Báo cáo khoa học: Substrate and inhibitor speciﬁcity of Mycobacterium avium dihydrofolate reductase pptx

... compilation ª 2007 FEBS Substrate and inhibitor speciﬁcity of Mycobacterium avium dihydrofolate reductase Ronnie A. Bo¨ck1,*, Jose L. Soulages2 and William W. Barrow11 Department of Veterinary Pathobiology, ... (1983) Directedmutagenesis of dihydrofolate reductase. Science 222,782–788.2 Hartman PG (1993) Molecular aspects and mechanism of action of dihydrofolate reductase inhibitors. J Chemo-ther ... of aspartate 27 of dihydrofolate reductase from Escherichia coli in interconversion of active and inactive enzyme conformers and binding of NADPH.J Biol Chem 265, 5579–5584.19 Birdsall B, Andrews...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... localization of the differentepimers. Taken together, these results allow a discus-sion of the existence of enzyme(s) converting l-tod- residues in the peptide chain in terms of substrate speciﬁcity and ... the elution zone of CHHs and VIHs from lobster sinus glands was per-formed: 10 lL aliquots of each fraction were pipetted intriplicate into the wells of a microtitre plate and driedunder vacuum. ... Ollivaux C & Soyez D (2000) Dynamics of biosynthe-sis and release of crustacean hyperglycemic hormoneisoforms in the X-organ-sinus gland complex of thecrayﬁsh Orconectes limosus. Eur J...

Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... mRNA and protein levels of key pro-fibrotic proteins like PDGFR-a and -b, TGFb1, and TIMP-1 and -2 in the liver of transgenic mice. PDGF-Ctreatment increased both mRNA and protein levels of the ... model of thePDGF-C growth factor domain indicates the disulfidebridges in PDGF-C to consist of Cys250 and 294,Cys280 and 335, and Cys287 and 337, and the inter-monomeric bonds to consist of Cys274 ... tuning of PDGF and PDGFR isoformexpression and regulationExpression of both receptors and each of the fourPDGFs is under independent control, giving thePDGF ⁄ PDGFR system a high degree of combinatorialflexibility....

Tài liệu Báo cáo khoa học: Changes in purine speciﬁcity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx

... structures of the tandem GAFdomains of PDE2 and cyaB2 do not reveal how cNMP speciﬁcity is enco-ded, we generated chimeras between the tandem GAF domains of cyaB1 and PDE2. Both bind the ligand in ... [2,3]. The lig- and for the tandem GAF domains of PDE2, PDE5,PDE6, and PDE11 is cGMP and that for PDE10 iscAMP. Usually ligand-binding enhances catalyticactivity [4–6]. Two ACs of the cyanobacterium ... cyaB1 and rPDE2 tandem GAF domains that dis-criminate between the adenine and guanine of cAMP and cGMP as primary ligands.Previous experiments investigating cGMP binding toGAF domains of mammalian...

Báo cáo khoa học: Molecular basis for speciﬁcities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases pptx

... (1977) Mechanism of action of adenosylcobalamin:glycerol and other substrate analogues as substrates and inactivators for propanediol dehydratase – kinetics, ste-reospeciﬁcity, and mechanism. ... (DR), and GDR (GR) are indicated with arrowheads to the right of the gels, and theircomplexes 1–4 to the left. Bands 1 and 2 correspond to 1 : 2 and 1 : 1 DD–DDR complexes, respectively, and bands ... lanes 3 and 4). In the presence of ATP, the new major band with DDR did notappear, whereas a part of the new band with GDRremained (Fig. 4C, lanes 3 and 4). To identify the newmajor band formed...

Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx

... synthesized is 35% of the total amount of plasma membrane proteins, in comparison with thelevel of H+-ATPase which represents 20–50% of thetotal amount of plasma membrane proteins [23,24], and assuming ... 5537mechanisms of adaptation (storage mobilization,expression of exporter, adaptation of membrane per-meability), their timing and the modeling of biomassproduction using an energy balance and the ... fractions of O2 and CO2inthe gas; /L(m3Æs)1) and /G(molÆs)1) are the volumetricmedium and gas ﬂow rates; klaO2 and klaCO2[s)1] are thegas-liquid transfer coefﬁcients for O2and...

Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt

... D esign and synthesis of a p otent c yclic ana-logue of the myelin basic p rotein epitope MBP72-85: importance of the Ala81 carboxyl group and of a cyclic conformation forinduction of experimental ... side chain of Arg78 (Hg) and Glu82 (Hc1 )and the medium-range NOE between the side chains of Asp81(Hb) and Arg78 (Hg and Hc)]. In addition, Glu82 i nteractswiththesidechainofLys75.The ... chain of Arg78. The side chain of Arg78 is lesswell deﬁned, as in the case of aqueous solution, because of the absence of interactions with the s ide chains of A sp81 (inthe agonist analogue) and...

Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp. strain HE5 ppt

... Isolation and purification of the pro-tein and cloning and sequencing of its operon. J BiolChem 267, 14193–14203.27 Uhlmann H, Kraft R & Bernhardt R (1994) C-Terminalregion of adrenodoxin ... the microsomal type of P450s like P450BM3[24] and are reduced by a diflavin reductase. This is the firstreport on the heterologous expression and purification of all components of a P450 system ... indicate less efficientincorporation of the Fe3S4cluster and ⁄ or lowerstability of the cofactor, compared with CHis-Fdmor and wt-Fdmor.EPR-spectroscopy of oxidized wt-Fdmorrevealed asingle...

Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

... factor of 18.8% and an Rfree of 22.3%with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2). The resulting model isdepicted in Fig. 2. The Ca backbones of the ... b-strands are labeled.Fig. 3. B-factor plots of the main chains of the two molecules of CDase in the asymmetric unit. The averages of molecules A (solid line) and B (brokenline) are 39 A˚2 and ... [38] and in the CD-degrading TVA-II [13], but not in the CD-degrading enzymes ThMA and BaCD nor in the majority of a-amylases. Ca-II is located inthe loop between a1anda2 of domain A (Fig. 2) and Table...

Báo cáo khoa học: Stability and ﬁbril formation properties of human and ﬁsh transthyretin, and of the Escherichia coli transthyretin-related protein potx

... 2011Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia colitransthyretin-related proteinErik Lundberg1, Anders Olofsson2, Gunilla T. Westermark3 and ... S & Shoham M (1999)Crystal structure of a thermophilic alcohol dehydroge-nase substrate complex suggests determinants of substrate specificity and thermostability. Proteins 37,619–627.48 ... properties of sbTTR and ecTRP in vitro, and compared the resultswith those of hTTR.Our results showed that hTTR, sbTTR and ecTRPcan form fibrillar structures, but under different solu-tion and temperature...

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