Báo cáo khoa học: Conformational stability of 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus pptx

Báo cáo khoa học: Solution structure of Cu6 metallothionein from the fungus Neurospora crassa pptx

... hallmark of the MT structures is the dependence on the sequential position of the cysteine in the primary sequence, the identity of the coordinated metal andits c oordination number [14,48]. The ... isible in the T OCSY with the exception of the side c hain of Cys5(assigned from the N OESY), and a ll resonances of Gly1(not identiﬁed i n either experiment). Assignment of Gly1was not possible; ... positioning of the seven cysteineresidues with o ther members of t he MT family of proteins,none of the oth er structurally characterized MTs show the same fold. This is reﬂected in the r.m.s.d....

Báo cáo khoa học: Conformational stability of 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus pptx

... 8.5, we studied thermal stability of 17b-HSDcl from CD and UV meltingcurves, from which the temperature of denaturation(Td) and the van’t Hoff enthalpy of denaturation(DH°vH )of1 7b-HSDcl were ... guanidine hydrochloride;17b-HSDcl, 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus; DH°vH, van’t Hoff enthalpy of denaturation; SDR,short-chain dehydrogenase ⁄ reductase; Td, ... Rizˇner Conformational stability of 17b-HSDclFEBS Journal 273 (2006) 3927–3937 ª 2006 The Authors Journal compilation ª 2006 FEBS 3937 Conformational stability of 17b-hydroxysteroid dehydrogenase from...

Báo cáo khoa học: Conformational stability of neuroglobin helix F – possible effects on the folding pathway within the globin family potx

... byproteases. The limited proteolysis pattern also led to the establishment that no other potential cleavage sites of apoMb undergo hydrolysis, as a consequence of the stability of the helical fold of the ... etc.are the helix segments [12]. The folding scheme conﬁrms the absence of a stably folded helix F in native apoMb,in contrast with the N state of the holoprotein [12]. The stability of the globin ... on the apoand holo forms of the two proteins reveal the persistence of helix F in neu-roglobin even in the absence of heme. Conversely myoglobin shows ahigher mobility of the N-terminus of...

Báo cáo khoa học: Directed evolution of formate dehydrogenase from Candida boidinii for improved stability during entrapment in polyacrylamide ppt

... ⁄ v) PAA, the activity of the immobilisates was only $10% of the activity that had been expected from the amount of enzyme introduced into the matrix. This was in accord-ance with the many reports ... velocity of the reac-tion, vmax the maximum reaction rate, [x] the concentration of the two substrates, KMx the Michaelis–Menten constant of substrate x, and KINADH2 the inhibition constant of ... effect on stability. This indicatesa cooperation of the compounds in the deactivation of enzymes independent of the actual polymerization pro-cess. With regard to the identiﬁcation of FDH variantswith...

Báo cáo khoa học: Conformational heterogeneity of transmembrane residues after the Schiff base reprotonation of bacteriorhodopsin 15 docx

... time of 1.4 ms caused a signiﬁcant reduction in the intensity of resonance bR1 (Fig. 3). Other resonances were unaffec-ted within the limits of the signal-to-noise ratio of the spectrum. The ... 3Dstructure of the N-state [7] T170 faces the cytoplasmicchannel at the level of D96. Therefore, a cysteine sub-stitution would alter the hydrophobic pocket and the pKa of D96 and so shift the M–N ... itselfreprotonated from the cytoplasmic surface and a pro-ton is transferred from Asp85 to the proton releasesite. Analysis of the photomechanism has been revolu-tionized by the production of a family of...

Tài liệu Báo cáo khoa học: "Automatic Collection of Related Terms from the Web" pptx

... term.3. There is a deﬁnition or explanation of the term.4. There are several technical terms that are re-lated to the term.We have implemented the checking program of the ﬁrst two conditions in the ... number of pages that sat-isfy a given query. In case the query is a term, its hitis the number of pages that contain the term on the Web. We use the following notation.H(x)= the number of pages ... corpus isto select the appropriate passages from a documentset. We use the Web for the document set and se-lect the passages that describe s for the corpus. The actual procedure of compiling corpus...

Báo cáo khoa học: Increased susceptibility of b-glucosidase from the hyperthermophile Pyrococcus furiosus to thermal inactivation at higher pressures pptx

... the ﬁnding that at pH 10 and 75 °C, the inactivation of b-glucosidase also does not involveany loss of secondary structure [1].Conclusions The stability of the b-glucosidase from the hyperther-mophile ... b-sheetaggregate [21]. The thermal stability of b-glucosidase was assessedby plotting the temperature dependence of the peakintensity at 1618 cm)1(Fig. 4B). The melting point of the enzyme was estimated ... [9].In this work, we continued our study of the stability of the hyperthermophilic b-glycosidase from P. furio-sus. To assess the stability, we monitored the changesin secondary structure with...

Báo cáo khoa học: Conformational stability and multistate unfolding of poly(A)-speciﬁc ribonuclease docx

... located on the top of the substrate-binding site in the nuclease domain of the other subunit, whichimplies that it may participate in the binding with the poly(A) substrate [14]. In the primary ... increaseoccurred in the size of the protein. Meanwhile, the peak area of the eluted proteins in the SEC proﬁle wasgreatly reduced compared with that of the native pro-tein, which might be caused by the appearance ... with the addition of variousamounts of NaCl. A protein concentration of 0.4 mgÆmL)1was used to highlight the off-pathwayprocess. Similar to the results of the 0.1 mgÆmL)1sam-ple, the ﬂuorescence...

Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological signiﬁcance docx

... [54].Functional stability of Ngb The conformational stability of the apoNgb structurein acid is in agreement with the enhanced functionalacid stability of the holoprotein. In fact, Ngb retains the ability ... we have shown that the acid stability of the Ngb fold must be ascribed to the intrinsic stability of the apoprotein polypeptidechain and is not dictated by the presence of the intra-molecular ... Mb. The denatur-ation of the proteins was monitored by recording the decrease in the ellipticity at 222 nm in the far-UV CD spectra of the protein in the presence of increasing concentrations of...

Báo cáo khóa học: Conformational changes of b-lactoglobulin in sodium bis(2-ethylhexyl) sulfosuccinate reverse micelles A ﬂuorescence and CD study docx

... formation, which leads to the movement of the charged groups of the protein in order to keep themselvesneutral.As a means of testing the role of the surfactant on the protein conformations, ... content. The radius of the volume element [Ri¼ (3Vi/4pNa)1/3] gives a measure of the proximity of the quenchermolecule to the ﬂuorophore. The calculated values arealmost within the van ... of the decrease of the dielectric constant of the surrounding medium [45] because, in several alcoholicmixtures, the cluster (micelle-like assembly) formation of each solvent in the excess of...

Xem thêm