Báo cáo khoa học: The pivotal regulator GlnB of Escherichia coli is engaged in subtle and context-dependent control potx
Báo cáo khoa học: The pivotal regulator GlnB of Escherichia coli is engaged in subtle and context-dependent control potx
... implications and the mecha-nistic basis for this conditional redundancy of GlnB (and GlnK) are discussed.ResultsModulation of the GlnB concentration in vivo and the levels of GlnB and GlnK In order ... with the puzzle of a func-tional explanation for the existence of GlnB: whyshould control by PII* be absent altogether, and whatthen is the function of the cascade and of its pivot GlnB? The ... cultureswere examined. Error bars indicate the SEM. The dotted line is aresult of two linear regression fits of the data points. The extraabscissa on top of the figure indicates the molar ratio of PII*-tri-mer...
- 17
- 390
- 0
Tài liệu Báo cáo khóa học: The C-terminal domain of Escherichia coli Hfq increases the stability of the hexamer ppt
... polypeptide is a nucleic acid bindingprotein involved in the synthesis of many polypeptides.Hfq particularly affects the translation and the stability of several RNAs. In an earlier study, the use of ... transforminfra-red. On the basis of these results, we propose that the C-terminal domain could protect the interface between the subunits and stabilize the hexameric Hfq structure. The origin of this C-terminal ... microscopy and urea unfolding analysis of the truncated protein are presented in this paper. They indicatethat the C-terminal region has a structural and thermo-dynamic role in stabilizing the hexameric...
- 8
- 427
- 0
Tài liệu Báo cáo khoa học: Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS docx
... glycine, histidine, isoleucine, leucine, phenyl-alanine, proline, serine, threonine, tyrosine, and valine for[U-13C]glucose experiments and aspartate, isoleucine, leu-cine, phenylalanine, ... growth and glucoseuptake rates.While the flux profiles were similar in the three wild-typestrains with small differences in the fractions of serineoriginating from glycine and OAA originating ... major isoform of phosphofructokinase that constitutesabout 90% of the total phosphofructokinase activity [27,28].As phosphofructokinase is required for glucose catabolismvia both glycolysis and...
- 12
- 421
- 0
Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efficiency doc
... 3A,B). The presence of Ca2+increased the thermal stability of PhyH and PhyH-DII.After incubation at 60 °C for 30 min, both enzymesretained > 70% of their initial activity in the presence of ... Ala208 and Gly234). Some of the res-idues involved in phosphate and calcium binding areconserved in the PhyH-DII sequence, whereas onlyone phosphate-binding residue is conserved in the PhyH-DI ... assessed.ResultsMicroorganism isolationUsing phytase screening and low phosphate media,three strains with phytase activity were isolated from the alpine tundra soil of China No. 1 Glacier in Xinji-ang, China. Strain...
- 9
- 801
- 0
Tài liệu Báo cáo khoa học: The thioredoxin-independent isoform of chloroplastic glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation docx
... glutaredoxin, might be required for reduction of the mixed disulfide. In this hypothesis, glutaredoxinswould be involved in a signaling pathway contributingto the redox regulation of the Calvin cycle ... down-regulation of the Calvin cycle understress would allow redistribution of the reducing power in the chloroplast by decreasing NADPH consumption and thereby increasing electron availability at the redu-cing ... Aliquots of the incubation mixtures werewithdrawn at the indicated time points and the remaining NADPH-dependent activity was determined. Activity is given as a percent-age of the initial activity.Glutathionylation...
- 15
- 515
- 0
Tài liệu Báo cáo khoa học: "The grapho-phonological system of written French: Statistical analysis and empirical validation" pdf
... characteristics of this mapping. The results indicate that human naming performance is influenced by the frequency of graphemic units in the language and by the predictability of their mapping ... resolution of these issues. In this paper, we present a descriptive analysis of the grapheme-phoneme mapping system of the French orthography, and we further explore the sensitivity of adult ... the 436 statistical structure of the language? We believe that, in the field of visual word processing, the lack of precise quantitative descriptions of the mapping system is one factor...
- 7
- 502
- 0
Tài liệu Báo cáo khoa học: The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Purification and properties docx
... determine the N-terminal amino acid sequence of the larger subunitusing a number of different preparations of the protein. The gene encoding the smaller subunit (fdh1B)wasidentified in the unfinished ... shown in blue. Amino acids coordinating the two molybdopterin guanine dinucletoide cofactors of FdhH of E. coli (MGD801 and MGD802)thatwere found to be invariant among molybdopterin-containing ... 4),as well as its brownish/yellowish colour were indicative of the presence of cofactors, and this finding is in agreementwith the primary sequence analysis data (see above). The spectrum shows shoulders...
- 9
- 461
- 0
Báo cáo khoa học: The N-terminal region of the bacterial DNA polymerase PolC features a pair of domains, both distantly related to domain V of the DNA polymerase III s subunit ppt
... the PolC N-terminus is involved in mediating the PolC-s interaction and possi-bly also in binding DNA.IntroductionGenome replication in bacteria is carried out by the multicomponent protein ... and PolC at the domain level. These include the proofread-ing 3¢–5¢ exonuclease domain, oligonucleotide ⁄ oligo-saccharide-binding (OB) domain and the additionalN- and C-terminal regions in ... PolC and DnaE, respec-tively. The PolC proofreading 3¢–5¢ exonucleasedomain is inserted into the PHP domain and is anintegral part of the polypeptide chain, whereas DnaEuses a separate proofreading...
- 10
- 419
- 0
Báo cáo khoa học: The oleic acid complexes of proteolytic fragments of a-lactalbumin display apoptotic activity pdf
... in many protein folding studies[2,3]. The 123 residue chain of a-LA is organized intoa discontinuous a-helical domain composed of residues 1–39 and 81–123, and a small b-domaincomprising the ... complex. This view is in line with the fact that all variants of a-LA of human, bovine,equine, porcine and caprine origin, as well as recombi-nant mutants of a-LA devoid of Ca2+-binding proper-ties, ... variation in amino acid sequence of the polypeptide chain of a-LA, severe truncation of the polypeptide chain of the protein is also tolerated in the formation of biologically active OA complexes.Therefore,...
- 11
- 399
- 0
Báo cáo khoa học: The Rieske protein from Paracoccus denitrificans is inserted into the cytoplasmic membrane by the twin-arginine translocase doc
... chains.Another interesting outlook is the identification of aputative cytosolic binding partner of the ISP, e.g.by using chemical cross-linking approaches and MS,giving interesting insights into ... effects. The [2Fe)2S] cluster of the Rieske protein is inserted in the cytoplasmAs cytosolic cofactor insertion is one of the hallmarks of Tat translocation, the cofactor loading status of the ISP in ... fractions of the C152S mutant, demonstrating: (a) the inability of the mutant to insert a cofactor, and (b) the specificorigin of the signal in the other samples. The cytosolicfraction of the complemented...
- 14
- 535
- 0
Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ