Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx

... The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response Andreas Hofmann1, ... structure of Anx (Gh1) (Eur. J. Biochem. 270) 2559 Structure of Anx (Gh1) in comparison with Anx24(Ca32) and AnxA5 The three-dimensional crystal structure of annexin G h1contains the typical annexin ... 2. The membrane binding loops. The IAB loops of Anx (Gh1) (A) and AnxA5 (B) are shown in the same view from the front. (C) and (D) show the IIAB loops of Anx(Gh 1) and AnxA5, r espectively, from...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... Indeed, the enzymestands at a branch point of the tryptophan to NAD pathway, and deter-mines the ﬁnal fate of the amino acid, i.e. transformation into PA, com-plete oxidation through the citric ... Arg47 and the Phe294-Pro295-Leu296 amino acid stretch (Fig. 3A,B). The ligand binds in an extended conformation, with itsphosphate moiety located in the proximity of the zincion and with the ... ª 2009 The Authors Journal compilation ª 2009 FEBSmechanisms proposed for ACMSD [21], the hA-CMSD:DHAP complex represents the ﬁrst structure of an ACMSD in a ligand-bound form and can be usedto...

Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

... showedthat the upper band consisted of the a, b and c subunits in amolar ratio of 1.0 : 1.0 : 1.2. The lower band was composed of the a and c subunits in a molar ratio of 1.0 : 0.9. When the puriﬁed ... root-mean-square (rms) deviations of 0.006 A˚ from the idealbond length and 1.30° from ideal bond angles. The resultingRwork and Rfreewere 0.208 and 0.248, respectively, in the resolution range ... is also that of the glyceroldehydrataseÆcob(II)alamin complex. The dihedral angle of the northern and southern least-squares planes is 5.5°,indicating that the corrin ring of the glycerol dehydratase-bound...

Báo cáo khoa học: The crystal structure of NlpI A prokaryotic tetratricopeptide repeat protein with a globular fold potx

... removing the need for reducing agents during puriﬁcation and handling. We therefore cloned and expressed NlpI, and determined the protein structure by X-ray crys-tallography. The structure reveals ... seen. The AB and AA¢Wpacking angles are responsible for curvature and superhelicity of the motif. (B) Amino acid sequence of NlpI from the translation of the nlpI gene [22], including the signalprosequence ... an unusual association with the next pair of helices,10 and 11. These pack against 8 and 9 at an angle of 96° (visible in Fig. 2D), which is the highest inter-repeat rotation angle within the...

Tài liệu Báo cáo khoa học: The solution structure of reduced dimeric copper zinc superoxide dismutase doc

... distance of 2.6 A˚betweendonor and acceptor, t he N-H-O angle larger than 140° and occurrence in at least 50% of the conformers). The quality of the structure has been estimated byRamachandran ... to play an important role in building the Greek key structure and in designing the metal binding site and the active cavity of the system. The analysis of the H-bonds in the present structure ... 2. The H-bond connecting loop III and loop V,containing b hairpin (HN of Leu38 and CO of Gly93) and the H-bond between HN of Gly93 and CO of Asp90 (loopFig. 7. View of the active channel of...

Tài liệu Báo cáo khoa học: The intracellular region of the Notch ligand Jagged-1 gains partial structure upon binding to synthetic membranes docx

... lack of globular structure, and of little, if any,secondary structure [15]. The presence of strong and sharp resonances accompanied by much weaker peaksin the 1H-15N HSQC spectrum, and the ... expressed and puriﬁed a recombinantprotein starting at the putative intramembrane cleav-age site and comprising part of the transmembranesegment and the entire intracellular region of humanJagged-1 ... shown). The lack of signiﬁcantchemical shift dispersion in the HN and Ha chemicalshifts is an evidence of lack of tertiary structure. On the other hand, NMR spectra suggest that the confor-mation...

Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf

... establish the preferred geometry of the precursor. We now report the chemical synthesis, bio-chemical production, and solution structure of preCbnB2, and compare it with the structure of the maturebacteriocin, ... of eight scans. The bandwidth was set at 1.0 nm and the sensitivity at 50 mdegrees, and the response time was 0.25 s.In all cases, baseline scans of aqueous buffer were subtracted from the experimental ... determine the structural basis of the inhibition of the antimicrobial activity of CbnB2 by the leader and to assist future analysis of the interaction of preCbnB2 with its ABC transporter protease, it...

Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt

... comparison of the effects of roughandsmoothLPSsofE. coli, both in the presence and absence of EDTA (Fig. 1). The rate of oxidation wasincreased in the presence of the LPSs of both the smooth and rough ... enhanced in the presence of Hb. The effect of Hb on the LPS and purified lipid A of roughE. coli has been recently investigated, and significantphysical changes in the purified lipid A and in the ... increased the initial fast phase of the reaction, but decreased the rate of the slow phase of oxidation in the presence of EDTA.A comparison of rough and smooth LPSs of E. coli and S. minnesota in the...

Báo cáo khoa học: Monomeric solution structure of the helicase-binding domain of Escherichia coli DnaG primase pdf

... a continu-ous strand [1]. During DNA replication, a helicase sep-arates the double-stranded DNA into single strands, and replication of the leading strand and synthesis of the Okazaki fragments ... difﬁculties and the discrepancies between the structure of P16 (which is monomeric in solution) and the different conformers in the crystal structure of DnaG-C, we here report the solution structure of E. ... predicted for the corresponding domain from B. stearothermophilus[10] and for the monomeric DnaG-C structure repor-ted here. These data and the uniformity of the relaxa-tion rates along the amino...

Báo cáo khoa học: "The clinical value of daily routine chest radiographs in a mixed medical–surgical intensive care unit is low"

... drafting the manuscript.MG participated in analysis and interpretation of the data and in drafting the manuscript. PS, JS and MV contributed to the conception and design of the study and manuscript ... in the design and statistical analysis of the study. MS conceived and coordinated the study and wasinvolved in the interpretation of the data and manuscript revi-sion. All authors read and ... four of us (MG,GC, EW and MS) carefully read the medical records, checked the patient data management system (Metavision, iMDsoft,Sassenheim, The Netherlands) and searched the hospitalinformation...

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