Báo cáo khoa học: Functional expression of Pseudomonas aeruginosa GDP-4-keto6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose docx
Báo cáo khoa học: Functional expression of Pseudomonas aeruginosa GDP-4-keto6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose docx
... Functional expression of Pseudomonas aeruginosa GDP-4-keto-6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose Minna MaÈki1, Nina JaÈ rvinen1, ... GDP-4-keto-6-deoxy-D-mannose reductase (RMD). Here, we describe the enzymatic c har-acterization of P. aeruginosa RMD expressed in Sacchar-omyces cerevisiae. Previous success in functional expression of bacterial ... time -of- ¯ight MS, and ®nally NMR spectroscopy.The functional expression of P. aeruginosa RMD inS. cerevisiae will provide a tool for generating GDP-rham-nose f or in vitro rhamnosylation of...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... characterization of quinoline-degrading bacteria. Syst. Appl. Microbiol. 10, 185–190.Ó FEBS 2003 Expression of quinoline 2-oxidoreductase genes (Eur. J. Biochem. 270) 1577 Functional expression of the ... availability of a system for the functional expression of genes coding for molybdenum hydroxylases is a prerequisitefor the construction of enzyme variants by mutagenesis. Forthe expression cloning of ... those of wild-type Qor. Release of CMP upon acidic hydrolysis of theQor proteins suggested the presence of the MCD form of thepyranopterin cofactor; the CMP contents of the threeenzymes were similar.Keywords:...
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
... the control of the GAL10 pro-moter. The induction of the yeast, by galactose containingmedia, results in overexpression of GAL4, in turn inducingan increase of the expression of the OR gene ... Identifica-tion of ligands for olfactory receptors by functional expression of a receptor library. Cell 95, 917–926.20 Hatt H, Lang K & Gisselmann G (2001) Functional expression and characterization of ... 2004)doi:10.1111/j.1742-4658.2004.04494.xThe functional expression of olfactory receptors (ORs) is a primary require-ment to examine the molecular mechanisms of odorant perception and cod-ing. Functional expression of the rat...
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Báo cáo khoa học: Functional characterization of ecdysone receptor gene switches in mammalian cells docx
... 1.647 nm (confidenceinterval of )0.5963 to 3.891, R2 of 0.9292, degree of freedom of 10) and 1.447 nm (confidence interval of 0.9588–1.935, R2 of 0.9788, degree of freedom of 28),respectively. These ... versions of GEV format switches were made by replacinghelix 12 of EcR with VP16 activation domain. Thisversion of GEV supported constitutive expression of reporter gene expression in the absence of ... the control of four tan-dem repeats of EcREs inserted upstream of a thymi-dine kinase gene minimal promoter. This switch wasreported to have high level of ligand induced transgeneexpression...
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Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx
... 1469activity which was apparently produced by the E. coli expression strain. Expression pattern of mce-1::gfp fusionconstructs in C. elegansTo determine the expression pattern of the MCE-1, ... weregrown to an optical density of 0.6–0.8 at 600 nm. Then, expression of the epimerase was induced by the addition of isopropyl thio-b-d-galactoside to a final concentration of 0.5 mm. Cultures were ... are theresult of partial exclusion of the fluorescence from thecontractile elements of the muscle. Similar expression is seen in all detectable larval stages (Fig. 8D,F).Feeding of double-stranded...
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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt
... temperatures leads to differential expression of alternative forms of the LDH-A gene in white skeletal muscle of weatherfish, Misgurnus fossilis.Two isoforms of LDH-A mRNA were isolated and characterized: ... quantitative strategy to offset the lack orexcess of kinetic energy (as a measure of temperature)and its effect on enzyme activity.Alternatively, we know of a number of examples of qualitative strategies, ... tetramer. In warm-acclimated fishthe relative expression between a and b isoforms of the LDH-A mRNA isaround 5 : 1, whereas in cold-acclimated fish expression of mRNAbldhÀaisreduced almost to zero....
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Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu pptx
... expression of CitrusFLSThe unequivocal assignment of genes requires the functional characterization of the corresponding polypeptides, which has been occasionally neglected in the process of ... molecular evolution of diversity concerning the enzymes of flavonoid biosynthesis.We report the expression of highly active Citrus unshiuFLS in Escherichia coli and the purification of the labileenzyme ... bygenes of moderate to high sequence identity (from 19% to75%), which might catalyse very diverse reactions irrespect-ive of their relative degree of sequence conservation.Nevertheless, many of...
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Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot
... presence of provided structural CHH implies the level of the complexity of physiologi-cal regulation in crustaceans. Much less is known of the functions of non-ES CHH than of those of its counterpart ... on the surface of the PO andmany nerve fiber tracts and varicosities in trunks of the PO.Cloning and sequencing of PO-CHH cDNAThe first PCR of PO cDNA with a combination of a set of primers LF1 ... sensitive to homeostasis of hemolymph.Cloning of cDNA of PO-CHH of Cal. sapidus pro-duced only one size (2004 bp) that is translated intoPO-CHH neuropeptide. The cDNA sequence of PO-CHH encodes a...
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Báo cáo khoa học: Homologous expression of a bacterial phytochrome The cyanobacterium Fremyella diplosiphon incorporates biliverdin as a genuine, functional chromophore doc
... activity of the Prform of CphBcy was clearlystronger than the activity of the Pfrform, which reached, maximally, 20% of that of the Prform. Tak-ing into account the maximal conversion of Printo ... indicat-ive of the presence of a peptide with a bound chromo-phore (Fig. 3). None of these peaks matched theretention time of a free chromophore control sample.Subsequently, one of these peaks ... any loss of absorption. A comparison of Fig. 2. Absorption and absorption difference spectra (Pr) Pfr )of CphBcy from homologous expression in F. diplosiphon SF33. Inset:Comparison of CphBcy...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... (1998) Clon-ing and sequencing of the nrdF gene of Corynebacteriumammoniagenes ATCC 6872 encoding the functional metallo-cofactor of the manganese-ribonucleotide reductase (Mn-RRase). Biotechnol ... we report data on homologous expression of the nrdF gene of C. ammoniagenes strainATCC 6872. This is the first report of the successfulpurification of high amounts of the native C. ammoni-agenes ... the bulk of larger iron pro-teins. In summary, our protocol led to the enrichment of highly active R2F, in which at least 50% of the ori-ginal radical concentration of the metallocofactor wasretained...
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