Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: An unexpected role for quinone reductases as regulators of proteasomal degradation pptx

... preventing the generation of potentially harmful semiquinones. In recentyears, an additional role of quinone reductases as regulators of proteaso-mal degradation of transcription factors and possibly ... ﬂavoproteins in molecular cell biology: An unexpected role for quinone reductases as regulators of proteasomal degradation Sonja Sollner and Peter MacherouxTechnische Universita¨t Graz, Institut ... extendedregions of intrinsic disorder [64].From mammalian cells to yeast:a homologous system in a unicellularorganismAll of the initial studies indicating a role for QR in stabilizing transcription...

Báo cáo khoa học: New roles of ﬂavoproteins in molecular cell biology: Blue-light active ﬂavoproteins studied by electron paramagnetic resonance pptx

... was identi-fied as an intermediate in the formation of 64PPs[64,69]. This putative repair mechanism of (6-4) pho-tolyases requires one histidine acting as a protonacceptor and the other as ... Universita¨t Berlin, GermanyIntroductionUltraviolet light (k £ 300 nm) is known to induce theformation of covalent linkages between pairs of thy-mine and cytosine bases in cellular DNA. The ... His354 by alanineleads to significant modification of the cofactor-binding site at the 8a-methyl group and at the linkage of the ribityl side chain. Hence, as a first result, struc-tural information...

Báo cáo khoa học: New roles of ﬂavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin pot

... MINIREVIEW New roles of flavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatinFederico Forneris1, Elena Battaglioli2, Andrea Mattevi1and Claudia Binda11 ... flavin amine oxidases, including the overall fold of the amine oxidasedomain region and details in the active site that are relevant for aminesubstrate oxidation.AbbreviationsAOD, amine oxidase ... the involvement of LSD1 in an ever-growing number of transcriptionalprotein complexes emphasizes the central role of thisflavoenzyme in the chromatin environment. LSD1activity has also been linked...

Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf

... activation of caspase 3 in human non-small cell lung cancer cell line PC-9. To investigate the signaling routeby which AG1478 induced apoptosis, we examined the activation of c-JunN-terminal kinase ... FEBS 1255kinase (ERK) 1 ⁄ 2 plays an essential role in EGF-induced cell growth; and the phosphatidylinosi-tol 3-kinase (PtdIns3K) pathway is also important for cell growth and cell survival. ... apoptosis in a variety of cancer cells of different histotype [51]. Chang et al.recently reported that an increase in Fas proteinexpression might be the molecular mechanism bywhich geﬁtinib induces...

Báo cáo khoa học: The chloroplast ClpP complex in Chlamydomonas reinhardtii contains an unusual high molecular mass subunit with a large apical domain doc

... lineage of plants. ClpP genes are also found in Cyanobacteria[22] and in the genome of the Cyanophora cyanelle, an ancestral chloroplast. In the green alga Chlamydomonasreinhardtii and in vascular ... parvum, an Apicomplexan that has lostits apicoplast, also has lost both ClpP and ClpR [38,39]. In any event, apicomplexan ClpP appears as an interes-ting target for the design of antiparasitic ... resulting peptide masslists were searched against the latest version of the NCBInonredundant database using the search engine ProFoundand a database of Clp proteins from Chlamydomonas made in- house,...

Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt

... change of the Fe(II) spin state and noreplacement of any of the two axial ligands of the iron,His18 or RNO, by an amino acid side-chain of theantibody protein.Binding site topology of antibody ... containing sulfur werechosen as substrates, as they play an important role in medicine and agriculture. It has been reported that  10–15% of medicinal and veterinary products and  33% of synthetic ... models for those formed not only in vitro, but also in vivo, during the oxidative metabolism of drugs containing an amine function, such as amphetamineor macrolids [20], and which lead to an inhibition...

Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc

... temperature of the solution was maintainedat 25 °C, the reaction mixture was i ncubated for 10 minprior to spectroscopic measurements.Cyanide bindingThe association rate of cyanide to haem was observed ... Met95Table 2. Cyanide binding rates, r edox potentials and auto-oxidationrates of the Asp40Ala and Asp40Asn mutants of Ec DOS PAS. Cyanidebinding rate, experimental errors are within 20%; kox, autoxidationrate; ... (2001) PAS kinase: an evolutionarily conservedPAS domain-regulat ed serine/t hreonine k inase. Proc.NatlAcad.Sci. USA 98, 8991–8996.8. Jain, R. & Chan, M.K. (2003) Mec hanisms of ligan d...

Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx

... E119 can act as ageneral base for deprotonation of intermediates, as proposed by Berthold et al. [3,4] for OfODC. Thetyrosine residues 118 and 478 (the latter in an uncom-mon side-chain conformation) ... mainchain nitrogens of residues I322 and I303 interact withnitrogen atoms of the adenine ring and the c-carboxylgroup of the side chain of residue D302, the d and xnitrogen atoms of the guanodino ... 3,4-dimethylthiazoliumion in water and ethanol. J Am Chem Soc 92, 5707–5716.23 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of proteinutilizing the principle of protein–dye...

Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafﬁcking of human acetylcholinesterase and butyrylcholinesterase potx

... Cunningham JM, Massou-lie´J & Sanes JR (1999) Genetic analysis of collagen Q: roles in acetylcholinesterase and butyrylcholinesteraseassembly and in synaptic structure and function. J Cell Biol ... domains and C-terminaltail peptides in the oligomerization and secretorytrafﬁcking of human acetylcholinesterase andbutyrylcholinesteraseDong Liang1,2, Jean-Philippe Blouet1, Fernanda ... secretion was increased by deletion of thet peptides, and that it was more efﬁcient with a than with b. The patterns of oligomers were similar for Aa and Ab, as well as for Ba and Bb, indicat-ing...

Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc

... replacement has only beenobserved in E. focardii and in the psychrotolerantE. crassus [34, present study]. By contrast, the similarsubstitution of alanine for arginine 72 in the c-tubu-lins of T. ... focardiic-tubulins in total cell extracts and in subfractionsenriched in basal bodies or in micronuclei using anti-(human c-tubulin) and anti-c-T1 sera. Figure 7 showsthat the human antibody recognized ... of three E. focardii cells after stain-ing with antibodies directed against a- and c-tubulins (cell 1, Fig. 6A–H; cell 2, Fig. 6I–L; cell 3, Fig. 6M–P).The anti-(human c-tubulin) serum stained...

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