... Hough1 and Michael J. Danson11 Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, UK2 Department of Biology and Biochemistry, University of Bath, ... and pyruvate [14]. In the current paper, we report the clon-ing and expression of the E2 and E3 genes of the sameoperon from T. acidophilum, and the in vitro assembly and characterization of ... However, detection of E3 and lipoic acidin various archaea [10–12] led to the discovery of aputative OADHC operon in Haloferax volcanii [13] and our subsequent detection of similar putative...