Báo cáo khoa học: Combined use of selective inhibitors and ﬂuorogenic substrates to study the speciﬁcity of somatic wild-type angiotensin-converting enzyme docx

... Journal compilation ª 2006 FEBS 1781 Combined use of selective inhibitors and fluorogenic substrates to study the specificity of somatic wild-type angiotensin-converting enzyme Nicolas D. Jullien1, ... 1 and 2) validates the use of selective inhibitors as convenient tools to study the spe-cificity of the somatic form of ACE. Thus, with thisapproach, the catalytic property of each domaintowards ... potency and selectivity of the RXP inhibitors did not appear to vary to any great extenttoward these ACE enzymes. Such studies are relevantin the light of the differences exhibited by rat and mouse...

Báo cáo khoa học: Isoquinoline-1,3,4-trione and its derivatives attenuate b-amyloid-induced apoptosis of neuronal cells pdf

... [14]. The neurotoxicity of Ab seems to depend on its ability to aggregate, and the active portion of the Ab moleculeappears to be the amino acids 25–35 fragment [15,16].Most caspase-3 inhibitors ... ﬂow cytometry revealed the presence of a typ-ical sub-G1 peak indicative of an apoptosis ratio of 4.73%. As the duration of treatment increased to 48 and 72 h, the ratio increased to 19.91% and ... bymeasuring the recovery of enzymatic activity after arapid and large dilution of the enzyme inhibitor com-plex. If the inhibition is reversible, enzymatic activitywill recover to 90% of the initial...

Báo cáo khoa học: "Combining a Statistical Language Model with Logistic Regression to Predict the Lexical and Syntactic Difﬁculty of Texts for FFL" potx

... is the probability of meeting the token tiin French; and n is the number of tokens in a text.2. Deciding what is the best linguistic unit to consider. The equations introduced above use 21tokens, ... attention to the factthat the MLR model multiplies the number of pa-rameters by J − 1 compared to the PO model.Because of this, they recommend using the POmodel.6 Implementation of the modelsHaving ... wecan reduce this error, the lemma model mayprove to be the best predictor of the three.As a consequence of these considerations, wedecided to compute the difﬁculty of the text by us-ing Equation...

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... identify individualswith mild disease, thereby providing the missing data. The phenotype of XLA and the potential of BTK and ITK inhibitors The outcome of defective BTK signaling in humanshas ... for BTK [32] and ITK[33,34]. ITK inhibitors may potentially be used for the treatment of inﬂammatory diseases [34] and, as discussedbelow, may also become part of the anti-HIV therapeu-tic ... posi-tioned in the connecting loops. Approximately one-third of all mutations in the BTK gene are missense and some of these reduce the stability of the protein.This is exempliﬁed by mutations in the...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... measures the total dye amount associatedwith the cells, including the dye adsorbed to the sur-face of the cells. To avoid complication of the uptake studies byactive pumping of the dyes into the ... appear to be due to Pgp activity. Because the over-expression of MRP 1–5 did not signiﬁcantly reduce the amount of TMRM taken up into the cells, the obstacle to uptake of rhodamines into the cells ... Contrary to our expectations, under theseconditions, the free concentration of the dyes in the cytoplasm is low compared to the extracellular concen-trations of the dyes. Equilibration of the dyes...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... protein to preventdegradation. A common drawback of such approachesis the cost of the affinity resins used to isolate the fusion protein and the proteases required to liberate the protein of interest ... the peptide is bound in a concentrated mannerat the top of the column, or, if bound to the resinprior to packing the column, the salt gradient concen-trates the peptide on its way out of the ... chromatography and subsequent throm-bin cleavage [41]. The use of thrombin significantlyincreases the cost of the purification, and the require-ment for HPLC increases the length and complexity of the...

Tài liệu Báo cáo khoa học: Parvalbumin deficiency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of fiber specific proteins doc

... in SOL. These findings furthersupport the presence of distinct pathways regulatingfiber specific isoforms and metabolic enzymes, on the one hand, and mitochondrial biogenesis, on the other.Renewed ... of COX I, COX Vb and F1-ATPase b, betweenSOL and TA on the one hand and between PV– ⁄ – and WT TA on the other, revealed striking similarities.There were almost no differences in the mRNA and protein ... western blot analysis of the mitochondrial pro-teins cytochrome c oxidase subunits I and Vb (COX I and COX Vb,respectively), cytochrome c (Cyt c) and the beta subunit of the F1-ATPase isolated...

Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc

... 4-linked to the GlcNAc of a terminal Leadeterminant.As the C3aion is at m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminalGlcNAc. The position of the ... downﬁeld from the Fuc IX H5 proton. ForTFiLNO, the same temperature would have resulted in totaloverlap of the H2O signal with the Fuc X and XI H5protons. Therefore the temperature of 27 °C was ... out to verify the MSassignment of the iLNO core structures and its variouslyfucosylated analogues, and to determine their anomericconﬁgurations and linkages. The proton chemical shifts of 1178...

Tài liệu Báo cáo khoa học: Arginine ethylester prevents thermal inactivation and aggregation of lysozyme pptx

... analysed the thermal unfolding proﬁle of lysozyme in the presence of additives. Figure 3A shows the thermal unfolding curve of lysozyme in the presence of ArgEE as m onitored by DSC. In the absence of ... to Argis related to the ethylation of the main chain of the carboxylgroup. Figure 5B shows the amount of aggregation of lysozyme at pH 6.5 and 10.0 in the presence of Arg and ArgEE after heat ... but the near-UV CD data generated were too noisy to allowevaluation of the Tm.Charged states of Arg and ArgEEIn order to understand the importance of amphiphilicity,titration curves of Arg...

Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

... Attempts to clarify the stoichiometry by adding a small amount of the purifieddioxygenase to the reaction mixture with a large excess of the purified enzyme reported here to avoid the formation of 2,5-pyridinedicarboxylic ... time. The peak at 268 nm was assigned to 2,5-pyridinedicarboxylic acid based on the wavelength [10].Purification and properties of the purified enzyme The activity of the enzyme present in the crude ... absorption spectra of reaction products and the purified enzyme were recorded with a Beckman DU 650spectrophotometer. Fluorescence spectra of the purified enzyme and a cofactor released from the e nzyme...

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