... 9,11 5–1 24.24 Sharma Y, Rao CM, Narasu ML, Rao SC, Somasunda-ram T, Gopalakrishna A & Balasubramanian D (1989)Calcium ion binding to d- and to b-crystallins. Presence of EF-hand motif in d-crystallin ... calori-metry, analytical gel filtration and dynamic lightscattering of the apo and holo forms of bA3- andbB2-crystallins. There was no significant change in the stability and hydrodynamic radius of the ... affinity of the cation for the calcium-binding site than the dye. Calcium displacedStains-All to a lesser extent from bA3-crystallin than from bB2-crystallin, indicating lower affinity of calcium for...