Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... November
2006)
doi:10.1111/j.1742-4658.2006.05596.x
The zinc-finger cellular nucleic acid binding protein (CNBP) is a strikingly
conserved single-stranded nucleic acid binding protein essential for normal
forebrain formation during mouse and ... 2006 The Authors Journal compilation ª 2006 FEBS 497
In vitro embryonic developmental phosphorylation of the...
... [22–25]. Since a functioning zinc-bind-
ing domain may be present in the Ab sequence of C99,
we hypothesized that zinc may affect the oligomeriza-
tion state of C101-3FLAG. Therefore, the molecular
mass ... APP
intracellular domain (AICD or e-CTF) by cleaving 9–7
amino acids from c 40 and 42 sites at the e site [1–3].
Several lines of evidence support the pathogenic role...
... T7, etc.) on the DNA exten-
sion reaction by using
32
P-labeling primer or template
in the presence or absence of DNA -binding agents
(DDIs and doxorubicin). The DNA bulge binding
of both compounds ... For example, the change in T
m
(DT
m
) for the
ATT bulge increased by 3.4 and 1.7 °C in the presence
of DDI-1A and DDI-1B, respectively. The increase...
... statement and
suggests that the RNA binding site is still available and its
binding affinity is not altered by the presence of DNA in the
DNA binding site.
Interestingly, when 3 n
M
RNA and 10 n
M
DNA ... explain the
complete inability of a saturating amount of DNA substrate
to inhibit RNA splicing (Fig. 6B) and therefore it seems
reasonable to make the work...
... the protein into the targeting and translocating
pathway by interacting with the membrane and other
components of the cellular secretory machinery [1]. The
signal peptides of secreted proteins ... demonstrated for the first time that
the self-cleavage of SPase I is happening within the
S. pneumoniae cells in all the growth phases, and the
cells maintai...
... g for 10 min. In vitro selection for
mAb M2 binding was by adding 200-lL aliquots of the
supernatant to the selection matrix and incubating at room
temperature for 30 min. Unselected protein plasmid ... split-
protein sensor for the detection of protein protein
interactions [25].
In E. coli PRAI, the loop connecting b-strand 6 with
helix 6 (‘loop 6’) forms...
... coli and studied its substrate preferences, the factors
in uencing its activity and the nature of suitable metal
cofactors and inhibitors in vitro.
Materials and methods
Chemicals
The following ... [aliphatic-(X)3-H-(X)2/3-L/T/S] proposed by
Fisher and Rich [20] for quinone binding sites. Although
speculative at this stage, this consensus sequence was found
i...
... guanine nucleotide releasing protein ND – Rat IGF binding protein 5 ND –
Rat FGF receptor activating protein 1 ND –
Rat PKC delta -binding protein ND –
Rat cellular retinoyl -binding protein ND ... proteins
Rat RY2G5 potential ligand binding protein ND BG Rat RYF3 (vomeromodulin) ND LNG
Rat mucin-like protein 1,2,3 S Rat RYA3 potential ligand binding protein ND BG
Ra...
... sequencing,
and protein biochemical characterization of the initial
acetylene hydratase is inevitable. In case of the other, more
common enzymes, which are involved a genomic sequence
analysis and ... from
acetaldehyde are incorporated into the alcohol formation in
the other metabolite. The assumption was proved by adding
acetaldehyde (0.5 m
M
and 5 m
M
) to the...
... signal
transduction by interaction with cytoplasmic proteins such
as TAB1 [61] or mitogen-activated protein kinase kinase
kinase [54]. These distinct antagonizing mechanisms of
Smad7 open up the question whether ... Smad2 and Smad3 share 92% amino
acid identity, they are functionally distinct. A short amino
acid sequence in the MAD homology 1 (MH1) domain of
Smad2 is respo...