... are
endothelial cells from the vein of human umbilical
cord.
Flow cytometry of endothelial cells
The results of flow cytometry (Fig. 2) revealed that the
HUVECs showed constitutive expression of ... and several of the compo-
nents of the neuronal cholinergic system are not only
Keywords
acetylcholinesterase; biochemical
characterization; cellular membrane; human
e...
... 2006 FEBS
Biochemical characterization of human
3-methylglutaconyl-CoA hydratase and its role in leucine
metabolism
Matthias Mack
1
, Ute Schniegler-Mattox
2
, Verena Peters
3
, Georg F. Hoffmann
3
, ... group of the CoA-acceptor (R
2
-COO
–
).
In the case of glutaconate CoA-transferase from A. fermentans,
CoAS
–
transiently is bound to the c-carboxyl group of bE54 [24].
M. Ma...
... of the enzyme for that cofactor and the
apparent absence of a redox process as part of the catalytic
cycle. The substrate binding site of the type II enzyme
remains veiled. However, a patch of ... influence of substrates and cofactors
on the quaternary structure of the enzyme, we performed
boundary sedimentation experiments under different experi-
mental conditions. In the absenc...
... specificity and enzymatic properties
of USP7
As part of the characterization of USP7 biochemical
properties, we have measured its kinetic parameters for
the hydrolysis of ubiquitin C-terminal 7-amido-4-meth-
ylcoumarin ... molar ratio of
100 : 1 in a final volume of 150 lL. Reactions were stopped
by addition of one volume of 2 · Laemmli sample buffer,
followed by boiling at...
... reduction of
flavin [13,14], and the transfer of electrons to ubiqui-
none, which is part of the respiratory chain. Because
of this connection, pyrimidine formation requires a
sufficient concentration of ... identified as a transmembrane
domain of 17 amino acids in rat DHODH [12], can be
presumed to be a membrane- spanning a-helix. Here,
we were able to predict a transmembrane domai...
... the surface of endothelial cells [4], annex-
in A1 has been observed to bind to an extracellular
domain of b
2
integrin [5]. Annexin A5 was found to
bind to the intracellular part of the b
5
integrin ... Winter et al. Biochemical characterization of annexin B1
FEBS Journal 273 (2006) 3238–3247 ª 2006 The Authors Journal compilation ª 2006 FEBS 3247
Biochemical characterizatio...
... Authors Journal compilation ª 2007 FEBS 1201
Biochemical characterization of rice trehalose-6-phosphate
phosphatases supports distinctive functions of these plant
enzymes
Shuhei Shima
1,2
, Hirokazu ... function of trehalose
biosynthesis in the regulation of starch biosynthesis
has recently been revealed. Trehalose feeding was
found to induce expression of ApL3, encoding a large...
... content of approximately
5.4% (Table 2). From the overall sugar content
(HPAEC) and the estimated sugar contents of the indi-
vidual glycoforms (MALDI-TOF-MS), the proportions
of glycoforms I ... properties of SPC4.
The absorption spectrum of purified SPC4 was recorded in 50 m
M
sodium acetate pH 5. The inset shows the spectrum of the extract-
ed heme. (B) MALDI-TOF-MS analysis of...
... incorporation of GMP residues into the opposite
3¢-end of the mutant RNA.
A second mutation of the U6 substrate RNA consisted of
the introduction of two additional AMP nucleotides in front
of the oligo(A) ... length of the UMP tails of the substrate RNA,
a decreasing number of distinguishable labeled RNA
products was obtained: three bands in lane 2, two in
Fig. 1. Ionic requ...
... in a 15-fold increase in the yield of the
soluble protein. Pantetheinylation of the ACP in ACP-
CON didomain was verified using MALDI-TOF of a
tryptic fragment of the purified protein (data not
shown). ... MALDI-MS.
Biochemical characterization of the LovB KS-MAT
didomain
Equipped with soluble forms of the LovB KS-MAT
didomain and standalone ACP domains, we assayed
the catalytic...