Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx
Báo cáo khoa học: Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme pptx
... compilation ª 2009 FEBS Functional role of fumarate site Glu59 involved in allosteric regulation and subunit–subunit interaction of human mitochondrial NAD(P)+-dependent malic enzyme Ju-Yi Hsieh1,*, ... site for human m-NAD-ME. (A) Homotetramer of human m-NAD-ME (Proein Data Bank code: 1PJ3). (B) Fumarate- binding site of m-NAD-ME. The corresponding amino acids in the fumarate- binding site, Arg67, ... of the charge and hydrogen bonding network of the Glu59 side chain on allosteric regulation of the enzyme. Charge effect of residue 59 on enzyme regulation In the structure of the wild-type enzyme, ...
- 12
- 360
- 0
Báo cáo khoa học: Functional role of the linker region in purified human P-glycoprotein pot
... ‘linker region’ of $ 75 amino acids that connects two homologous halves, each of whichcontain a transmembrane domain followed by a nucleotide-bindingdomain. To investigate the role of the linker ... activity in mutants of the human multidrug resistance protein.J Biol Chem 271, 1877–1883.31 Loo TW & Clarke DM (1999) Identification of residues in the drug-binding domain of human P-glycoprotein.Analysis ... symbol. The initial hydrolysis ratewas routinely calculated using a one-point assay at 30 minbecause linearity in the time course was confirmed until30 min within 37 lm per min of the initial rate...
- 13
- 313
- 0
Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the fibrin polymerization process pdf
... polymerization sites of fibrin monomers. Site ‘A’ (Aa17–19) is exposed in the N-terminal region of the Aa-chain by the splitting off of fibrinopeptide A[6]. Site ‘a’ is formed by amino acid residues ... polymerization of fibrin obtained from recombinant fibrinogen withhistidine substituted for arginine at Bb14. Turbidityanalysis showed an increase in lag-time and a decrease in maximum polymerization rate of ... BbArg14 is involved in another fibrin poly-merization site localized at the N-terminal fragment of the fibrin Bb-chain. This site is operational beforefibrinopeptide B is split off.The inhibitory...
- 10
- 575
- 0
Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc
... activating enzyme (E1), a conjugating enzyme (E2) and a ligase (E3) (reviewed in [14]). Of the three enzymes, E3 enzymes are the key determiningfactors in substrate protein selection. Synoviolin, ... Synoviolin is involved in the degradation of PS cofactors using syno-violin-null cells, as PS cofactors undergo the ubiqui-tin ⁄ proteasome pathway. We report that Synoviolin is involved in the ... M (2008)Novel role of presenilins in maturation and transport of integrin b1. Biochemistry 47, 3370–3378.T. Maeda et al. Synoviolin is involved in the degradation of nicastrinFEBS Journal...
- 9
- 561
- 0
Báo cáo khoa học: Functional genomics by NMR spectroscopy Phenylacetate catabolism in Escherichia coli docx
... b-ketothiolase; PaaZ, unknown, putative ring-cleavage enzyme, aldehyde dehydrogenase domain (N-terminus), MaoC-likeprotein domain (C-terminus). PaaX and PaaY may be involved in regulation. 3048 W. Ismail ... severalproteobacteria, including E. coli, Pseudomonas putida and Azoarcus evansii, contain clusters of 11–16 genes believed tobe involved in the catabolism of phenylacetate [2,5,6,9,10](Fig. 1). The paaK gene of ... the AA¢MM¢XX¢spin system using the chemical shifts and coupling constants summarized in Table 1.Fig. 7. Proposed outline of the pathway of aerobic metabolism of phenylacetate in E. coli. For...
- 8
- 310
- 0
Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx
... moderate staining in the interstitium (purple arrows). (C–E) Staining of sPLA2-V in three active RA tissues. In all cases, intense staining of the granulation tissue in the sublining interstitium ... staining of sPLA2-IIE was observed in two inactive RA sections (Fig. 4A), it was intense in VSMC in three distinct active RA sections (Fig. 4Ba–c). In contrast, staining of synovial lining ... Immunohistochemical localization of sPLA2-IID in human joints affected by RA. (A,B) Staining of two inactive RA tissues. In both sam-ples, weak and scattered staining of sPLA2-IID was seen in the lymph follicles...
- 18
- 432
- 0
Báo cáo khoa học: G protein-coupled receptor-induced Akt activity in cellular proliferation and apoptosis pptx
... (2003) Regulation of the phosphatidylinositol 3-kinase, Akt ⁄ protein kinase B, FRAP ⁄ mammalian target of rapamycin, and ribosomal S6 kinase 1 signaling path-ways by thyroid-stimulating hormone ... The luteinizing hormone-releasing hor-mone inhibits the anti-apoptotic activity of insulin-likegrowth factor-1 in pituitary aT3 cells by protein kinaseCa-mediated negative regulation of Akt. ... aconserved tertiary structure of an N-terminal pleckstrinhomology domain, a kinase domain and a C-terminalregulatory domain containing the hydrophobic motifphosphorylation site [5]. While the homology...
- 12
- 392
- 0
Báo cáo khoa học: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae An experimental and theoretical approach pot
... Vmax, Km and Kivalues of enzymes involved in the adenine and hypoxanthine metabolism in yeast cytosol. Vmaxvalues represent the average of a minimum of three determinations obtained from ... from Fig. 1 and Table 1, suggested that (a)the main if not unique source of inosine is the intracellularpool of adenine nucleotides, (b) the decrease in ATP and theincrease in inosine, promoted ... the enzymes involved in the pathway from ATP to inosine, in yeast extracts from(un)treated cells, could not explain the effect of H2O2on thelevels of ATP and inosine. Application of a mathematicalmodel...
- 12
- 506
- 0
Báo cáo khoa học: dehydrogenase from Arabidopsis thaliana, a flavoprotein involved in vitamin C biosynthesis pot
... GALDH in plants lacksthe histidine residue involved in covalent flavinylation in GUO, ALO and GLO, but contains a leucine resi-due instead (Leu56 in mature AtGALDH), indicatingthat the flavin cofactor ... cleavage site of the mitochondrial targeting sequence in plant GALDH (FR ⁄ YA). The asterisk (*) marks the histidine residue involved in covalent binding of the FAD cofactor in GUO, ALO and GLO. ... achieved after 15 min(dotted line). The dashed line and the dashed–dotted line representthe intermediate spectra observed during the reduction after 1 min and 2 min of illumination, respectively....
- 14
- 333
- 0
Báo cáo khoa học: Protein disulfide isomerase family proteins involved in soybean protein biogenesis docx
... the amounts of pro-b-conglycinin and proglycinin are considered to be nearly equivalent tothe synthesis levels of both b-conglycinin and glycinin.The synthesis of proglycinin was initiated when ... with His-Bind resin (EMDBiosciences, Inc.). After washing of the column with bindingbuffer containing 60 mm imidazole, recombinant proteinswere eluted with binding buffer containing 1 m imidazole,concentrated ... forglycoprotein folding [32,33], were determined. Pro-b-conglycinin, possessing an N-terminal prosequence, and proglycinin are transient protein forms that arepresent in the ER prior to processing in...
- 17
- 327
- 0
Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ