Báo cáo khoa học: The optimization of protein secondary structure determination with infrared and circular dichroism spectra docx

Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

... aB-crystallin (i.e. the effect of the additive on the aggregation of the target protein is inde-pendent and different from that of the chaperone). The standard errors associated with this theoretical ... against this target protein. The effect of Arg-HCl on the structure and assembly of aB-crystallinWe investigated whether the effects of these additiveson the chaperone action of aB-crystallin ... calculated from the standard errorsassociated with the means of the measured values (i.e. bycalculating the square root of the sum of the associatedindividual errors squared). The maximum percentage...

Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx

... and the effect of the absence of intact complex I, but the results are toopreliminary in this regard.Heterologous expression of the ESSS-HA subunit The localization of the ESSS subunit in the ... revealed the existence of two additionalsubunits (bringing the total to 46), and one of these, the ESSS protein, is also encoded by an X-linked g ene inhumans and mouse [36]. Most of these subunits ... two of thesemutants the protein i s truncated at the C-terminus of the targeting sequence; the m utants are null mutants for the ESSS subunit. There is no detectable complex I assembly and a...

Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf

... required for efﬁcientcleavage [11]. The solution structure of the SEA domain of the mouse homologue of MUC16 (which in the humanencodes the core protein of the ovarian cancer antigenCA125) was ... MUC16 and MUC17 and other proteins with cleaved SEA domains (Table 1). The SEA module of rat MUC3 is cleaved soon after translation in vivo and in vitro at the Gly-Ser peptide bond of the LSKG ... has little if any effect on the cleavageevent. On the other side of the loop, the valine residue(V189) that interacts only with the C-terminal residues of the b1 strand, also has little impact...

Báo cáo khoa học: The role of Tyr71 in Streptomyces trypsin on the recognition mechanism of structural protein substrates ppt

... substitution of residue 71We measured the CD and ﬂuorescence spectra of SGT-L71Y, SOT-Y71L, SOT and SGT to investigate the effect of the mutation at residue 71 on the tertiary and secondary structures of ... sche-matically. The recombination sites of chimeras A–H are shown in (B). The primarystructures of SOT (upper) and SGT (lower)are shown. The recombination sites of the chimeras are boxed. The names of the ... SOT and SGT. Figure 6Ashows that the CD spectra of SOT-Y71L and SGT-L71Y were drastically different from those of wild-typeSOT and SGT. The spectra of the tryptophan ﬂuores-cence emissions of...

Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt

... nmol or 0.66 nmol of rMIH-B at the arthropodialmembrane of the periopod and returned to the culturetanks. At 24, 48 and 72 h after injection, the hepatopan-creas and ovary of the shrimp were ... females. The percentage indicates the GSI of the females. M (B) indicates the expression pattern of MIH-B in the same tissues in males (N ¼ 5). The lower panel is the northernblot analysis of MeMIH-B ... the tiger prawn Penaeus monodon. As there is a high degree of sequence similarity and structural conservation of these genes and gene products, conﬁrmation of the trueidentity and function of...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... disruption of the association of these proteins could represent a poten-tial therapeutic strategy for the treatment of AD. Here,we used GST fusion proteins of Fyn-SH2 and Fyn-SH3 to further investigate ... wild-type and all of the mutant forms of tau except for YallF and Y394F tau (Fig. 3). In contrast to the results obtained with Fyn-SH2, wild-type tau and all of the YF mutanttau proteins were detected ... between tau and Fyn-SH3, and this furthersuggests that different, and possibly independent, mech-anisms are involved in these interactions of tau with the same protein. Interactions with Fyn-SH3...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... induce a massive accumulation of the reporter protein in the cytoplasm, exceeding that of the reporter protein fused with the full-lengthASF ⁄ SF2 protein, or with the RRM1 alone. Mostlikely, ... upon expression of CP alone. The tethering of TTP led to a decrease of 8MS2 Rluc reporter mRNAto the same extent as that at the protein level(Fig. 7E), therefore conﬁrming the mRNA-destabiliz-ing ... On the other hand, other studies indicated that ASF ⁄ SF2activates the translation of mRNAs bearing ASF ⁄ SF2binding sites within the coding region in intact cells[16,54]. The capacity of...

Tài liệu Báo cáo khoa học: The occurrence of hemocyanin in Hexapoda docx

... that open to the atmosphere on the cuticle and radiate to all parts of the body. O2is delivered through trachea and trache-oles in the gaseous phase [9] and hence respiratoryproteins have ... possible ‘intermediate’. The evolution of hemocyanin subunits follows the widely accepted phylogeny of the Hexapoda and provides strong evidencefor the monophyly of the Polyneoptera (Plecoptera, ... indication of the presence of hemocyanin(data not shown). The notion of the absence of hemo-cyanins from Holometabola is corroborated by the factthat no hemocyanin sequences could be identiﬁedin the...

Tài liệu Báo cáo khoa học: The sequentiallity of nucleosomes in the 30 nm chromatin ﬁbre pptx

... consists of the entire chroma-tin of the nucleated avian erythrocytes and comprisesapproximately 85% of the chromatin in other celltypes [5]. The structure of chicken erythrocyte chromatin is the ... on the relationship with respect tonucleosome orientation and tilt versus chromatinrepeat length. Thus, they differ with respect to the ori-entation of the nucleosomes and the path of the ... evidently come from the ends of the ﬁbre because of the corresponding decrease of the average number of nucleosomes per chain in the main peak. Digestion of the EDC crosslinked samples with MNase produced...

Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... associated with the intercalativeconformation of the (+)-cis adduct and the minor-groove conformation of the (+)-trans adduct, the lat-ter interfering with interactions of the catalytic loops of the ... accordance with this, the depend-ence of the ﬂuorescence of the (+)-trans adduct on itsposition relative to the target dC was revealed in the case of the formation of the complexes of M.SssI with ... effect on the binding of these MTases and on kcat. The minor-groove (+)-trans adductinterferes with the formation of the normal DNA minor-groove contacts with the catalytic loop of the MTases....

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