RTWF5. 1a/ 3¢ PFIKPR
a
CCTGGGTTTAATAAATGG
Actin ActF(WF) AALVVD TCGCTGCCCTCGTTGTTGAC
ActR(WF) VLLTEAP
a
GGAGCCTCGGTCAGCAGGA
a
Primer based on complement.
Table 2. Properties of ... estimate the net charge K and R were assumed to have a value of + 1, H of + 1/2, D and E of )1,
and C-terminal amidation was counted as an additional +1.
Label Residues Amin...
... DR2:
5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCG
TAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAA
GGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGG
TCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCAAGGT
CATGACCTCG-3¢. One strand of each oligonucleotide ... bp was
produced by PCR amplification with TOPO 2.1-SmNR1 as
a template (forward primer: 5¢-ATTTCAGAAGTTGAAC
AAACACAC-3¢, reverse primer: 5¢-AAGATGGTATT
GAAGATGATGGTTGA-3...
... 25–29.
12 Kawakami R, Sakuraba H, Kamohara S, Goda S,
Kawarabayasi Y & Ohshima T (2004) Oxidative stress
response in an anaerobic hyperthermophilic archaeon:
presence of a functional peroxiredoxin ... the first of all Prxs analysed
so far in archaea that has only one cysteine residue in
the sequence.
Transcriptional analysis of bcp2 under oxidative
stress and characterization...