... 5¢-GACT
ACAAAGACCATGACGGTGATTATAAAGATCATGAC
ATCGATTACAAGGATGACGATGACAAGCTCATG-3¢
(sense), and 5¢- GTACAGCTTGTCATCGTCATCCTTG
TAATCGATGTCATGATCTTTATAATCACCGTCATGG
TCTTTGTAGTC-3¢ (antisense), and ligated into ... localization of MPP3, a novel
member of the MPP5 protein scaffold at the outer limiting membrane
(OLM), and of the DLG1 protein scaffold at the outer plex...
... entities. In other
words, the sub-tree is enclosed by the shortest
path linking the two entities in the parse tree (this
path is also commonly-used as the path tree fea-
ture in the feature-based ... trees. Their tree kernels require the match-
able nodes to be at the same layer counting from
the root and to have an identical path of ascend-
ing nodes from the...
... subtilis enzyme,
although this part of a-helix E is distorted and the
histidine points to the exterior of the proteins,
towards the solvent.
All these previous observations suggest that the
active ... conservation of the residues
known until now to be involved in the catalytic
mechanism, another significant difference is present
in the H. pylori enzyme. In the latter...
... located at the 5¢ untranslated region of the PABP-
mRNA. In this report, we have further characterized the interaction
between PABP and IMP1 with the ARS at the molecular level. The dissoci-
ation ... ribonucleoprotein complex at the ARS of PABP mRNA.
Abbreviations
ARC, autoregulatory ribonucleoprotein complex; ARS, autoregulatory sequence; IMP1, insulin-like growth factor...
... consistent
with the universal nature of the tRNA A- to P-site passage.
This requirement is somewhat released when the participa-
tion of the phosphate, rather than the base is required, as is
the ... forms a
scaffold that guides the motion from the A- to the P-site
(Fig. 3). This guidance, together with the front-side anchor-
ing, provides the precise path fo...
... are
myristoylated at the N-terminus, but they do not undergo a
Ca
2+
–myristoyl switch as do other NCS proteins such as
recoverin and hippocalcin [10,11]. However, they change
their conformation ... excess
Ca
2+
in the protein sample over that present in the
ultrafiltrate. The data were analysed as follows:
½Ca
2þ
free
¼ðR
f
=R
p
Þ½Ca
2þ
total
where R
f
is the radioactivit...
... requirement
to ensure that the effective dissociation constants
match the protein concentrations in vivo.
One can speculate on the hypothetical advantages
of a regulation mechanism based on the equilibrium
between ... sug-
gests that the conserved feature is, indeed, the forma-
tion of a dimer, and rules out the alternative
explanation that dimer formation is a necessary...
... by the fact that they are able to target
passenger proteins to the appropriate organelle. The
receptor machinery on the outer membranes of chloroplasts
and mitochondria is able to discriminate ... in
which isolated mitochondria and chloroplasts from pea are
mixed together and incubated with the precursor proteins,
and then the organelles are re-separated. The authors r...
... synthesis [15,16]. It follows that, unless there
are other unrecognized d
1
biogenesis proteins,
then NirF must catalyse the last step(s) in d
1
heme
synthesis.
The nature of these synthesis ... the resulting d
1
heme then being translocated to the periplasm. In the
case of P. pantotrophus it would be the substrate for
NirF that is translocated. In either case the transport...