Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf

... the deformability required for the formation of the Early protein 2 C-terminal DNA- binding domain DNA complexes of var-ious types is based not only on the rigidity of the base sequences in the DNA ... Papillomavirus E2 DNA- binding domain simulationsFEBS Journal 274 (2007) 2385–2395 ª 2007 The Authors Journal compilation ª 2007 FEBS 2395 Molecular dynamics of the DNA- binding domain of the papillomavirus ... distribution of molecular ﬂexi-bility. The mechanical properties that characterize the two proteins, together with the different structural andconformational features of the spacer regions in the DNA target...

Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt

... equilibriumstate. The overall conformation of the helix is of B type.Average value of the major groove width is 16.8 A˚ for DDwtand 18.0 A˚ for DDmut, while the average widths of the minor groove are ... of the aforementioned sequence is because of the provenefﬁcacy of the designed PNA to down-regulate the gene expression of the mutated allelle, while sustaining the transcription of the wild-type ... parallelPNA DNA hybrid deviates from that of DNA duplex[11], comparison of the MD structure of parallelPNA DNA [36] with the B DNA indicates someamount of similarity especially with respect to lack of X-displacement....

Báo cáo khoa học: Molecular organization and force-generating mechanism of dynein pot

... shear motionsbetween the helices of the stalk coiled coil. The crystallographic analysis of the motor domain of Dictyostelium dynein provides the evidence for the structural information pathway ... disrupted coordination between the two motor domains. The results now shed light on the role of the tail for the gating mechanism.Crystal structures of the dynein motor domain intwo different nucleotide ... motifs of AAA1by vanadate-mediated photocleavage [80] of the HC of axonemal dynein. Further strong support for a func-tional role of the Walker motif of AAA1 is provided by molecular dissection of...

Báo cáo khoa học: C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactions ppt

... see Fig. 3 for the amino acid sequence of CTC). These fragments show that one of the twojuxtaposed Cys residues at positions 120 and 121forms a disulﬁde with Cys148 and the other forms adisulﬁde ... proteolysis of CTC. The amino acid sequence of human CTC is in upper-case letters, and the sequence of the S-tagis in lower-case letters. The numbering refers to the positions in the full-length ... Recently, the Brichos domain has been found also in other proteins,including a protein (TFIZ1) that binds trefoil domains[13]. The Brichos domain may be involved in foldingand processing of the...

Báo cáo khoa học: Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase a is required for Src activation doc

... structures of LAR andCD45, another role of the D2 domain was proposed:stabilization of the D1 domain [7,22]. These interactionssuggest that the D2 domains function as regulators of the activity of the ... CD45-D2 isalso critical for substrate recruitment of TCR-zetain vivo, because replacement of the membrane-distal domain of CD45 with the LAR D2 domain abo-lishes the binding of TCR-zeta [47].It ... show that the Arg554 residue is essential for the catalytic activity of RPTPa-D2.Catalytic activity of the RPTPa D2 domain isrequired for Src binding and activationIt is well known that the membrane...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... 4452–4463 ª 2010 The Authors Journal compilation ª 2010 FEBS 4453 the a-hydrogen of the substrate, and the other isG374, which forms the binding pocket for the branched-chain acyl moiety of substrate ... the skuphenotype. Because G376V was a novel mutation inIVD, the effect of the mutation was further investi-gated by molecular modelling. Together with the previ-ously reported traits of the ... combination of or and sku phenotypes. The lowerpart indicates the representative results of genomic DNA directsequencing of PCR products harbouring the SNP 1127G>T of BmIVD from each of the three...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... from the cell body to the axon terminal in the sinus gland, as a result of late and progressiveisomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonaltract ... microscopystudy of the neuronal endings in the sinus gland todetermine the subcellular localization of the differentepimers. Taken together, these results allow a discus-sion of the existence of enzyme(s) ... 4G).DiscussionAlthough the existence of two VIH isoforms with iden-tical sequence, molecular mass and isoelectric pointhas been known for more than 15 years [22], the nat-ure of the difference between the two...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... that play key roles in the accumulation of inﬂammatory cells at the site of inﬂammation. There-fore, chemokines and chemokine receptors are consid-ered to be therapeutic targets in several chronicKeywordschemokine ... CCL13enhances the proliferation of FLS [13,47]. Our groupreported that the proliferation of FLS by MCP-4 ⁄ CCL13 is dependent on activation of the extracellu-lar regulated kinase MAPK. The activation of ... protective against the development of RA [61]. The gene copy number variations of CCL3-like 1(CCL3L1), a nonallelic isoform of CCL3 encoded bydifferent genes and a potent ligand for CCR1 andCCR5,...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... account for the promiscuous nature of the synthesis functions performed by the prim–pol domain and to discriminate between the functions of in vitro primase and polymerase.Another finding of our ... asRepA [18]. Bioinformatic analyses of the predictedamino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded ... subdomains, we man-aged to model the entire domain without it.Another significant finding concerns the nature of the acid residues within the active site of Rep245. The carboxylate triad of Rep245...

Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx

... spectra of the M domain of human eRF1. The numbering of the residues corresponds to that of the fulleRF1 protein. (A) The Gly region of the 1H,15N-HSQC spectrum of the M domain of human eRF1 ... analysis The conformations of the backbone and side-chains of the M domain of human eRF1 are well deﬁned except for the residues (175–189) in the GGQ loop. The back-bone conformation of this ... beinvolved in transduction of the signal from the N-ter-minal domain, upon the recognition of the stop codon,to the M domain for subsequent initiation of the hydrolysis of peptidyl-tRNA ester bond....

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