Báo cáo khoa học: Critical roles of Leu99 and Leu115 at the heme distal side in auto-oxidation and the redox potential of a hemeregulated phosphodiesterase from Escherichia coli pptx
... DosH as a template
and using the following respective 5¢-sense primers: 5¢-gatga
gtcgggagACCcagctggagaaaaaag-3¢,5¢-gatgagtcgggagTTTcag
ctggagaaaaaag-3¢,5¢-ggacccgttttgcgACCtcgaaagtgagc-3¢,
and ... Journal compilation ª 2006 FEBS 1223
Critical roles of Leu99 and Leu115 at the heme distal side
in auto-oxidation and the redox potential of a heme-
r...
... oligonucleotides: Asp40Ala:
5¢-TGTTAATTAACGAAAATGC TGAAGTGATGTTTT
TC-3¢ (forward); 3¢-GAAAAACATCACTTC AGCATTT
CGTTAATTAAC-5¢ (reverse); Asp40Asn: 5¢-GGTGTT
AATTAACGAAAATAAC GAAGTGATGTTTTTCA
AC-3¢ (forward); ... Critical roles of Asp40 at the haem proximal side of haem-regulated
phosphodiesterase from
Escherichia coli
in redox potential,
auto-oxidation and cataly...
... deprotonating the C2 hydroxy group to
form an oxaloacetate intermediate and in facilitating
the hydride transfer from C2 to NADP
+
. After
decarboxylation of oxaloacetate, a general acid partici-
pates ... with the carbonyl oxygen of oxaloacetate and
facilitate the decarboxylation reaction to form enol-
pyruvate [21]. Our kinetic data on the D23 5A mutant
demonstrated...
... study, the
catalytic domains are indicated by capital letters ( A and
B) and the C-terminal peptides by lower case letters (a
and b), so that the wild-type enzymes are abbreviated as
Aa and Bb. The ... protein or alkaline phos-
phatase allowed the formation of tetramers associated
with an N-terminal fragment of ColQ [17]. However,
the catalytic domains are also invo...
... be
involved. In multicellular organisms, GATA-binding
factors play critical roles in development, including
cell-fate specification, regulation of differentiation, and
control of cell proliferation and ... loops of c-T1 and c-T2 pro-
mote the lateral interactions necessary to form the
c-TuRC ring template at cold temperatures [12,35]
and that the residue substitut...
... (5¢-atagacacgcaaacacaaatacaca
cactaaattaataatgaccggatcATGTACTTCCCCTTTTTAGG
CAGAT-3¢) and lip2 (5¢-cagtagagacatgggagatcccccgcgg
aattcgagctcggtacccgggTCATTCTTTATTTAGAGCATC
CAGC-3¢).
The sequences in lower-case ... was
generated by disrupting the ATF2 gene of CA10 with the
KanMX4 cassette, which confers G418 resistance. Primers
5¢ATF2-Kan 5¢-AGACTTTCAAACGAATAATAACTT
CAGCAATAAAAATTGTC...
... yields information on the
chemical nature of the individual radicals of the radi-
cal pair state, and their interaction with each other
and with their immediate surroundings.
EPR ⁄ ENDOR investigations ... Thus, replacement of His354 by alanine
leads to significant modification of the cofactor-
binding site at the 8a- methyl group and at the linkage
of the ribi...
... with
other flavin amine oxidases, including the overall fold of the amine oxidase
domain region and details in the active site that are relevant for amine
substrate oxidation.
Abbreviations
AOD, amine ... whereas in MAO A,
MAO B and MAO N the aromatic substrates bind in
a hydrophobic cavity that, in MAO B (Fig. 3A) , has a
bipartite nature and is shaped by a loo...
... state governs the association of
Yap4p and Lot6p. Although it appears likely that the
redox state of the FAD cofactor of NQO1 and
NQO2 plays a similar role in the mammalian system,
unequivocal ... protein [24], removing ubiquitin chains
for recycling [25,26] and opening an axial gate into the
20S catalytic chamber [27]. Whereas 26S proteasomal
degradation requires...