Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx
Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx
... modules. The binding site for protein S mainly involves the first of the three b-chain CCPs (CCP1). However, recently it has beensuggested that CCP2 of the b-chain also contributes to the binding of ... CCP2 had no effect on binding of protein S The b-chain constructs were tested in three different assaysfor their ability to bind protein S. In the radioligand blottingassay, proteins were separated ... First, the effect of increasing amounts of fluid phase protein S on the binding of the MoAbs toimmobilized recombinant b-chain was tested. The binding of both MoAb 15 and MoAb 44 to the b-chain wasFig....
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Báo cáo khoa học: A functional polymorphism at the transcriptional initiation site in b2-glycoprotein I (apolipoprotein H) associated with reduced gene expression and lower plasma levels of b2-glycoprotein I docx
... liver lysates was measuredusing the Bio-Rad Protein Assay Kit (Bio-Rad Laboratories,Hercules, CA, USA) according to the manufacturer s instructions. Bovine serum albumin was used as standardto ... polymorphisms.This confirms our hypothesis that the effect of the Trp316Ser polymorphism was due to its non-randomassociation with the )1CfiA mutation and that the effect of the Cys306Gly is independent, ... downregulates b2GPI gene expression.Effect of the )1CfiA mutation on binding of trans-acting factorsAs the )1CfiA mutation disrupts the consensus sequencefor the b2GPI transcriptional initiation site...
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Tài liệu Báo cáo khoa học: Activated Rac1, but not the tumorigenic variant Rac1b, is ubiquitinated on Lys 147 through a JNK-regulated process docx
... mechanisms and homologous E3 ubiquitinligases are involved in both cases.On the basis of structural studies, Lys147 is asolvent-accessible residue located far from the GTP -binding site, is not involved ... Each of the 18 lysine mutants of the myc–Rac1L61sequence and the ubiquitin mutants UbR48 and UbR63were obtained by replacing the lysine codons AAA and AAG by the arginine codons AGA and AGG respectively.Rac1L61G189 ... antibodies. Expression of transfected and endogenous proteins was monitored in total protein extracts by immunoblotting using the indicated antibodies. (B) In ubiquitinated Rac1L61, ubiquitin moieties...
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Báo cáo khoa học: Signal peptide hydrophobicity is critical for early stages in protein export by Bacillus subtilis ppt
... exacerbated by the fact that the H-domains of signal peptides lack lysineresidues, which are required for cross-linking with the lysine-specific reagent DSS. Nevertheless, binding of B. subtilis Ffh ... localization of proteins in B. subtilis wasdetermined by protoplasting and subsequent trypsin accessi-bility assays. Protoplasts were prepared from B. subtiliscells in the late exponential growth phase, ... blotting using the proteinsfrom total cell extracts separated by SDS ⁄ PAGE. (B) AmyQ secre-tion into the growth medium of B. subtilis as determined by west-ern blotting using the proteins from...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... CD163. In humans and animals, infections or in ammatorystimuli cause changes in the activities and expressionlevels of various forms of CYP in the liver. In mostcases, CYPs and their activities ... he-patocytes [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms [23]. Activated KCs cause oxidativestress on the surrounding tissue, ... GdCl3pretreatment also prevented lipidperoxidation and a decrease in hepatic GSH ⁄ GSSGratio during sepsis. Thus, the results of the presentstudy suggest that ROS produced by KCs mediate the sepsis-induced...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
... importance of these interactions remains unclear and needs to be investigated in future studies.Concluding remarksH. pylori represents one of the most successful humanpathogens, inducing severe clinical ... lengthrestrictions of this minireview. The work of S. W. wassupported by the Deutsche Forschungsgemeinschaft(We2843 ⁄ 2-1) and BGAG-Stiftung Walter Hesselbach. The work of S. B. is supported by grants ... pylori strains encode the cytotoxin-associated gene (cag)-pathoge-nicity island, which expresses a type IV secretion system (T4SS). This T4SSforms a syringe-like pilus structure for the injection...
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Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt
... cotransfected pRL-SV40 control plasmid.StatisticsResults are expressed as the mean ± SD for each sample.Statistical analysis was performed using the unpairedStudent s t -test. Statistical significance ... LD inhibited the increase in Cer levels induced by serum starvationstress. These findings suggest that regulation of Cerlevels is involved in enhanced cell survival resultingfrom activation of ... role in apoptosis and cell-cycle arrest induced by various stressors.Intracellular Cer levels are adjusted by several sphingo-lipid production pathways, such as de novo ceramidesynthesis by...
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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity pdf
... lysates confirmed the absence of cathepsin D in nontransfected cells, and the presence of both the 31 kDa mature cathepsin D and the 45 kDa procathepsin D in cathepsin D-overexpressingcells (Fig. 6A, ... whoseendogenous cathepsin D expression was inhibited by small RNA-mediated gene silencing (cathepsin DsiRNA) (Fig. 6B). A progressive decrease in expres-sion of both procathepsin D and mature cathepsin ... whichmodification of the expression levels of cathepsin D, either by transfection of the cathepsin D gene or small interfering RNA, was followed by parallelchanges in the cytotoxic response to cholera toxin....
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Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc
... isinvolved in cell survival through downstream signalingcascades such as the MAP kinase and phosphatidyl-inositol-3¢-OH kinase (PtdIns3-K) ⁄ Akt pathways.These signals in uence survival through several ... Becausecaspase 9 is intimately associated with the initiation of apoptosis, EGF seems to exert its protective actionagainst ADR-induced apoptosis by suppressingcaspase 9 activity via stabilization ... apoptosissuggests that maximal protection may have requirednew protein synthesis. Several recent studies indicatethat certain growth factors can suppress apoptosis by modulating the process of apoptosis...
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Tài liệu Báo cáo khoa học: Insulin-dependent phosphorylation of DPP IV in liver Evidence for a role of compartmentalized c-Src ppt
... tyrosine-phosphorylated proteins first. Weshow here that one of these proteins, DPP IV, is tyro-sine-phosphorylated in a ligand-dependent manner.ResultsMS analysis of major proteins purified by ... overproduc-tion is associated with attenuated hepatic insulin signal-ing and overexpression of protein- tyrosine phosphatase1B in a fructose-fed hamster model of insulin resistance.J Biol Chem ... B, Simonsson E, Larsson H, Landin-Olsson M,Torgeirsson H, Jansson PA, Sandqvist M, BavenholmP, Efendic S, Eriksson JW, Dickinson S & Holmes D(2002) Inhibition of dipeptidyl peptidase IV...
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Từ khóa: Báo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ