Báo cáo khoa học: Enhancing thermostability of maltogenic am

Báo cáo khoa học: Enhancing thermostability of maltogenic amylase from Bacillus thermoalkalophilus ET2 by DNA shufﬂing pdf

... The Authors Journal compilation ª 2006 FEBS 3345 Enhancing thermostability of maltogenic amylase from Bacillus thermoalkalophilus ET2 by DNA shufﬂing Shuang-Yan Tang 1 , Quang-Tri Le 1 , Jae-Hoon ... Thermostable and alkalophilic maltogenic amylases of Bacillus thermoalkalophilus ET2. Biocatal Biotransform 23, 79–87. Enhancing MAase thermostability by...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding pot

... a 3-A ˚ dissociation of subunits interacting by a1. The base of dTTP is inserted between the a1-helix of two subunits and is stacked between the rings of Phe18 and Phe34 from the adjacent subunit ... enzyme. The pattern of interaction of ATP with TKs from Gram-negative bacteria remains to be evaluated. O4 of the thymine makes a hydrogen bond with the main- chain nitrogen...

Ngày tải lên : 23/03/2014, 09:21

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Tài liệu Báo cáo khoa học: Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation pdf

... of the WT enzyme and between those of G194P and G198P. Thermostability of WT and mutant enzymes The thermostability of the WT and mutant enzymes was determined by measuring residual activity after incubation ... stability of Ochr-MPH; however, replacing G198 of Ochr-MPH with proline did not improve the thermostability. The thermostability of the double point mutant (G1...

Ngày tải lên : 15/02/2014, 01:20

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Báo cáo khoa học: "Enhancing Performance of Lexicalised Grammars" pdf

... the efﬁciency of a parser with a possible trade-off of los- ing correct parses. For example, the noun phrase reading of The dog barks is a correct parse, although unlikely. By blocking the use of barks ... jh5 section of the treebank released with the ERG. This text consists of edited written English in the domain of Norwegian hiking instructions from the LOGON project (Oepe...

Ngày tải lên : 17/03/2014, 02:20

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Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... fraction of bound 32 P-labeled DNA (R) is the ratio of the fraction of bound 32 P-labeled DNA in the presence of the competitor DNA (cpm bound ⁄ cpm total ) to the fraction of bound 32 P-labeled DNA ... efﬁciency of the reference unmodiﬁed DNA and the competitor damaged DNA to the MTase. The K d values were calculated by dividing K r d (see below) by the experi- me...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx

... half-life of % 13 and 33 min, respectively. Substrate speciﬁcity analysis An analysis of the substrate speciﬁcity for DNA of the enzymes shows that they both cleave plasmid DNA, dsDNA and ssDNA as ... calculated as described by Lonhienne et al. [37]. Activation energy, E a , was extracted from the slope of the linear regression curve obtained from an Arrhenius plot of 1 ⁄...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... exhibit a wide range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structural diversity of skeletons in their ... respectively, by GC-MS. Co-expression of CYP93E1 and b-amyrin synthase in S. cerevisiae yielded olean-12-ene-3b,24-diol. This is the ﬁrst identiﬁcation of triterpene hydroxylase c...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf

... lm. A B Fig. 5. Expression of iNOS in duodenum tissue of mice. (A) Quanti- fication of iNOS mRNA induction by flagellin in whole tissue and microdissected epithelium from villi assessed by real-time PCR. ... respectively. Specificity of PCR was checked by ana- lyzing the melting curve. Relative mRNA levels were determined by comparing (a) the PCR cycle threshold between cDNA...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... alignment of the C-terminal extension of PhKc. Sequences were obtained for both vertebrate and nonvertebrates by using sequence from 296 to 386 of PhKc from rabbit muscle. Identities are shown by the ... 1.2 molar ratio of Ca 2+ ⁄ CaM to PhK5 had been reached. The inset shows an expanded view of the indi- cated area of the spectrum. (Bottom) An overlay of spectra from...

Ngày tải lên : 19/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

... half-maximal amounts of pPacUSp1 (50 ng, left panel) or increasing amount of Ets-1 with 50 ng of either Sp1 or Sp3. (B) Assay of fgl2 promoter activity upon cotransfection of thresholds amount of both Sp1 ... site of fgl2 mRNA. (A) 5¢-RACE of fgl2 mRNA from macrophages. Stars indicate the 5¢-ter- minus of 33 clones isolated after 5¢-RACE. (B) Primer extension analysis of...

Ngày tải lên : 20/02/2014, 11:20

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