Báo cáo khoa học: Characterization of the Drosophila Methoprene -tolerant gene product Juvenile hormone binding and ligand-dependent gene regulation potx

... FEBS Characterization of the Drosophila Methoprene -tolerant gene product Juvenile hormone binding and ligand-dependent gene regulation Ken Miura, Masahito Oda, Sumiko Makita and Yasuo ChinzeiDepartment of Medical ... activator of gene regulation [45]. Contrary to this, MET exists in the nucleus even in the absence of ligand. Upon ligand binding, it becomes a transcriptional activator. Thisresembles the ligand-dependent ... localization sequence. Therefore, it is reason-able to consider that the GAL4–DBD fusion of METsits on the UAS of the reporter construct even in the absence of ligand, and that the MET moiety is...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... 14-3-3s.Results The C-terminus of isoform 1 of the PM H+-ATPase of A. thaliana (AHA1) interacts with the ﬁrst 88 aminoacids of PPI1 [22], indicating that the PM H+-ATPase binding site of PPI1 is ... stress, and their binding to the H+-ATPase is suggested by the parallel increase of the number of FC -binding sites [18–20]. As to auxin, a soluble auxin receptor hasbeen reported to bind and activate ... cleavage of the enzyme, indicatingthat the PPI1 binding site in the H+-ATPase C-termi-nus is localized upstream of the main tryptic cleavagesite and thus is different from the 14-3-3 binding site....

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... Presumably, their homologues SNO2,SNO3, SNZ2 and SNZ3 complement the defect. The relationship of all of these genes remains to be clarified.Expression and purification of Sno1p and Snz1pIn light of the ... and generated by the product of gene pdxA, is not involved,compounds related to it structurally could be the couplingpartner of the ketopentose in yeast.One characteristic feature of the genes ... depending on the occurrence of a tag and the location of the protein in question, Sno1p, Snz1p and theircomplex were eventually purified to near homogeneity(Fig. 3). Typically, 30, 30 and 25 mg of protein...

Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc

... out in triplicate, and the error bars indicate the standard deviation.Fig. 8. Eﬀects of mutations in the Ets- and GATA -binding sites and the E-box of the mouse a3 integrin gene on promoter activity. ... structures of the mouse a3integrinsubunit gene including the exon/intron organization and the alternative exon usage for the generation of variants of the a3 subunits (a3A and a3B) [18]. In the present ... 324 bp upstream of the major transcription start site. The presence of a CCAATbox and the absence of a TATA box seem to be one of the characteristics of the mouse integrin a3 gene. We identiﬁed...

Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt

... Cleavage of the C9–C10double bond (a) results in products 1 and 2 from I, products 1 and 3 from II, and products 3 and 4 from III. The cleavage of (b) leadsto product 4 from II, and product ... preference of OsCCD1 for the C5¢–C6¢ and C7¢–C8¢ double bonds depends on the nature of the substrate. The cleavage of a sole double bond in the cyclic moi-ety of 3-OH-c-carotene and of monocyclic b-apocarote-nals ... the photosynthetic apparatus fromphoto-oxidation, and represent essential constituents of the light-harvesting and of the reaction center com-plexes [1–4]. Carotenoids are also the source of...

Báo cáo khoa học: Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida) ppt

... kDa, and, unexpectedly, another speciﬁc and even stronger band appeared higher up in the gel(Fig. 1B, lane 6). A subsequent incubation of the gelin DPI resulted in inhibition of the lower band ... arrows).Owing to the horizontal smear, however, the presence of the B14 subunit in the lower band cannot be ascer-tained at this point. A cross-reaction of the antibodyagainst the 39 kDa subunit of bovine ... C˘erma´kova´), the GrantAgency of the Czech Academy of Sciences Z60220518, the Grant Agency of the Czech Republic 204 ⁄ 06 ⁄ 1558 and the Ministry of Education 2B06129 and LC07032(to J. Lukesˇ).References1...

Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx

... footprinting of the 5¢upstream region of the a-F1-ATPase gene and found that the combination of GAF and Adf-1 binding sites is presentboth in o ther Drosophila species (D. yakuba and D. pseu-doobscura)andinAnopheles ... severaltranscription factors and coactivators that regulate the expression of genes playing key r oles in the biogenesis of the OXPHOS system. In general, the transcription of nucleargenes encoding proteins ... in und erstanding the transcriptional regulation of genes encoding essential components of the mitochondrial respiratory chain in Drosophila melanogaster. The energetic demands of the different...

Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot

... trans-ferase that synthesizes (p)ppGpp. Production of thisalarmone results in a down -regulation of stable RNAsynthesis (rRNA and tRNA) and up -regulation of the synthesis of mRNAs encoding enzymes ... 6E).From these results it can be concluded that tRNAswere bound in classical states with the tRNAMetfboundin the P-sites of the 30S and 50S subunits and the tRNAPhebound in the A-site of the ... encoded by the relAlocus in Escherichia coli. We have cloned and puriﬁedSF from E. coli and examined the ribosome, tem-plate and tRNA-dependence of the pppGpp synthesisreaction.Puriﬁcation of recombinant...

Báo cáo khoa học: Characterization of the bioactive conformation of the C-terminal tripeptide Gly-Leu-Met-NH2 of substance P using [3-prolinoleucine10]SP analogues pdf

... methanol [35]. The random coil value of Pro was used for the different prolinoamino acids. Therefore, the CSDs of P3Leu10 depend primarily on the chemical modification of the pyrrolidine ring and cannot ... and EC50values for the cAMP and IPs pathways, and arereported in Table 1 [20,28]. The affinity of the transanalogue [Pt3Leu10]SP is similar to that of SP and [Pro9]SPat both binding sites. The EC50values ... structure. The confor-mations of residues 8–9 are ill defined in the peptides. Thus, the introduction of a prolinoamino acid in the C-terminaltail of SP does not affect the overall structure of the...

Báo cáo khoa học: Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli pptx

... composed of multiple species with the N-termini of Asn43, Val46 and Ile56, and that at a large dose (lane 6) consisted of a single species with the N-terminus of Ile56(Table 1). The N-terminus of the ... propeptide of subtilisin adopts an arranged structure only in the presence of the mature form of the protease [23].Whether or not a similar mechanism is responsible for the folding of the glutamyl ... inhibited. The a-amino group of the N-terminus of shorter propeptides may functionas a weak acceptor of the negative charge of a sub-strate peptide.In the present study, the role of Val69 was...

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