Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf
... molecules
Fig. 9. The proposed mechanism of catalysis of the b-aryl ether cleavage enzyme.
Fig. 8. Mass spectra of guaiacylglycerol and guaiacol, products of the b-aryl ether cleavage reaction. The reaction ... 2003
Detection and characterization of a novel extracellular fungal enzyme
that catalyzes the specific and hydrolytic cl...
... rep-
resents the first example of the effects of an antimicro-
bial peptide from frog skin on the proteome of
bacteria, and demonstrates that the bacterial mem-
branes are the major targets of its mechanism ... Diagnostica
Molecolare Avanzata, II Facolta
`
di Medicina
e Chirurgia, Azienda Ospedaliera S. Andrea,
via di Grottarossa, 1035-00189 Roma, Italy
Fax: +39 06 337766...
... BC1535-For (5¢-AT
GAAATTAAAAATAGGTATTACAT-3¢) and BC1535-
Rev (5¢-TTAAATCTTTCCATTTTTGTCATC-3¢)forbc1535,
BC3725-For (5¢-ATGTTAAACAAGTTCAAATTTTTTT-3¢)
and BC3725-Rev (5¢-TTATTTTTGCAAGGAAAGACC
AT-3¢) for ... R140E-Rev (5¢-ATAAAAA
GACTCCAC
CTCATGTGGTGAA-3¢) for point mutation
R140E; A4 2S-For (5¢-TTAACAGAA
TCTGATCTTTC
TTCAAAT-3¢) and A4 2S-Rev (5¢-TTTGAAGAA
AGA
TCAGATTCTGTTAAA-3¢) for po...
... four
113
Cd
2+
ions that are known
to bind to the a domain of mammalian metallothion-
ein in a tetrahedral coordination to four thiolate
ligands. The relative assignment of these peaks to the
four cadmium ... Canada) to a final size of 1 K·.
Acknowledgements
We thank Natural Sciences and Engineering Research
Council of Canada for financial support through oper-
ating an...
... the target
Z
I3 1A
gene was amplified by PCR with primers 5¢-AAATA
TAAAACGCTAGCGTCGACATGGCGC-3¢ and 5¢-AGC
GTAAAGGATGGGGAAAG-3¢. The final ratio of target
cells was determined by counting the number ... Izawa K, Matsumura S, Wakamura K, Tanino
T, Tanaka T, Ogino C, Fukuda H & Kondo A (2009)
A simple and immediate method for simultaneously
evaluating expression level and p...
... Ohishi K, Fukasawa M, Shikata
K, Kawai H, Itakura H, Hatanaka M, Sakakibara R,
Ishiguro M, Nakata M et al. (1995) Oligomannose-
coated liposomes as an adjuvant for the induction of
cell-mediated immunity. ... carriers and adult
T-cell leukemia ⁄ lymphoma patients. Leukemia 23, 375–
382.
8 Ohashi T, Hanabuchi S, Kato H, Tateno H,
Takemura F, Tsukahara T, Koya Y, Hasegawa A,
Masuda T &a...
... solutions are compli-
cated, consist of several stages and hand-
built features, and are too slow to be applied
as part of real applications that require such
semantic labels, partly because of their ... predicted by a parser, such as Char-
niak’s parser (Charniak, 2000). Their aim is, given
a node in the parse tree, to assign a semantic role
label to the words that ar...
... (TaKaRa); Lane 2, CybS subunit. The
FP band is not very clear in the agarose gel,
and the longest band was used to run
another PCR reaction for amplification.
X. Huo et al. Characterization of respiratory ... Tsukihara T, Aoyama H, Yamashita E, Tomizaki T,
Yamaguchi H, Shinzawa-Itoh K, Nakashima R, Yaono
R & Yoshikawa S (1995) Structures of metal sites of
oxidized bovine he...
... issue
in the isolation, analysis and chemical synthesis of these
peptides.
Post-translational modifications of neuronally
active a- conotoxins
A feature of conotoxins in general is that they are relatively
richly ... be applicable to synthesis
of a- conopeptides.
Synthesis of variants of a- conotoxins: alanine scans
and loop replacements
In addition to replication of...
... from
manual speaker role labels and manual LUC annota-
tions when available, denoted Manual Annotation, and
automatic LUC annotations and automatically detected
speaker roles, denoted Automatic Annotation.
Agreement
P ... speaker
role labels and manual LUC annotations when man-
ual annotations are available. Table 1 showed that
running a fully automatic system to generate auto-
ma...