Báo cáo khoa học: Characterization of c-tocopherol methyltra

Báo cáo khoa học: Characterization of c-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana pptx

... SDS-gel electrophoresis of the Blue Sepharose column fraction (Fig. 4A) and agrees with the molecular mass of c-TMT from A. thaliana (Fig. 4B). The presence of only one labelled band is also indicative ... detailed biochemical characterization of a c-tocopherol methyltransferase (c-TMT) from Arabidopsis thaliana and of a c-TMT puriﬁed from Capsicum annuum fru...

Ngày tải lên : 17/03/2014, 09:20

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Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-ﬁxing enzyme in the reductive tricarboxylic acid cycle ppt

... thiosulfatophilum also harbors the same levels of malate dehydrogenase (0.62 UÆmg )1 [1]), previous radiolabeling experiments dem- onstrated a large accumulation of oxaloacetate in the cells, relative ... values from 8.0 to 9.0, and Ches buffers with pH values f rom 8 .7 to 10.0 were used for the assay. RESULTS Isolation of the idh gene from C. limicola In the cell-free extract o...

Ngày tải lên : 08/03/2014, 10:20

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Báo cáo khoa học: Characterization of SCP-2 from Euphorbia lagascae reveals that a single Leu/Met exchange enhances sterol transfer activity pdf

... each lane and analyzed on 16% polyacrylamide gels using Coomassie Blue stain- ing. Lane S1, E. lagascae SCP2 Lys28Glu; lane S2, E. lagascae SCP2 Leu99Met; lane S3, E. lagascae SCP2 Leu13Met ⁄ Leu14Met; ... Leu14Met; lane S4, A. thaliana SCP2 Met100Leu; lane S5, A. thaliana SCP2 Met14Leu ⁄ Met15Leu ⁄ His18Phe; lane S6, E. lagascae SCP2 wild- type; lane S7, A. thaliana SCP2 wild-type; l...

Ngày tải lên : 16/03/2014, 12:20

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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx

... and signal transduction. The role of PS in c-secretase activity is compelling and includes lack of Ab amyloid peptide generation and Notch signalling in PS double knockout cells, cofractionation of ... were obtained from the NHMRC Tissue Resource Centre (Melbourne, Australia) and were well characterized pathologically and clinically [23]. Details of pathology or mutation a...

Ngày tải lên : 16/03/2014, 16:20

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Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redeﬁnition of the I-conotoxin superfamily pot

... well 4. Segments of muscle in wells 2 and 3 were kept submerged by a hold- down pin protruding horizontally from the wall of each well and overlying the muscle. Exposed portions of the muscle ... Bulaj 1 , Elsie C. Jimenez 1,3 and Baldomero M. Olivera 1 1 Department of Biology and 2 Pathology, University of Utah, Salt Lake City, UT 84112, USA 3 Department of Physical Sci...

Ngày tải lên : 16/03/2014, 22:20

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Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... signals were monitored in parallel with cell growth and after cell cycle arrest due to irradiation. Cell proliferation and cell cycle Cell growth behaviour was examined in HeLa and MCF-7 cells. ... from terminal methyl and bulk methylene coupling in ML. The reference cross peak from Lys is also labelled. Label T refers to the glycerol cross peak of triglycerides. The insert show...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... epithelial and conjunctive cells of the mantle. Cg-Clp1 and Cg-Clp2 mRNA levels are increased in haemocytes after bacterial LPS challenges As the two mammalian CLPs, YM1 and YKL-40, were recently ... FEBS Quantiﬁcation of mRNA levels in haemocytes after bacterial LPS challenge In vivo challenge Animals were injected with 100 lg (in 100 lL of NaCl ⁄ P i ) of Escherichia coli 026:...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... QGPI cells 0 1 luc luc luc luc luc luc luc luc luc luc 2 pGL3-Basic Vector −1995H −2081H −1331H −524H −197HmS −94H −73H −60H −45H −14H luc −197H −11871 −11781 Fig. 4. Summary of relative luciferase ... Irrespective of a lack of alteration in levels of other conventional cardiac markers such as creatine kinase isoenzyme MB and cardiac troponin T, serum DNase I levels rose signiﬁcantl...

Ngày tải lên : 19/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... isopropyl thio-b-d-galactoside (IPTG). After 1 h of induction, cells were cooled on ice, centrifuged and stored at )80 °C. Cells pellets were lysed in the presence of 0.4% N-lauroyl sarcosine [30] and ... addition of 0.1% (w ⁄ v) lysozyme and 0.5% (v ⁄ v) Triton X-100 during cell lysis. His 6 -ACA8 1)116 was produced as described by Luoni et al. [31]. Plant material and isolatio...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... homologues) in all kinds of organisms [1]. Extracellular peptidases hydrolyse large proteins into smaller peptides for absorption by the cell, whereas intracellular peptidases play a major role in ... FEBS Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. Atle Noralf Larsen 1 , Elin Moe 1,2 , Ronny Helland 2 , Dag Rune Gjellesvik...

Ngày tải lên : 19/02/2014, 07:20

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