Báo cáo khoa học: The effect of pH on the initial rate kinetics of the dimeric biliverdin-IXa reductase from the cyanobacterium Synechocystis PCC6803 pptx
Báo cáo khoa học: Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata pot
... comparison with the one obtained at pH 6.0. No preferential loss of signal in the region of any of the aromatic residues was observed, suggestinga global loss of the tertiary structure of ovorubin.Enzymatic ... regard to pH In order to evaluate the influence of pH on ovorubin struc-ture, solutions of 0.24 mgÆmL)1 of the protein at different pH values (from 2 to 12) were prepared using sodium phos-phate ... is the role that the carotenoidpigment ASX plays in the structural stability of theseA pH BI(q *q2) Rg (Å) q (Å–1) Fig. 4. Effect of pH on native ovorubin size and shape. (A) Rg of the...
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Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf
... in the IM becoming freely permeableto protons, leading to the uncoupling of oxidativephosphorylation. MPT is caused by the opening of the nonselective, highly conductive PTP in the mitochon-drial ... from the generation of an electro-chemical gradient across the IM forms the basis of the IM wm. During cell death, MP often increases, allow-ing for the release of soluble proteins. The onlymechanism ... geneexpression and enhance the oxidative phosphorylationcapacity of liver mitochondria [30]. Studies that havefocused on HSS and the mitochondria have indicatedthat HSS not only affects the activity of...
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Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt
... interaction requires a completeMATH domain and the N-terminal region of RAP2.4 The use of a truncated version of BPM1 in the Y2Hscreens demonstrated that the BTB domain is notinvolved in the assembly ... RAP2.13.To further confirm the interaction of RAP2.4 andRAP2.13 with BPM proteins, we cloned the corre-sponding full-length cDNAs for both genes, generatedGST fusion proteins and tested these in ... lines, we saw GUS expression in the vascu-lar tissue of cotyledons and mature leaves, whereas, in the flowers, the anthers, connectives and filaments and the base and tip of the stigmata were stained....
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... obtained from injections of the metal into a solution of the protein. Typically an initial injection of 2 lL copperchelate solution was followed by a further 29 injections of 4 lL of Cu(II) into the ... oligomers are the cause of the initial insultrather than the insoluble fibrous material.Environmental factors, among them metal ions, arebelieved to contribute to the onset of Parkinson’s,Alzheimer’s ... stability of the tertiarystructure to the effect of Cu2+could be made.Influence of copper on oligomerization,aggregation and amyloid fibril formationFirst, we probed the effect of copper binding on oligomer...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... by cone voltage fragmentation [14]. The fragment ions of m/z 729 and m/z 544 are both from the monofucosylated D2b-5ion m/z 1037 and not from the difucosylated D2b-5ion m/z 1183.Thus the ... in the 6-branch, and believed to be from a contaminant (see below), having the same molecularmass with one Fuc in each of the 3- and 6-branches.Comparison of the product-ion spectra of the ... 3-linked). The location of the Fuc residue can be deduced from the CIDMS/MS fragmentation. In comparison with the spectrum of iLNO (Fig. 1A), the D2b-5ion has shifted 146 Da tom/z 1037 corresponding...
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Báo cáo khoa học: Protective effect of dietary curcumin and capsaicin on induced oxidation of low-density lipoprotein, iron-induced hepatotoxicity and carrageenan-induced inflammation in experimental rats ppt
... alterationin endothelial function, are considered among the earlyevents in the pathogenesis of atherosclerosis [43]. The alteration of oxidant ⁄ antioxidant balance may affect the susceptibility of ... influence the activity of serum alkaline phospha-tase and acid phosphatase in iron(II)-injected rats, only the combination of spice principles significantly coun-tered the elevated serum acid phosphatase ... inhibited the in vivo iron-induced LDL oxidation, aswell as copper-induced oxidation of LDL in vitro. The protective effect of the combination of curcumin and capsaicin on LDL oxidation was greaterthan...
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Báo cáo khoa học: Dual effect of echinomycin on hypoxia-inducible factor-1 activity under normoxic and hypoxic conditions docx
... enhance Sp1 activity in theseconditions. This effect was no longer observed athigher concentrations. The protein level of Sp1 waschecked in the different conditions: the results showedthat ... result of the loss of the molecule during the extraction procedure: indeed, only the nuclear proteins are recovered and the DNA is dis-carded. By contrast, when echinomycin was added to the nuclear ... in the expression of HIF-1 target genes. This effect is caused by an increase in HIF-1a pro-tein level, resulting from an increase in the transcription of the HIF-1Agene in the presence of...
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Báo cáo khoa học: Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress pdf
... scavengers used that only the generation of 1O2is responsible for the photooxidationeffects. The role of active oxygen scavengers in the protection of the structure and function of the photo-systems ... The main site of PSI photo-inhibition under aerobic conditions was then shownto be the inactivation of iron–sulfur centers [11],whereas anaerobic photoinhibition resulted in the blocking of ... degradation of PsaB gene product,one of the photosynthetic reaction center subunits of photosystem I, upon photoinhibition. Photosynth Res53, 55–63.22 Tjus SE, Moller BL & Scheller HV (1999) Photoinhibi-tion...
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Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot
... assess the net effects of redox perturbations that reflect integratedmetabolic responses, dissecting the mechanisms of theseoverall responses requires investigation of the separatecomponents of the ... pyruvatedehydrogenase phosphorylation depends on the state of the complex-bound dihydrolipoate. Thus, the redox regulation of the eukaryotic pyruvate dehydrogenase is mediated by the phosphorylation/dephosphorylation ... from the scheme of the overall 2-oxo aciddehydrogenase reaction (reactions 1–5), the steady-stateratio of the complex-bound lipoate/dihydrolipoate is a func-tion of (a) concentrations of the...
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Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf
... 23 (b) and 33 (c) of gestation (scale bar = 25 lm). The expression of CIDEC increased along with the differentiation of adipose tissue, and the highest level of expression of CIDEC was detectedin ... between the 14th and 16thweeks of gestation in humans, and that the second tri-mester of gestation is the critical period in adipogenesis.After the 23rd week of gestation, although the number of ... component of morphogenesis, maybe defined in general terms as the proliferation andsubsequent differentiation of the fat-cell lineage capa-ble of the assimilation of lipid to form a lipid-contain-ing...
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