... further
efforts were made to track down the actual target of the
inhibitors.
DISCUSSION
The purpose of t his work was to identify the key residues
in the C-domain a nd their potential role in the ... identify their target in the C-domain using peptide
mapping. T hus the inhibitory effect may be due to
interference with other reactive residues in NRPS modules...
... Three of them (CRB-3–5) are in the
vicinity of two putative coiled-coil domains, which
suggests that they may participate together with the
coiled-coil domains in folding of the N-terminal half
of ... feature of Mrs2p is the existence
of two transmembrane-domains and a short connect-
ing sequence of about 7–8 amino acids. This is sup-
posed to be the only part of...
... domain swapping mechanism involving the
core and COOH-terminal domains of the two subunits.
We hypothesized that the shortened linker in the
mutant enzyme destabilized the interaction between
the ... important, then it
is likely that the binding involves an interaction with
two regions of DNA at the point of crossing. One
hypothesis to explain how the enzyme provides...
... linkage of the active
site Ser of the protease to the carboxyl group of P
1
by an
ester bond and insertion of the N-terminal part of the RCL
as strand 4 in b-sheet A (s4A) of the serpin. Consequently,
the ... behaviour of the serpin.
Complex formation between serpins and their cognate
proteases is fuelled by the thermodynamic properties of the
serpin. Acc...
... intermediate of NO synthesis [26]. On the other
hand, in contrast with Asn130 in arginase I, the corres-
ponding Asn149 of arginase II was not found to be a
ligand for the a-carboxylate group of the transition
state ... ligand for the a-carboxyl
group of arginine in arginase I [9,23,24]. On this basis,
direct repulsion of the negatively charged Asp130
would expla...
... FEBS 4773
of Q
A
–
in PSII centers that are affected by the nonsatu-
rating concentration of inhibitor [7,8]. The E
m
of Q
A
is
raised in the presence of DCMU in the Q
B
pocket,
making it energetically ... to the reduction of the PQ pool.
Results
As reported in the literature, the I step of the O–J–I–P
fluorescence transient cannot be clearly distinguished...
... C-terminal region, including the PAS-B
domain of BMAL1, is required for the binding to DEC1,
Fig. 4. Identification of the domain of D EC1
that interacts with BMAL 1. Interactions of
various DEC1 ... region of DEC1, including the
bHLH domain, interacted with the C-terminal r egion o f
BMAL1 in a mammalian two-hybrid assay. Accordingly, a
Fig. 5. Analysis of DEC1 mutan...
... structure of the N-terminal
domain of the human homologue, solved at 2.2 A
˚
resolution [11]. The obtained model covers residues
254–568 of HpPex5p, corresponding to the C-terminal
half of the protein ... coordinates are available from the
authors upon request.
Addition of the L2 peptide was possible due to the
conserved binding site and the presence of a YQSKL...
... flavin-binding domain of ferre-
doxin : NADP
+
reductase [10]. The lysine residue is
not conserved; instead, an arginine residue interacts
with the FAD molecule of the enzyme. In the case of
FMN-binding ... Blanche F (1995) Purification of the two-enzyme sys-
tem catalyzing the oxidation of the d-proline residue of
pristinamycin IIB during the last step of pristi...
... (two
cDNAs). Initial mapping of the region of PP2Ac
involved in TIPRL binding was obtained from the
cDNAs that showed positive interaction with TIPRL.
The extension of these cDNAs is shown in Fig. ... with
GST–a4 in the presence of His–PP2Aca (data not
shown). Similar results were obtained using the
PP2Ac-binding domain of a4, a4D222 [24], instead of
the full-len...