Báo cáo khoa học: Action of palytoxin on apical H+/K+-ATPase in rat colon pdf

... yetbeen demonstrated. Thus, in the current investigation, wedescribe the action of palytoxin on the H+/K+-ATPasefrom the rat colon and demonstrate that the interaction of this toxin with the ... latter findings, as the palytoxin- inducedconductance was insensitive to 1 mMouabain while beinginhibited at low concentrations of orthovanadate.Similar findings showing a palytoxin- induced K+effluxthat ... 3907Ionic selectivity of the palytoxin- induced poreAssuming that palytoxin might induce cation-permeablepores in the apical membrane, the Isc evoked by the toxinshould consist of an in ux of...

Báo cáo khoa học: Transport of L-arginine and nitric oxide formation in human platelets pdf

... volumes of cold NaCl/Picontaining 10 mML-arginine. The radioacti-vity corresponding to the incorporatedL-[2,3,4-3H]argininewas directly measured by liquid scintillation counting of the ... Moreover in endothelial cells [36] extracellularL-arginine concentrationis the most determinant of L-arginine availability for cNOS,despite the fact that intracellular arginine concentrationsgreatly ... rate of L-arginine transport under physiologi-cal conditions it would be suitable to measure the transport in the presence of plasma concentrations of competingamino acids. In addition both...

Báo cáo khoa học: "Analysis of Mixed Natural and Symbolic Language Input in Mathematical Dialogs" pdf

... Patient of type FORMULA is a subformula embedded within aFORMULA in the Actor relation with respect to the head contain’]contain( , )CONTAINMENT(container , containee )[’the Containment relation ... aforementionedpublications.4.3 Domain interpretationThe linguistic meaning representations obtainedfrom the parser are interpreted with respect to thedomain. We are constructing a domain ontologythat ... meaning of the action verbs” is needed for theinterpretation of the intended proof-step.Metonymy Metonymic expressions are used torefer to structural sub-parts of formulae, resulting in predicate...

Tài liệu Báo cáo khoa học: Effects of sequestration on signal transduction cascades docx

... Solid line: the case of kinase sequestration,dotted line: no kinase sequestration. Grey lines indicate the stimu-lus (i.e. kinase concentration), scaled by 0.1 in (A).Effects of sequestration ... concentrationand total kinase concentration, respectively, and KMisthe Michaelis–Menten constant of the kinase. The con-centration of the complex [TK] approaches the totalconcentration of ... response of the active fraction to a change of thekinase concentration as a function of the concentra-tions of the two forms ([T] and [T*]) and the elastici-ties of the enzymes by the following...

Tài liệu Báo cáo khoa học: Effect of gadolinium on the ryanodine receptor/ sarcoplasmic reticulum calcium release channel of skeletal muscle docx

... thevisualization of the protein composition of the fractions.Ryanodine-bindingRyanodine-binding assays were carried out using [3H]ry-anodine in a medium having the composition of 1 m NaCl,S. ... aprotinin, 1 lm leupeptin, 1 lm pepstatinA and 1 mm benzamidine. Protein concentration of HSRvesicles was determined according to the method of Lowryet al. [21], using bovine serum albumin as ... effect of changing cis calcium con-centration on the inhibitory effect of cis gadoliniumwas also investigated. When calcium concentration wasincreased from 100 lm to 1 mm in the presence of 4.5...

Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

... complete inhibition of TNFa-primed/fMLP-stimulated MPO release under conditions in which OÀ2generation was only marginally suppressed(Fig 4). Butan-1-ol alone, up to a concentration of 3%, ... [3H]PtdButaccumulation over untreated controls. Inserts show the concentration effects of brefeldin A or wortmannin on OÀ2generation and PLD activity.Data represent a single experiment representative of three ... identi-fied as the predominant isoform with only marginal expression of PLD2 in two independent experiments. Equal amplification of b-actintranscripts confirmed identical total RNA content of the samples.(B)...

Báo cáo khoa học: Effects of thymoquinone on isolated and cellular proteasomes pot

... mutation consists of a single G ﬁ A transi-tion in codon 237, resulting in a missense mutation of methionine to isoleucine [32,33]. Interestingly, a studyconducted by Van Meir et al. on different ... that TQ preferen-tially binds to the active site at low concentrations,resulting in the observed inhibition, whereas at higherconcentrations the binding to the additional sitebecomes signiﬁcant, ... existence of additional TQ-binding sites on the proteasome.Figure 4 indicates that the formation of b-subunit–TQadducts increases at TQ concentrations of 5 and20 lm, whereas it decreases at a concentration...

Báo cáo khoa học: Effects of salt on the kinetics and thermodynamic stability of endonuclease I from Vibrio salmonicida and Vibrio cholerae potx

... stoichiome-tries of participation of water, cations and anions in speciﬁc and non-speciﬁc binding of lac repressor toDNA. Possible thermodynamic origins of the ‘‘gluta-mate effect’’ on protein–DNA interactions. ... concen-trations or low temperatures, the gain in entropy on release of ions is reduced and substrate binding istherefore weaker [33]. This makes binding of highlycharged DNA very challenging ... substrate bindingand dissociation rate constants, as found in the coldadaptation of cod trypsin [39], or, in the case of VsEndA, may be linked in some way to cold adapta-tion, where an increase...

Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modiﬁcations and in enhanced protective activity in sepsis doc

... detectablebinding to CRP at the concentration and during theperiod of observation used in the experiment.Interaction analyses using increasing concentrations of the native and of the low-pH ... antigen–antibody interaction in the presence of increasing concentrations of NaCl. In both experiments, binding intensities are represented in RU,and each data point shows the mean value ± standard deviation ... dissociation phases of the interaction were monitoredfor 5 min. The regeneration of the chip surface wasperformed using a 5 m solution of guanidine-HCl (Sigma-Aldrich). The binding to the surface of...

Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx

... 57, 858–865.36 Nakagawa Y, Minakuchi C, Takahashi K & Ueno T(2002) Inhibition of [3H] ponasterone A binding byecdysone agonists in the intact Kc cell line. Insect Bio-chem Mol Biol 32, ... acid alignment of linker region andligand-binding domain (LBD) of ecdysone receptors.Fig. S2. Amino acid alignment of linker region andligand-binding domain (LBD) of ultraspiracle ⁄ RXRreceptors.This ... 100Fold inductionConcentration (µM)0.1 1 10 100Concentration (µM)0.1 1 10 100Concentration (µM)LdEcRB/VP16-LdUSP01020304050Fold inductionLdEcRA/VP16-LdUSP01020304050Fold inductionDmEcRB2/VP16-DmUSPABCmurAponAmakAFig....

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