Báo cáo khoa học: Creation of a new eye lens crystallin (Gambeta) through structure-guided mutagenic grafting of the surface of bB2 crystallin onto the hydrophobic core of cB crystallin pot
... lab works
image acquisition and analysis software was used to quan-
tify band intensities. Antibodies were purchased from Tian-
jin Saier Biotech and Sigma-Aldrich.
Statistical analysis
Data are ... LA,
Sjoblom T, Barad O, Bentwich Z, Szafranska AE, Lab-
ourier E et al. (2006) The colorectal microRNAome.
Proc Natl Acad Sci USA 103, 3687–3692.
14 Yanaihara N, Caplen N, Bowman E, Seike M, Kum...
... chromosomal
DNA of M. mazei as template and the following primers:
mm0632for, 5¢-ATGGTAGGTCTCAAATGATAGGAA
ATGAAGAAAAAATAAATAAGC-3¢; and mm0632rev,
5¢-ATGGTAGGTCTCAGCGCTGGCTTTCCAGACGCA
TTTTTTGC-3¢.
The ... A molecular mass of
20 kDa was found by SDS ⁄ PAGE, consistent with the
expected mass of 19.2 kDa of the protein monomer
(not shown). The native enzyme had a molecular...
... 3353
Creation of a new eye lens crystallin (Gambeta) through
structure-guided mutagenic grafting of the surface of bB2
crystallin onto the hydrophobic core of cB crystallin
Divya Kapoor
1
, Balvinder ... plasmid.
The gene was amplified from this plasmid by PCR using for-
ward primer 5 ¢-ACTTATACTATCCATATGGGTAAAAT
CATCTTCTTTGAACAGG-3¢ and re...
... (GE
Healthcare, Anapolis, MD, USA) to the ICU but not on the gen-
eral wards. The new system was introduced following a pro-
gram of staff training and HWP was completely changed on a
single day. The ... investigator illness. The ICU medical and nurs-
ing staff were unaware that the study was being conducted.
Ethical approval was not sought, because at the time audits
we...
... demonstrated that Asp129 of NirF
could not be essential for any function similar to that
in Asp141 of Met8P.
The idea of NirF being a dehydrogenase is appealing
because of the presence of a putative ... d
1
heme lacking iron and ⁄ or with the side chain satu-
rated, but accessing these putative substrates is not
trivial. An alternative approach would be to seek accu-
mulat...
... red).
Table 1. Average distances between CA atoms of the stefins and
catalytic residues of cysteine proteases.
Distance calculated d (A
˚
)
Papain–stefin B 23.93
Cathepsin H–stefin A 23.36 ± 0.23
Cathepsin ... that the occluding loop is rather flexible and will
adapt to structural features of the inhibitors as well as
to the packing constraints of the environment. The lar-...
... Petersburg,
FL, USA) followed by PCR with Taq DNA polymerase
(Promega, Madison, WT, USA) using the primers
5¢-CACACTACACTGGGAAGCAGAG ACTCCAGC-3¢
and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG-
3¢. The cDNA was subcloned ... 275,
10995–11001.
40 Tanaka Hall TM, Porter JA, Beachy PA & Leahy DJ
(1995) A potential catalytic site revealed by the 1.7-
Angstrom crystal structure of the amino-...
... CCATTCCAGgtgagtag 84 ctccgcagGCCGCGCCG
2 851 CTTCTCCCGgtgtgcac 403 gtccccagGCCGGATCA
364AATGTGAGgtaggaag 277 ctcctcagAAATGTGAG
4 172 CAACAAAGgtacatgc 1335 ctgtgcagGTACTGGTG
5 1028
A. Ray et al. ... variant of the SAF-1/MAZ/Pur-1
family, is expressed during inflammation
Alpana Ray
1
, Srijita Dhar
1
, Arvind Shakya
1
, Papiya Ray
1
, Yasunori Okada
2
and Bimal K. Ray
1
1 Department of Ve...
... (S1278b) the kanMX cassette from plasmid
pUG6 [12] was amplified by polymerase chain reaction
(PCR) using the primers disRAS2fwd 5¢-TAACCGT
TTTCGAATTGAAAGGAGATATACAGAAAAAA
AACAGCTGAAGCTTCGTACGC-3¢ and ... disRAS1fwd
5¢-TTCACGATTGAACAGGTAAACAAAATTTTCC
CTTTTTAGAACGACATGCAGCTGAAGCTTCGTA
CGC-3¢ and disRAS1rev CAAAACCATGTCATAT
CAAGAGAGCAGGATCATTTTCAACAAATTATGC
ATAGGCCACTAGGGATCTG-3¢. YEp351-SUT...
... for mouse Slc1 2a2 mRNA were designed as
follows; Slc1 2a2 antisense, 5¢-ATCTTCACAAGAAAAAT
CACCTGGTACCAAGGATGT; Slc1 2a2 sense, 5¢-ACAT
CCTTGGTACCAGGTGATTTTTCTTGTGAAGAT.
All experimental protocols ... 14220–14225.
18 Ohto H, Kamada S, Tago K, Tominaga S, Ozaki H,
Sato S & Kawakami K (1999) Cooperation of Six and
Eya in activation of their target genes through nuclear
translocation...