Báo cáo khoa học: Vitamin D stimulates apoptosis in gastric cancer cells in synergy with trichostatin A ⁄sodium butyrate-induced and 5-aza-2¢-deoxycytidine-induced PTEN upregulation ppt

... NaBu and 5-Aza.Results Vitamin D induced apoptosis in gastric cancer cells through PTEN upregulation Earlier studies revealed that vitamin D was able toinduce cell differentiation and cell cycle arrest, ... 4. Vitamin D enhanced the apoptosis induced by TSA ⁄ NaBu. HGC-27 gastric cancer cells were treated with VD3 and 200 nM TSA (A) ,or VD3 and 2 mM NaBu (B), and western blotting was used to evaluate ... investigated the effect of PTEN on the apop-tosis induced by DNA methylation and vitamin D in gastric cancer cells. We treated HGC-27 cells with 5-Aza and VD3, either alone or in combination, and we...

Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

... novel variants of human medium chain acyl-CoAdehydrogenase (MCAD)K364R, a folding mutation, and R256T, a catalytic-site mutationresulting in a well-folded but totally inactive proteinLinda P. ... released to be further degraded via the b-oxida-tion cycle [1].Defects in MCAD accordingly impair the ability todegrade fatty acids, and MCAD deﬁciency is the mostfrequently diagnosed clinical ... studied in rat MCAD, where the arginine was mutated toalanine, lysine, glutamine and glutamic acid. Theauthors found that the lysine mutant exhibited signiﬁ-cantly reduced activity, whereas...

Báo cáo khoa học: The organotellurium compound ammonium trichloro(dioxoethylene-o,o¢)tellurate reacts with homocysteine to form homocystine and decreases homocysteine levels in hyperhomocysteinemic mice pptx

... there are several homocysteine-loweringagents available. Cobalamin and vitamin B6are admin-istered to patients with hyperhomocysteinemia causedby a lack of these factors, and vitamin B6is also ... pur-chased from Gibco Laboratories (Grand Island, NY, USA).Caspase-3 and PARP1 antibodies were purchased from CellSignaling (Danvers, MA, USA). Antibody against a- tubulinwas purchased from Sigma. AS101 ... homocysteine levels are a marker for severalpathologies, notably cardiovascular and neurodegener-ative disorders, and several homocysteine-reducingagents, such as vitamin B6, vitamin B12, and...

Báo cáo khoa học: Stage-speciﬁc expression of Caenorhabditis elegans ribonuclease H1 enzymes with different substrate speciﬁcities and bivalent cation requirements ppt

... RNA–DNA hybrids in vitro [17] and has been defined as a dsRNA and RNA–DNAhybrid-binding domain (dsRHbd) [18]. The functionof the dsRHbd in eukaryotic RNase H1 has also beendiscussed. The dsRNA ... con-taining 6 m urea, loaded on to a HiTrap Desalting Col-umn, and desalted with buffer C. Purified recombinantproteins were analyzed by SDS ⁄ PAGE on a 10–20% gradi-ent gel and stained with ... using H0AB-S(5¢-CCAGTTACTCAAGATTTTGAACGC-3¢) as a for-ward primer and H0AB -A (5¢-CGTTTAATGAACATTTGGGCTCC-3¢) as a reverse primer. PCR products werepurified with GFX PCR DNA and a Gel Band...

Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc

... 5C ,D) . In addition,Ac-DEVD-CHO, a caspase-3 inhibitor, inhibited trog-litazone-induced adipocyte apoptosis and caspase-3cleavage (Fig. 5E–G).The Bcl-2-dependent and caspase-3-dependent apop-tosis ... troglitazone-induced adipocyte apoptosis was, indeed, inhibited in the presence of GW9662. In addition, pioglitazone, another TZD, and PGJ2, whichdirectly binds to and activates PPARc [29], also induceadipocyte ... evidence from several in vivo and in vitro studies has indicated that apoptosis is a signiﬁcant factor in adipocyte depletion duringweight reduction [5–8]. In addition, an animal model ofadipocyte...

Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... treated with EGF and ADR asdescribed in (A) , harvested at the indicated times after the additionof ADR, and used for immunoblot analysis of pro-caspase 9 and caspase 9. (C) Cells were treated ... treated with EGF and ADR as described in (A) . Lysates were prepared at the indicated times after the ADRaddition and analyzed for caspase 9 activity by using a ﬂuorometricsubstrate-based assay. Each ... chemo- and irradiation therapy-induced cell death [36]. The A BC D Fig. 6. Protective action of EGF againstADR-induced apoptosis is AP1 dependent. (A) TAM1 and TAM2 cells were cotransfect-ed with...

Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

... the incidentwavelength. Diffraction spots were recorded on a MAR345 image plate using the oscillation method [36].Data indexing and scaling were carried out with DENZO and SCALEPACKsoftware, ... codon GCA, and the Thr225 codon ACT was changed into the Alacodon GCT. The mutagenized GAPDH gene fragmentswere then excised from the plasmid by digestion with SalI and SacI and used to replace ... sickness,Chagas disease, leishmaniases) and domestic animals in tropical and subtropical regions. Today, the medical and economic problems caused by the trypanosomiases repre-sent a formidable obstacle...

Báo cáo khoa học: Programmed cell death Apoptosis and alternative deathstyles pot

... rlinden@biof.ufrj.brAbbreviations: AIF, apoptosis inducing factor; DAPk, death-associ-ated protein kinase; DRP, DAPk-related protein kinase; FADD,Fas-associated protein with death domain; IAP, inhibitor of apoptosis; LEI, ... deﬁcient in caspase-8, the forced multimerization of Fas-associatedprotein with death domain (FADD) induced caspase-independent cell death. No DNA fragmentation wasobserved and dying cells showed ... TNFa-induced apop-tosis, and amino acid and protein deprivation enhancedTNFa-induced autophagy, but not apoptosis. These datasuggest that, at least in this case, early stages of autophagy are...

Báo cáo khoa học: 7-Ketocholesterol-induced apoptosis Involvement of several pro-apoptotic but also anti-apoptotic calcium-dependent transduction pathways ppt

... cellsurvival, the Ras ⁄ Raf ⁄ MEK ⁄ Erk pathway plays a crit-ical role. Indeed, Ras-activated Raf operates by phos-phorylating and activating MAPK-Erk kinase-1 and -2 (MEK 1 ⁄ 2) [9,10]. MEKs have ... quantiﬁcation of cells with fragmented and ⁄ or condensed nuclei was performed usingHoechst 33342 and the percentage of apoptotic cells was deter-mined. Data are means ± SD (n > 5).Pathways in 7-ketocholesterol-induced ... could be involved in MAPK activa-tion and as we and others have previously described a calcium in ux in oxysterol-induced apoptosis [8,21,22],we tested whether or not calcium could mediateERK...

Báo cáo khoa học: Cholecystokinin rapidly stimulates CrkII function in vivo in rat pancreatic acini Formation of CrkII–protein complexes docx

... anincrease in the upper band detected within 1 min afteraddition of CCK-8 and a maximum reached within 5 minFig. 1. Identiﬁcation of CrkII in rat pancreatic acini (A, B) and CCK-dependent induction ... Jensen3 and Luis J. Garcia-Marin11Departamento de Fisiologia, Universidad de Extremadura, Caceres, Spain;2Departamento de Bioquimica, Biologia Molecular yGenetica, Universidad de Extremadura, ... [33]. In addition, v-Src and v-Crktransformed cells display elevated tyrosine phosphorylationon proteins related with CrkII and focal adhesions, inclu-ding p130Cas,p125FAK and paxillin [10,33].The...

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