... termed the a-crystallindomain, distinguishes a ll small heat shock/a-crystallinproteins, and a two or three domain structure is proposed for these proteins [7,8]. The a-crystallin domain, locatedtoward ... °Cand37°C, althoughtesting at higher temperatures may be informative.Additionally, the C-terminal extension of p26 is required for full induction of thermotolerance in E. coli and thusmay ... blue/whitescreening using the LacZ system, propagated in LB broth, and examined by restriction analysis for plasmids incorpo-rating inserts of t he appropriate size, which were subclonedinto the prokaryotic...