Báo cáo khoa học: Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus ppt
Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot
... [8]. The heterodimeric complex of Hop2 and Mnd1 stimulates strand exchange of Lim15/Dmc1[9–11]. The meiosis-specific proteins Mei5 and Sae3form a complex with Lim15/Dmc1 and are necessaryfor the ... to the site of the Lim15/Dmc1–PCNA complex and deposits histone H3 (or a histone vari-ant) and H4 on the naked DNA to restore the nucleosome structure (Fig. 4C). Because of the vari-ous interactions ... activity and invades the homologous double-stranded region of the other allele. These steps of homology search and recombination are catalysedby two bacterial RecA homologues, Rad51 and Lim15/Dmc1....
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Báo cáo khoa học: Estrogen-related receptor a and PGC-1-related coactivator constitute a novel complex mediating the biogenesis of functional mitochondria potx
... FTC-133, XTC.UC1 and RO 82 W-1, the expression of PRC and ERRa was correlated with the mitochondrial mass, the expression of mitochon-drial genes and the activity of the COX and CSenzymes. ERRa ... involved in the metabolic regulation of the three thyroid cell linesWe investigated the effects of XCT790, a specificinverse agonist of ERRa. As controls of the inhibitoryeffect of XCT790 on ... of the cells. Nevertheless, the inhibition of ERRa with XCT790 decreased significantly the basaloxygen consumption and the maximal respiration onlyin FTC-133 cells (Fig. 2B). Moreover, COX and...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... and enzyme–product complexes [29]. On the other hand,kcat⁄ Kmis an apparent second-order rate constant thatrefers to the properties and the reaction of the freeenzyme and free substrate [29]. We have therefore ... published elsewhere. We havetherefore measured the thermodynamic parameters of RNase-A in the presence of these amino acids and amino acid derivatives, and values of DGD°, measuredin triplicate, ... decreased. On the otherhand, this change in conformation results in a decreasein kcat, the turnover number of the enzyme in the pres-ence of sugars, i.e. the maximum number of substratemolecules...
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Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant pdf
... in the side chainconformation of this residue. The shift of the Ca atom of Tyr35 of the Phe35fiTyr mutant from that of Phe35 of the wild-type cyt b5is 0.21 A˚, within the error limit. The ... [35,36,40,41]. The apo-cyt b5 and apo-Mb have no absorption in the Soret bandregion because of the lack of the heme prosthetic group. For the holo-cyt b5 and holo-Mb, the CD spectra of the Soretband ... lines. Phe35 and the hemegroup of wild-type cyt b5,shownasthinlines,are superimposed with Tyr35 and the heme of the mutant. (B) Tyr35 of the Phe35fiTyrmutant is superimposed with Phe35 of the wild-type...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... KIND2 and 43 residues of the mouse MAP2 central domain. ThreeLeu residues at amino acid positions 461, 474 and 477 in the MAP2-bindingcore module of KIND2 contributed to the interaction. The ... localized to the N-terminal region, and theirspecific functional domains are located in the C-termi-nal region. The C-lobe of protein kinases mediates the interaction with activators, substrates and ... in the cell lysates.Taken together, these data indicate that the MAP2-binding core site of v-KIND is sufficient for the v-KIND-MAP2 interaction within cells. The MAP2-binding core module of...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... appearance of an additional peak at 7.65 mLon the elution profile. Increase of the temperature of incubation was accompanied by the simultaneous increase of the peak eluted at 7.65 mL and decrease of the ... 0.05, and 0.15 ± 0.06 lMin the absence of HSP25, and in the presence of the wild type HSP25 and its 3Dmutant, respectively. At the same time, both wild type and especially 3D mutant of HSP25 ... the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actinIn the first series of experiments we analyzed the effect of HSP25 on the apparent critical...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... more than 50% of the GlnK–TnrA interaction in the presence of 2 mm ATP.On the other hand, 2-oxoglutarate did not influence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, ... affect the binding of ATP to GlnK [12]. Therefore, we investigated the binding of TnrA to GlnK in the presence of differentmixtures of Mg2+or Mn2+with the effector moleculesATP and 2-oxoglutarate. ... in the form of the heat effect during the titration of 25 lM GlnK solution (trimer concentration) with ATP (titration from2.1 to 73.5 lM). The lower panels show the binding isotherm and the...
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Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
... Parallel purification of threecatalytic subunits of the protein serine ⁄ threonine phos-phatase 2A family (PP2A(C), PP4(C), and PP6(C)) and analysis of the interaction of PP2A(C) with a4 protein.Protein ... with the C-terminal region of the catalytic subunits of PP2A, PP4 and PP6. The TIPRL-interacting region on the catalytic subunit was mapped to residues 210–309 and does not overlapwith the a4-binding ... residues 106 and 171 to the C-terminus, respec-tively. The interaction between TIPRL and the catalyticsubunit of type 2A phosphatases was verified by re-transforming the prey plasmids into the L40...
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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
... responsible for the actual membranefusion step. However, the nature of the interaction of viral fusion proteins with membranes and the mechan-ism by which these proteins accelerate the formation of membrane ... spectra for the E2 peptides showed the characteristics of a random-coil conformation, asindicated by the presence of a negative band atFig. 1. Fluorescence emission spectra of the E2( 7–26) (black ... peptide–membrane interaction, and com-bined analysis of the data obtained provides insightsinto HGV ⁄ GBV-C–membrane interaction. Results The E2 peptides synthesized are amphiphilic because of the presence...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... mol ⁄mol). The results are mean ± SD, n ¼ 1–4. The degree of permea-bilization is expressed as the percentage of the maximal valueobtained at the end of the assay by the addition of 2 mM TritonX-100. ... (TEM) and atomic forcemicroscopy [15].In this study, we measured the interaction of stefin Bwith various combinations of phospholipid monolayers and bilayers. Interaction of stefin B in the prefibrillaraggregated ... after the end of injection.Discussion The main hypothesis for pathology in AD and otherneurodegenerative diseases is the modified ‘amyloidTable 1. Critical pressures for the insertion of stefins...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnchuyên đề điện xoay chiều theo dạngMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ