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Báo cáo khoa học: Atrial natriuretic peptide-dependent photolabeling of a regulatory ATP-binding site on the natriuretic peptide receptor-A pptx

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... pHYDPfproduced a predominant band with an apparentmolecular mass of approximately 56 kDa, which isconsistent with the calculated mass of the His-taggedtranslational product of the corresponding hyd ... 3579characterization and evaluation of HYDJsfromJannaschia sp. CCS1.Characterization of HYDJsTo explore the possible cause of the higher specificactivity for conversion of d,l-p-HPH to N-carbamoyl-p-hydroxyphenylglycine ... DNAsequences flanking the Jannaschia sp. CCS1 HYDJsrevealed an ORF encoding a putative allantoate amido-hydrolase, which is part of the urate catabolic pathwayin many organisms [8]. In fact, by...
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Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... in addition to an N-terminal Nco1 cloning site. The forward o ligomer 5 ¢-TCCGAAACCAGCG GCCGCTTTATCGCGTTA AAAC CGGT GATCAA ACCCC -3¢ and the reverse oligomer 5¢-GTAGGCCTT TGAATTCCTCAAAAAGTGCGGCTCGAT-3¢ ... chain and hides a large amount of the hydrophobic surface area. Surfacearea calculations for the pentamer give a total surfacearea of  81 000 A ˚2with 30% ( 24 000 A ˚2) as con-tact area. ... 5¢-CTTTATTTTCAGGGCGCCATGAAGCGCGCAAGACCGTCTGAA-3¢ and a reverse oligomer 5¢-AGCTCGAATTCG GATCCGGTACCTCAGAAGGTAGACAGCAGAACC-3¢. For derivitization with TMR, a Gly-Gly-Cyssequence was introduced at the C-terminus...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... O-acetylation of sialicacid may change with transformation or other alteration in the environment of the cell [63]. The normal human tissuescontain NeuAc, while malignant tumour cells containO-acetyl ... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crabParatelphusa jacquemontiiMaghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S. Jeya SuriyaDepartment ... [34]. An evaluation of the literature revealed that purification of lectin from the hemolymph of crustaceans was most successful by affinitychromatography as it gave a higher fold of purification andpercentage...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf

Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf

... overlap). The sequence regionupstream o f A F499 is AT-rich and contains typical a rchaealpromoter elements. The sequence AAAGGTTAATATAwas f ound 64 bp upstream of the start codon of AF499; ... 3 bp). The region 81–65 bp upstream of t he start c odon of AF499 was identified asan archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a high le vel of identity ... indicates that only a half reaction is catalyzed whenonly H -S-CoM is pr esent and that a reaction intermediate of the catalytic cycle is trapped [6]. Variable-temperaturemagnetic circular dichroism...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... DR2:5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCGTAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAAGGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGGTCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCAAGGTCATGACCTCG-3¢. One strand of each oligonucleotide wasannealed after incubation at 100 °C for ... 21-day worms. SmNR1 contains an autonomous transacti-vation function (AF1) in the A ⁄ B domain as demonstrated in a yeastone-hybrid assay; it interacts with SmRXR1 in a yeast two-hybrid assayand ... 5¢-AAGATGGTATTGAAGATGATGGTTGA-3¢), purified from agarose gelusing Gel Extraction kit (Qiagen, Valencia, CA, USA) andrandomly labeled with32P using a Metaprime kit (Amer-sham Pharmacia Biotech...
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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... standard assay mixtures, the velocity of ABTS oxidationwas maximal at 45 °C. The dependence of the rate of ABTSoxidation by lac1 on substrate concentration at pH 3.0 and45 °C followed Michaelis–Menten ... eightseparate stages were identified as follows: half-colonization of substrate (day 4), full-colonization (day 8), formation of primordia (day 12), appearance of pinheads (day 14), buttonstage (day ... that product abundance was evaluated in the exponential phase of the reaction (half of the maximumproduct). To further validate the assays, the optimized cyclenumber (23 and 28 for gpd and lac1,...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

... primer, 5¢-CATGCCATGGCCAGTAGTCAGCCTGACCCTACTCCAG-3¢ and 3¢ primer, 5¢-GGTTATCATATAAAGATCTCAAATTACCC-3¢, for the second PCR the primers were: 5¢ primer, 5¢-GGGTAATTTGAGATCTTTATATGATAACC-3¢ and 3¢ ... medium was obtained.Reduction and alkylation of SSASSA after Ni–NTA purification was reduced with 10 mMDTT for 2 h at 25 °C. Solid iodoacetamide was then addedand alkylation was allowed to ... next 18 h and thenharvested onto glass fibre filters. Incorporation of radio-activity was then measured using a Liquid ScintillationAnalyzer 1600 TR (Packard, Canberra, Australia). Allmeasurements...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... regulation of caspases. Takentogether, these results indicate that VBARP is a novel splice variant of ANKHD1 and may play a role in cellular apoptosis (antiapoptotic) andcell survival pathway(s).AbbreviationsANK, ... VBARP-L consisted of a 576-bp fragmentabsent from the shorter VBARP-S and contained a 114-bp UTR (Fig. 2A) . Intron and exon analysis of VBARP revealed that VBARP-L and VBARP-S con-tain 11 and ... interaction.Based on these results we were able to demonstrate the formation of a complex between Vpr and VBARP.Characterization of VBARP variants The two VBARP clones differed only in the 5¢-endwhere...
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Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx

Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx

... the 5¢-end of the ORF andcontained a SalIsite(5¢-CTACGTCGACGATGGCGAACATCTGGCCACGAATC-3¢); the reverse primer corres-ponded to the 3¢-end of the ORF and contained an XbaIsite(5¢-AGGCTCTAGATTTATGAGAAATTGGCTTTCTGGAC-3¢).Expression ... last larval stadium, butit gradually increased, reaching a peak at the wanderingstage late in the last larval stadium. At the prepupal stage,EH activity declined to a level equal to that in the ... 5, 8and 14 h post-treatment. The poly (A) -RNA was extracted from the larvae, and 300 ng mRNA of each sample was loaded on a gel. ActinmRNA served as an internal marker to equate mRNA quantities.Fig....
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Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

... conformationalspace of b-aminoacidsislargerthanthatofa-aminoacids, but low-energy conformations of the b-amino acidsbackbone, corresponding to gauche rotamers around the Ca–Cb bond, can overlap canonical ... Ac-b2-HAla-NHMe and Ac-b3-HAla-NHMe indicate that low-energy conformations of the b-amino acids backbone, corresponding to gauche rotamersaround the Ca–Cb bond, may overlap canonical backboneconformers ... potentialenergy surfaces the conformers of b-amino acids that fitwith canonical conformations of a- amino acids, two(/ ) w) Ramachandran maps corresponding to h torsionangle values of +60°,gauche(+),or)60°,...
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