... domains or putative
GPI unit, if one exists in CLAP as it does in Drosophila
fasciclin I and many other fasciclin-containing proteins
[36,46]. Fas I domains in proteins are a lmost always found
in ... domains [37]. Of importance to understanding
the structure and function of CLAP, is that most proteins
containing Fas I domains are anchored to cell membranes
through a GPI unit...
... oniae and E. coli infection in
humans [49,50].
Binding of Cry1Ac to proteins of 68-kDa in size in ligand
blots of H. virescens BBMV has been reported previously
[1,5,11]. Our ligand blotting and ... glycoproteins that are part of the
C binding site group and r esistance to C ry1Ac in the
H. virescens YHD2 strain [11].
Cry1 toxin-binding proteins of 60- to 80-kDa in...
... pos-
sess solanesyl and decaprenyl diphosphate synthases as the determining
enzyme for a type of ubiquinone. Here we show that murine and human
solanesyl and decaprenyl diphosphate synthases ... 5609
investigate mammalian long-chain trans-polyprenyl
diphosphate synthases.
Here we describe the identification and characteriza-
tion of solanesyl and decaprenyl...
... range of pH from 3 to 9
(not shown). The effect of staurosporine, a potent pro-
tein kinase inhibitor was also examined. Pre-incubation
of inhibitor with StkP inhibited its kinase activity in a
dose-dependent ... mutant
strains is S1 ribosomal protein involved in RNA bind-
ing. Phosphorylation of this protein on serine residue
was described in E. coli [22] and C. glutamicum [1...
... reciprocal plots yielded lines
converging to the left of the ordinate axis. Secondary plots
of V
)1
intercepts and of slopes were linear for either of the
substrates. These results indicated that the ... earlier findings of
d’Harlingue and Camara [15] and may be, indeed, due to
the tendency of membrane proteins to aggregate. This
aggregation phenomenon may also explain the in...
... AM,
van Lint J & Burgering BM (1998) Essential role for
protein kinase B (PKB) in insulin-induced glycogen
synthase kinase 3 inactivation. Characterization of
dominant-negative mutant of PKB. ... S6 kinase; PDK1, phosphoinositide-dependent protein kinase-1; PKA, protein kinase A; PKB, protein kinase B; PKC,
protein kinase C; p-Ser, phospho-serine; PtdIns3K, phosphatidylinositol 3...
... 1343
where the relevant increase of m
2
produces the relevant
intensity changes of ML ⁄ TG signals. In principle, such
an increase can be due to both a decrease in c
1
and an
increase in p
1
after growth ... sine function was
applied in both dimensions of the time domain before
Fourier transformation.
Integration of 1D and 2D signals was performed using
1D winnmr and 2D...
... and storage of protein
Optimal growth conditions were standardized in order to
obtain the highest amount of total soluble protein, as
assessed by the intensity of a 60 kDa band on SDS/
PAGE and ... Underlining indicates insertion typical
of plant and trypanosomatid i-PGAMs. The amino acids involved in chelation of metal ions are indicated with a circle d: 1, corresponding...
... findings
argue for an early evolutionary origin and a high con-
servation of this class of proteins at both the structural
and functional levels. Given the multiplicity of CLPs
in humans and insects ... transcripts during
development and in adult tissues
To gain insights into possible physiological functions
of Cg-Clp2, determination of its tissue distribution and
tem...
... indicate intron 6 of 75 bp in fragments
B and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and
3¢ portion of 25 bp in intron 6 in fragments F and I. The number at
the right of each RT-PCR ... results in no generation of protein isoforms.
The 5¢-UTR of eukaryotic mRNA in uences the initi-
ation step of protein synthesis and thereby in part
det...