Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE pot

Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE pot

Báo cáo khoa học: Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE pot

... transcarbamoylation reaction catalyzed by HypF The formation of the HypE- thiocyanate involves first the carbamoylation of the C -terminal c ysteine of HypE b y interaction with HypF, then the release of HypF and ... future whether they can transfer the carboxamido moiety to the HypC · HypD complex. Analysis of the transcarbamoylation reaction cat...
Ngày tải lên : 16/03/2014, 18:20
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Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc

... mea- sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised for observation by microscopy and FCS ... constants of 11.7, 12.9 and 5.4 lm 2 Æs )1 , respectively, in the part of the cytoplasm outside of the granules. FRAP analysis in the gran- ules provided t...
Ngày tải lên : 18/02/2014, 16:20
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Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt

Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt

... [32]. The Lsm1 and Lsm8 polypeptides are most similar in the regions of the Sm1 and Sm2 motifs. These motifs form the Sm-fold, the hallmark of the Sm-like pro- teins, consisting of a five-stranded ... investigate the requirement for different domains of the Lsm1 and Lsm8 proteins in their function and localization, we created a series of mutant and hybrid...
Ngày tải lên : 07/03/2014, 02:20
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Báo cáo khoa học: Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family pot

Báo cáo khoa học: Analysis of the NADH-dependent retinaldehyde reductase activity of amphioxus retinol dehydrogenase enzymes enhances our understanding of the evolution of the retinol dehydrogenase family pot

... enzymes. The most significant difference between the amphi- oxus and the other retinal reductases was, nevertheless, their preference for the NADH cofactor. To our knowledge, these are the first ... out for 15 min at 37 °Cin 0.5 mL. The amount of protein used in the reaction mixture was 15 lg. The reaction was terminated by the addition of an equal volume of cold...
Ngày tải lên : 07/03/2014, 09:20
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Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot

Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot

... 2A). The binding of raptor to these two variants was similar (Fig. 1D), implying that the loss of raptor binding on removal of the first 17 residues may be accounted for simply by the loss of the ... define the RAIP motif [25]. The use of the rat protein also has the advantage that there is no cross-reactivity of the (P)Ser64 antibody with other sites, which is...
Ngày tải lên : 16/03/2014, 06:20
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Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins potx

Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins potx

... structure found in the large rRNA of prokaryotic and eukaryotic ribosomes [1,2]. At present, they are subdivided on the basis of the structure of the genes and the corresponding proteins into holo-RIPs and ... K. (1987) The mechanism of action of ricin and related toxic lectins on eu- karyotic ribosomes. The site and the characteristics of the mod- ifi...
Ngày tải lên : 23/03/2014, 12:20
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Báo cáo khoa học: Analysis of the stability of the spermadhesin PSP-I ⁄ PSP-II heterodimer Effects of Zn 2+ and acidic pH pdf

Báo cáo khoa học: Analysis of the stability of the spermadhesin PSP-I ⁄ PSP-II heterodimer Effects of Zn 2+ and acidic pH pdf

... [24], and the C1s protease of the C1 complex of complement [25]. Thus, the Trp band can be regarded as a characteristic fingerprint of the native fold of PSP-I and PSP-II. The near-UV CD spectra of ... induced by Zn 2+ was reversed by the addi- tion of EDTA to the sample (Fig. 2A). On the other hand, no significant decrease in the heterodimer stabil- ity wa...
Ngày tải lên : 23/03/2014, 15:21
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Báo cáo khoa học: Analysis of the effect of potato carboxypeptidase inhibitor pro-sequence on the folding of the mature protein pot

Báo cáo khoa học: Analysis of the effect of potato carboxypeptidase inhibitor pro-sequence on the folding of the mature protein pot

... min; then 10 lLofincreasing concentrations of PCI or ProNtPCI were added and the measures were continued for 2 min. The slope of the first part of the assay corresponded to m o and the slope of the ... in vitro and in vivo [7]. There are not many examples of the role of the pro-regions in small disulfide-rich proteins. In these proteins, the folding process...
Ngày tải lên : 23/03/2014, 21:20
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Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx

Báo cáo khoa học: Analysis of the RNA degradosome complex in Vibrio angustum S14 potx

... Band 4 showed more RNase E and PNPase than RhlB and enolase. By contrast, Bands 1, 2, 3 and 5 showed roughly equivalent amounts of each of these proteins in the four samples. Interestingly, Band ... in each band, indicating that the bands excised from the BN-PAGE gel are composed of different proteins. (C) Western blot of the second dimension SDS ⁄ PAGE separation...
Ngày tải lên : 29/03/2014, 21:20
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Báo cáo khoa học: Analysis of the region for receptor binding and triggering of oligomerization on Bacillus thuringiensis Cry1Aa toxin potx

Báo cáo khoa học: Analysis of the region for receptor binding and triggering of oligomerization on Bacillus thuringiensis Cry1Aa toxin potx

... mutant toxins and the analysis of their binding activity to the receptor to investigate the role of mutated amino acid residues; (b) the analysis of the influence of the modification of the cysteine ... osmotic swelling and bursting of the cell, leading to disruption of the midgut tissue and death of the insect [16]. The three-dimensional structur...
Ngày tải lên : 29/03/2014, 22:21
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