Báo cáo khoa học: Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress pdf

$Báo cáo khoa học: Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress pdf$

Báo cáo khoa học: Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress pdf

... assessed in con-nection with alterations in photochemical activity dur-ing strong light illumination of the PSI submembrane fractions. It is concluded from the speciﬁcity of thevarious active oxygen ... FEBS Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress Subramanyam Rajagopal*, David Joly, ... strong light illumination. It declined withfurther light exposure, with only 30% of its initialmagnitude remaining after a 3-h illumination. Similarto the absorbance spectra, a blue shift of...

Báo cáo khoa học: Protective effect of dietary curcumin and capsaicin on induced oxidation of low-density lipoprotein, iron-induced hepatotoxicity and carrageenan-induced inﬂammation in experimental rats ppt

... combination on iron-induced LDLoxidation in vivo and copper-induced LDLoxidation in vitroOxidation of LDL observed in iron(II) sulfate-injectedrats as measured by thiobarbituric acid reactive ... oxi-dation induced in vivo by the iron(II) ion caused anincrease in the anodic mobility of LDL in the case of control rats (Fig. 1). In the case of animals maintained on curcumin, capsaicin ... their combina-tion on the susceptibility of low-density lipoprotein (LDL) to oxidationwas examined in an animal study. Individually, both dietary curcumin and capsaicin signiﬁcantly inhibited...

Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt

... electron density in the lysine ligand loop is revealed, leadingto residues K97 to T101 being modeled with a double main-chain confor-mation. Upon unfolding, dissociation of the lysine ligand ... coordination. Upon titrating increasing amounts of guanidinium hydrochloride (GdmHCl) the protein unfolds resulting in the perturbation of a number of electronic absorption bands and the appearance in ... chain in both proteins canadopt two conformations stabilized by H-bond inter-actions. Finally, a change in the side-chain orientation of F102 is observed. In the wt model the ring is planarwith...

Báo cáo khoa học: The effect of zinc oxide nanoparticles on the structure of the periplasmic domain of the Vibrio cholerae ToxR protein pot

... regulation of protein protein interactions,cellular signal transduction and transcription of DNA[4–7]. Conformational transition, leading to peptideaggregation and the formation of amyloid fibrils, ... amyloid b protein (Ab)[8,9]. Various specific and nonspecific interactions,Keywordsnanoparticle protein interaction; protein unfolding by nanoparticle; ToxR protein; Vibrio cholerae; zinc oxide ... Recognit 12, 3–18.46 Connelly PR (1994) Acquisition and use of calorimetricdata for prediction of the thermodynamics of ligand-binding and folding reactions of proteins. Curr OpinBiotechnol...

Báo cáo khoa học: The effect of ST2 gene product on anchorage-independent growth of a glioblastoma cell line, T98G doc

... mechanisms of their action arestill unclear.The expression of cytokines and their receptors in thebrain prompted us to investigate ST2 expression and itspossible implications in brain tumors. ... autocrine mechanism for the suppres-sion of colony formation (Fig. 5).Finally, we confirmed the inhibitory effect of the ST2 protein on colony formation in soft agar by addingexogenous purified ... efficiency of colonyformation in soft agar was significantly decreased in thecase of cells that continuously produce the ST2 protein. Furthermore, inhibition of colony formation in soft agarwas...

Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-speciﬁc truncated splice variant Mark Wehner and Christian Herrmann doc

... changes in confor-mational flexibility imposed by nucleotide binding,while in the case of hGBP5, conformational restrictionsafter nucleotide binding and oligomer formation appearto result in strong ... dur-ing changes in the oligomerization state. We observed only a minor in u-ence of the C-terminal truncation on hydrolysis, nucleotide binding and oligomerization of hGBP5. Based on these similarities ... three-fold increase in specific activity (see Fig. S1). Both splicevariants exhibited positive cooperativity in specificactivity, with Hill coefficients of 2.4 and 1.9 and dissoci-ation constants...

Báo cáo khoa học: Multiple effects of DiS-C3(5) on mitochondrial structure and function pot

... low concentrations preven-ted progression of intrinsic oxygen consumption bymitochondria. However, this effect of DiS-C3(5) did notreﬂect inhibition of the mitochondrial respiratory chain, ... novel effects of DiS-C3(5) on mitochondria: (i) inhibition of the Ca2+-induced PT by a low concentration of DiS-C3(5) and (ii)induction of swelling in the absence of P i .Withrespecttothe ... concentra-tions on the progression of intrinsic oxygen consumptionby mitochondria might reﬂect induction of the PT byendogenous Ca2+. So next we examined the validity of this interpretation.First,...

Báo cáo khoa học: Differential effects of histone deacetylase inhibitors on phorbol ester- and TGF-b1 induced murine tissue inhibitor of metalloproteinases-1 gene expression docx

... and subsequent protein stabilization [37,38]. The proteo-some inhibitor lactacystin inhibited the PMA induction of Timp-1 and no induction was seen in the additionalpresence of TSA (data not ... expression of Timp-1, only on induced gene expression. In conclusion, this is to our knowledge, the onlydescribed instance of HDAC inhibitors having oppos-ite effects on the same gene, depending ... FEBSHDAC inhibitors and Timp-1 expression D. A. Young et al.Differential effects of histone deacetylase inhibitors on phorbol ester- and TGF-b1 induced murine tissue inhibitor of metalloproteinases-1...

Báo cáo khoa học: The heterogeneity of mast cell tryptase from human lung and skin Differences in size, charge and substrate afﬁnity ppt

... a and bI, and at the second locus between bII and bIII, would give rise to nine possible genotypes (aabIIbII,aabIIbIII, aabIIIbIII, abIbIIbII, abIbIIbIII, abIbIIIbIII,bIbIbIIbII, bIbIbIIbIII, ... larger and more intense than those for dimerictryptase. There was in all cases a corresponding reduction in band size and staining intensity with increasing degree of oligomerization, so that in ... Partial glycosy-lation of antithrombin III asparagines-135 is caused by the serine in the third position of its N-glycosylation consensus sequence and is responsible for production of the b-antithrombin...

Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx

... rRNA, rendering theribosome unable to bind elongation factors and henceabolishing protein synthesis [3]. The site of depurinationby RTA lies within a highly conserved purine-rich region of 28S ... Trilux liquid scintillationcounter.ResultsN-glycosidase activity of RTA variants containingarginine substitutionsRTA variants in which arginyl residues 213 or 258 weresubstituted with Ala, ... challenged withincreasing concentrations of toxin at 37 °C for 4 h. Remaining protein synthesis after this time was measured by the incorporation of (35S)methionine. Symbols indicate the mean of four...

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