... purchased from Novagen (Madison, WI, USA).Restriction enzymes, modifying enzymes, and PCR enzymeswere from TaKaRa Bio (Kyoto, Japan). Crotalus atroxphosphodiesterase I was purchased from Sigma ... protein agrees with that of other APases[29,33].DiscussionThe SCO2299 protein from S. coelicolorThe SCO2299 gene from S. coelicolor was shown toencode a bifunctional enzyme consisting of an ... are alsofound from genomes of bacteria classified as Actin-omycetales, i.e., Mycobacterium, Thermobifida, Nocar-dia, Corynebacterium and Streptomyces. Among them,the SCO2299 gene from Streptomyces...