Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot

... intermediate exchange regime. Thus, the conﬁguration at the hemiacetal carbon of galactosemay affect the dissociation constants.By contrast, because chemical-shift changes upon the addition of sugars ... the center of the broadened signalsshifted to the position of the second signal correspond-ing to the bound state during titration ([sugar] ⁄[EW29Ch] = 0.5–3) and the resonance signal of the side-chain ... of NH2resonances of aspara-gines and glutamines are connected byhorizontal lines. The side chains of NHresonances of tryptophan are marked by‘sc’. Interaction of EW29Ch with sugars H. Hemmi...

Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

... volumes of 5M urea solution and then run on SDS ⁄ PAGE. Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the ... corresponding interaction in rabbitTnI–TnC in the absence of divalent cation. Therefore,this interaction potentially participates in both the Ca2+-dependent activation of the contraction and the maintenance ... site, from actin, resulting in the release of the inhibitionand muscle contraction [17]. The structural TnC-bind-ing site interacts with the C -domain of TnC in both the relaxed and contractile...

Báo cáo khoa học: Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily doc

... a stepwise manner, using an i ncreasingconcentration of acetonitrile in the elution solution, andloaded directly on the MALDI target by using the drieddroplet technique and a-cyano-4-hydroxycinnamic ... emonstrate the nature of the oxidized enzyme and the extremesensitivity of C175 and C547 to changes in redox conditions.Different data were generated from the MALDI-TOF-MS analysis of a cN-II ... mMMgSO4and 0.7 U of Platinum Pfx DNApolymerase in PCR reaction buffer. The cycling conditionsin the second PCR were the same as those used in the ﬁrstPCR, but in the second PCR, the annealing...

Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx

... The time course of the production of transfer productsand the degradation of 1 was observed by HPLC analysis asshown in Fig. 1B. The maximum production of 9 and 10was reached at 4 h, and their ... yields (82 and71%) depending on the acceptor. The positional isomers 5and 6 were prepared simultaneously by Gal transfer from Galb-oNP to the OH-3¢ and OH-6¢ positions of 3 usingB. circulans b-D-galactosidase-mediated ... under the experimental conditions,Fig. 1. HPLC analysis of the reaction mixture obtained by endo-b-galactosidase-mediated transglycosylation and time courses of the pro-duction of transglycosylation...

Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt

... fhydrogen bonds of the side c hain of Arg78 for the simulations in Me2SO; the agonist forms a significantlyhigher number of interactions than the antagonist. For the simulations in water, the differences ... role in the Fig. 4. Backbone cross RMSD matrix for the comparison of the various conformers of the five MD simulations of Table 1. The x and y axescorrespond to the simulation time of the various ... flexible, with Ala81 far distant from the side chain of Arg78. The side chain of Arg78 is lesswell defined, as in the case of aqueous solution, because of the absence of interactions with the s ide...

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... allowed regions of the Rama-chandran plot. An additional 6% of residues werefound in the generously allowed region. None of the res-idues of helices 2 and 3 was found in the disallowedregions. Instead, ... additionalupper distance constraints. The 3D structure of micelle-associated VpUcyt was cal-culated on the basis of the ﬁnal set of proton–proton dis-tance constraints using cyana, a software ... important aspect is the topology of the cytoplasmic domain of VpU on a membrane. Solidstate NMR suggests an orientation of helix 2 parallelto the membrane surface; data on helix 3 are againcontradictory...

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... show any activitymust therefore be a consequence of the reactivity of the functional group and further support the hypothesisthat E52 acts as an initiating base in the reaction.R105 mutationsR105HR105 ... stabilize the transition state andhold E52 in the correct position to initiate the catalyticreaction. The transition state of this type of kinases isconsidered to be close to trigonal bipyramidal ... for the nucleoside analogues.This must be a consequence of the tryptophan creatingsteric hindrance for the purines. The intention behind the mutation of Y70 to W was to create an enzyme with increased...

Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx

... turn)folded structure. The conformational features of AII in the solution state (representing the free ligand conformation)were compared with the conformation of the hormonecomplexed to the high-afﬁnity ... in conferring the structure of AII.X-ray structure of AII bound to the mAb Fab131:comparison with the NMR solution structure The phenomenon of conformational stabilization [55] andselection ... 2003 On the molecular basis of the recognition of angiotensin II (AII) NMR structure of AII in solution compared with the X-ray structure of AII boundto the mAb Fab131Andreas G. Tzakos1, Alexandre...

Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf

... a-helices on one side of the b -sheet and two on the other side. The rmsd of the heavy atoms (Ca, C, andN) of the protein core, when the NMR structures of both conformers are superimposed on the crystal ... recognition are in accord with the hypothesisthat the C -domain could be involved in the regulation of translation termination. As Asp359 and Gly363 areimportant for stabilization of both conformations ... ribbon representation of the backbone of the C-terminal domain of human eRF1.Fig. S6. The order parameter, S2, calculated separatelyfor the open and closed conformers of the C-terminal domain...

Báo cáo khoa học: NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease ppt

... presented the dissociation constant of 3measured by NMR and, as additional proof for bind-ing of 3 to the substrate-binding site, demonstratedchanges in cross-peak positions for residues lining the substrate-binding ... in the crystal structure with BPTI [4] and wasconﬁrmed by narrow NMR line shapes. The resonances of the complex with 1 were assignedusing NS2B–NS3pro, whereas the 3D NMR experi-ments of the ... Intermolecular NOEs with the aromatic ring protons of 3 are marked with their assignments. Several of the NOEs arealso observed with the methyl groups of 3at 2.3 p.p.m. NMR analysis of the West Nile...

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