Báo cáo khóa học: Acharan sulfate, the new glycosaminoglycan fromAchatina fulica Bowdich 1822 Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix docx

... 2004 Acharan sulfate, the new glycosaminoglycan fromAchatina fulica Bowdich 1822 Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix Tuane ... were thenexamined and photographed using a light microscope(Zeiss, Axioskop 2).ResultsHeterogenic forms of acharan sulfate are present in the soft body, mucus and the organic shell matrix The ... observed in the proﬁle obtainedfor GAGs from the organic shell matrix when compared to the other proﬁles, where peaks R3 and R4 predominate over the other R-peaks, which are either present in a...

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

... eggs in the one-cell stage to visualize the localization and to mea-sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the ... of the constructs microinjected into the medaka eggs and transfected into HeLa cells. The olvas, nanos and tudorcoding sequences were joined to the C-terminal region of the GFP or RFP coding ... expressed in the medakaPGC at stage 24 and localized in the nuage. 3D imaging of Olvas–GFP was performed at the indicated times (B). The nuage wasseen around the nucleus and came together and apart...

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... solution in the Dali result. It islikely that the inte rface between N-terminal and b-he licaldomains participate in binding of the polysaccharide,pullulan.Comparison of the active sites of the ... bacteriala-glucosidase (retaining enzyme) and fungal glucoamylaseFig. 3. Overall structure of the model of IPU. N-terminal and b-helicaldomains are shown in light pink and grey, respectively.Theaminoacidresidues ... impossible for the substrate to be retained in the cleft. In the model of IPU,several aromatic and charged residues, Trp277, Tyr349, and Asp371, are p resent in t he v icinity of G ly350, and c ould...

Báo cáo khoa học: "Search in the Lost Sense of “Query”: Question Formulation in Web Search Queries and its Temporal Change" pptx

... building question-answering sys-tems using web knowledge and Wikipedia (see Du-mais et al. (2002) and the references therein). Ourﬁndings call for a greater synergy between QA and IR in the ... context and an improved un-derstanding of question-queries by search engines.2 Related workThere has been some work on studying and exploit-ing linguistic structure in web queries. Spink and Ozmultu ... not on clusteringbut on understanding the expression of intent.3 MethodWe address the main thesis of the work by retrospec-tively studying queries issued to a search engine over the course of...

Báo cáo khoa học: Elements of the C-terminal t peptide of acetylcholinesterase that determine amphiphilicity, homomeric and heteromeric associations, secretion and degradation docx

... AChETsubunits in the ER, and thus facilitate their physiological association with the anchoring proteins, ColQ and PRiMA. Structural differences certainly explain that complexesformed with the T12D andsomearomaticmutantsfolloweda ... mutations in the N-terminal and C-terminal region ﬂanking the aromatic-rich segment.Secretion was increased by mutation E1A and decreasedby mutation Ô4AÕ in the N-terminal region. It was increasedby ... with the collagen, ColQ, or with the transmembrane protein, PRiMA [18,19]; in mammals,these structural proteins anchor the major functional speciesof cholinesterases in the basal lamina of the...

Báo cáo khoa học: Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of b-arrestin 1 recruitment potx

... affectinternalization. Taken together, these data indicate the ability of CRFR1 to internalize in the absence of the majority of putative phosphorylation sites in the C-terminaltail and third intracellular ... receptors in response to CRFR1 activation. However, the possibilityremains that C RFR1 contains determinants of b-arrestin 1interaction apart from the C-terminal tail and/ or serine/threonine in the ... and the broad spectrum kinaseinhibitor staurosporine. Finally, a series of receptors withmutations in the third intracellular loop and C-termin al tailare constructed and examined for their...

Báo cáo khoa học: Insights into the design of a hybrid system between Anabaena ferredoxin-NADP+ reductase and bovine adrenodoxin pot

... the changes introduced in the redox cofactor environment upon complex formation. Theyare therefore related to the specificity and the strength of the protein–protein interaction. Therefore, the ... complex involved, both, long-range electrostatic interactions for the initial protein–proteinencounter and also short-range specific interactions at the protein–protein interface in the optimal ... than within the physiological partners,pointing to a low specificity in the interaction surfaces of the proteins in the hybrid complexes.Keywords: adrenodoxin; electron transfer; ferredoxin-NADP+reductase;...

Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-speciﬁc blood vascular and lymphatic endothelial cells of the mouse pdf

... in the brain, heart, lung, liver and uterus (Fig. 2B), butwas also expressed in Lyve-1-positive lymphatic endo-thelial cells (LECs) in the heart, lung and uterus, and sinusoidal ECs in the ... phosphotyrosine. (D) The uterine BECs and LECs formed tube-likestructures. Bars = 200 lm. (E) SV40-ori-positive plasmids bearing GFP and drug-resistance genes were maintained in the uterine BECs and LECs ... toexamine the endothelial cell biology of the mouse, and will accelerate the molecular and cellular analysis ofECs and their heterogeneity in various vascular beds. The early mortality of the...

Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot

... results and modeling simulations led us to suppose that the binding pocket lying between the polymerase catalytictriad and NNRTI pocket [23] may be involved in AQsbinding to RT and in their ability ... the planar rings system guarantees a signiﬁcant in uence of the p–p stacking interaction with Trp229 on the orien-tation of the ligand in the binding site (Fig. 5A). Inter-actions between the ... the individual subdomains, whereas the p51 sub-unit is shown using the same color. The surface represents the position of the new binding pocket for RNase H inhibitors. Close-upof the binding...

Báo cáo khoa học: Creation of a new eye lens crystallin (Gambeta) through structure-guided mutagenic grafting of the surface of bB2 crystallin onto the hydrophobic core of cB crystallin pot

... spectra induced in the otherwiseachiral (and therefore non-dichroic) dye Stains-all in the presence of Gambeta, bB2 and cB crystallin. The dye Stains-all binding simulates calcium-binding in aABFig. ... inter-domainlinkers joining the N- and C-terminal domains in the two proteins are very different from each other in structure, as well as sequence, with the linker in cBbeing bent into a V-shape ... protein and Stains-all, the strength of the approximately 650 nm negative band(known as the J band) induced in Stains-all by itsbinding to Gambeta is intermediate to that of bB2 and cB crystallins....

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